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Mouse Anti-MAPK8IP1 Recombinant Antibody (50/JIP-1) (CBMAB-0221-WJ)

This product is a mouse antibody that recognizes MAPK8IP1. The antibody 50/JIP-1 can be used for immunoassay techniques such as: WB.
See all MAPK8IP1 antibodies

Summary

Host Animal
Mouse
Specificity
Mouse, Rat
Clone
50/JIP-1
Antibody Isotype
IgG1
Application
WB

Basic Information

Immunogen
Mouse JIP-1 (amino acid 180-384)
Specificity
Mouse, Rat
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
MITOGEN-ACTIVATED PROTEIN KINASE 8 INTERACTING PROTEIN 1
Introduction
This gene encodes a regulator of the pancreatic beta-cell function. It is highly similar to JIP-1, a mouse protein known to be a regulator of c-Jun amino-terminal kinase (Mapk8). This protein has been shown to prevent MAPK8 mediated activation of transcription factors, and to decrease IL-1 beta and MAP kinase kinase 1 (MEKK1) induced apoptosis in pancreatic beta cells. This protein also functions as a DNA-binding transactivator of the glucose transporter GLUT2. RE1-silencing transcription factor (REST) is reported to repress the expression of this gene in insulin-secreting beta cells. This gene is found to be mutated in a type 2 diabetes family, and thus is thought to be a susceptibility gene for type 2 diabetes. Diseases associated with MAPK8IP1 include Diabetes Mellitus, Noninsulin-Dependent and Sarcomatoid Squamous Cell Skin Carcinoma. Among its related pathways are ERK Signaling and MAPK signaling pathway. An important paralog of this gene is MAPK8IP2.
Entrez Gene ID
Mouse19099
Rat116457
UniProt ID
MouseQ9WVI9
RatQ9R237
Alternative Names
IB1; JIP1; JIP-1; PRKM8IP
Function
The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Required for JNK activation in response to excitotoxic stress. Cytoplasmic MAPK8IP1 causes inhibition of JNK-regulated activity by retaining JNK in the cytoplasm and inhibiting JNK phosphorylation of c-Jun. May also participate in ApoER2-specific reelin signaling. Directly, or indirectly, regulates GLUT2 gene expression and beta-cell function. Appears to have a role in cell signaling in mature and developing nerve terminals. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins. Functions as an anti-apoptotic protein and whose level seems to influence the beta-cell death or survival response. Acts as a scaffold protein that coordinates with SH3RF1 in organizing different components of the JNK pathway, including RAC1 or RAC2, MAP3K11/MLK3 or MAP3K7/TAK1, MAP2K7/MKK7, MAPK8/JNK1 and/or MAPK9/JNK2 into a functional multiprotein complex to ensure the effective activation of the JNK signaling pathway. Regulates the activation of MAPK8/JNK1 and differentiation of CD8(+) T-cells.
Biological Process
JUN phosphorylationIEA:Ensembl
Negative regulation of intrinsic apoptotic signaling pathwayIEA:Ensembl
Negative regulation of JUN kinase activityISS:UniProtKB
Positive regulation of JNK cascadeISS:UniProtKB
Regulation of CD8-positive, alpha-beta T cell proliferationISS:UniProtKB
Regulation of JNK cascadeISS:UniProtKB
Regulation of transcription, DNA-templatedIEA:Ensembl
Vesicle-mediated transportISS:UniProtKB
Cellular Location
Cytoplasm
Cytoplasm, perinuclear region
Nucleus
Endoplasmic reticulum membrane
Mitochondrion membrane
Accumulates in cell surface projections. Under certain stress conditions, translocates to the perinuclear region of neurons. In insulin-secreting cells, detected in both the cytoplasm and nucleus (By similarity).
Involvement in disease
Diabetes mellitus, non-insulin-dependent (NIDDM):
A multifactorial disorder of glucose homeostasis caused by a lack of sensitivity to the body's own insulin. Affected individuals usually have an obese body habitus and manifestations of a metabolic syndrome characterized by diabetes, insulin resistance, hypertension and hypertriglyceridemia. The disease results in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
PTM
Phosphorylated by MAPK8, MAPK9 and MAPK10. Phosphorylation on Thr-103 is also necessary for the dissociation and activation of MAP3K12. Phosphorylated by isoform 1 and isoform 2 of VRK2. Hyperphosphorylated during mitosis following activation of stress-activated and MAP kinases.
Ubiquitinated. Two preliminary events are required to prime for ubiquitination; phosphorylation and an increased in intracellular calcium concentration. Then, the calcium influx initiates ubiquitination and degradation by the ubiquitin-proteasome pathway.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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