Rabbit Anti-MAPKAP1 Recombinant Antibody (CBXS-5434) (CBMAB-S2645-CQ)

This product is a rabbit antibody that recognizes MAPKAP1. The antibody CBXS-5434 can be used for immunoassay techniques such as: WB, IP.
See all MAPKAP1 antibodies

Datasheet

Summary

Host Animal

Rabbit

Specificity

Human, Rat, Monkey

Clone

CBXS-5434

Application

WB, IP

Basic Information

Specificity

Human, Rat, Monkey

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Mitogen-Activated Protein Kinase Associated Protein 1

Introduction

This gene encodes a protein that is highly similar to the yeast SIN1 protein, a stress-activated protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been described. Alternate polyadenylation sites as well as alternate 3' UTRs have been identified for transcripts of this gene.

Entrez Gene ID

Human	79109
Monkey	702599
Rat	296648

UniProt ID

Human	Q9BPZ7
Monkey	G7NEQ6
Rat	Q6AYF1

Alternative Names

Mitogen-Activated Protein Kinase Associated Protein 1; Mitogen-Activated Protein Kinase 2-Associated Protein 1; Stress-Activated Protein Kinase-Interacting 1; SAPK-Interacting Protein 1; TORC2 Subunit MAPKAP1; MSIN1; MIP1; SIN1; Stress-Activated Map Kinase Interacting Protein 1;

Function

Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAS signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. Involved in ciliogenesis, regulates cilia length through its interaction with CCDC28B independently of mTORC2 complex.

Biological Process

Cellular response to nutrient levels1 PublicationIC:ComplexPortal
Cytoskeleton organization1 PublicationIC:ComplexPortal
Negative regulation of apoptotic process1 PublicationIC:ComplexPortal
Negative regulation of Ras protein signal transductionManual Assertion Based On ExperimentIMP:UniProtKB
PhosphorylationManual Assertion Based On ExperimentIDA:ComplexPortal
Positive regulation of cell growth1 PublicationIC:ComplexPortal
Positive regulation of peptidyl-serine phosphorylationIEA:Ensembl
Regulation of cellular response to oxidative stressIEA:Ensembl
Substantia nigra developmentManual Assertion Based On ExperimentHEP:UniProtKB
TORC2 signalingManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Cell membrane
Cytoplasmic vesicle
Nucleus

References

Ezine, E., Lebbe, C., & Dumaz, N. (2023). 60P MAPKAP1/SIN1: A promising therapeutic target in resistant BRAF-mutated melanoma. ESMO Open, 8(1).

Ezine, E., Lebbe, C., & Dumaz, N. (2023). Unmasking the tumourigenic role of SIN1/MAPKAP1 in the mTOR complex 2. Clinical and Translational Medicine, 13(10), e1464.

Lin, X., Cheng, L., Wan, Y., Yan, Y., Zhang, Z., Li, X., ... & Xu, M. (2023). Ang II Controls the Expression of Mapkap1 by miR-375 and Affects the Function of Islet β Cells. Endocrine, Metabolic & Immune Disorders Drug Targets, 23(9), 1186.

Gao, S., Lv, Q., Xu, F., Li, H., & Guo, X. (2022). LncRNA CASC9-1 facilitates cell malignant behaviors in cervical squamous cell carcinoma by targeting miR-383-5p to up-regulate MAPKAP1. Archives of Medical Research, 53(2), 138-146.

Liu, H., Zhang, D., Zhou, Y., & Cui, S. (2021). MicroRNA-7a inhibits Isl1 expression to regulate insulin secretion by targeting Raf1 and Mapkap1 in NIT-1 cells. In Vitro Cellular & Developmental Biology-Animal, 57, 817-824.

Huang, L., Xu, W., Yan, D., You, X., Shi, X., Zhang, S., ... & Dai, L. (2020). Genetic predisposition to glioma mediated by a MAPKAP1 enhancer variant. Cellular and Molecular Neurobiology, 40, 643-652.

Yang, C., Li, S., Ma, J. X., Li, Y., Zhang, A., Sun, N., ... & Zhang, K. (2019). Whole exome sequencing identifies a novel predisposing gene, MAPKAP1, for familial mixed mood disorder. Frontiers in genetics, 10, 74.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-MAPKAP1 Recombinant Antibody (CBXS-1919)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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