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Mouse Anti-MAPKAPK5 Recombinant Antibody (13H5) (CBMAB-P0496-YC)

Provided herein is a Mouse monoclonal antibody against Human Mitogen-Activated Protein Kinase-Activated Protein Kinase 5. The antibody can be used for immunoassay techniques, such as ELISA, ICC, IF, WB.
See all MAPKAPK5 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
13H5
Antibody Isotype
IgG1
Application
ELISA, ICC, IF, WB

Basic Information

Immunogen
Recombinant human protein purified from E. coli (His-PRAK)
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
HEPES with 0.01% BSA, 150 mM sodium chloride, 50% glycerol and 0.03% sodium azide
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Mitogen-Activated Protein Kinase-Activated Protein Kinase 5
Introduction
MAPKAPK5 is a tumor suppressor and member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. The encoded protein is found in the nucleus but translocates to the cytoplasm upon phosphorylation and activation. This kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported.
Entrez Gene ID
Human8550
Mouse17165
Rat498183
UniProt ID
HumanQ8IW41
MouseO54992
RatF1LN87
Alternative Names
Mitogen-Activated Protein Kinase-Activated Protein Kinase 5; P38-Regulated/Activated Protein Kinase; MAPK-Activated Protein Kinase 5; MAPKAP Kinase 5; EC 2.7.11.1; MAPKAP-K5; MAPKAPK-5;
Function
Tumor suppressor serine/threonine-protein kinase involved in mTORC1 signaling and post-transcriptional regulation. Phosphorylates FOXO3, ERK3/MAPK6, ERK4/MAPK4, HSP27/HSPB1, p53/TP53 and RHEB. Acts as a tumor suppressor by mediating Ras-induced senescence and phosphorylating p53/TP53. Involved in post-transcriptional regulation of MYC by mediating phosphorylation of FOXO3: phosphorylation of FOXO3 leads to promote nuclear localization of FOXO3, enabling expression of miR-34b and miR-34c, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent MYC translation. Acts as a negative regulator of mTORC1 signaling by mediating phosphorylation and inhibition of RHEB. Part of the atypical MAPK signaling via its interaction with ERK3/MAPK6 or ERK4/MAPK4: the precise role of the complex formed with ERK3/MAPK6 or ERK4/MAPK4 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPK (ERK3/MAPK6 or ERK4/MAPK4), ERK3/MAPK6 (or ERK4/MAPK4) is phosphorylated and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6 (or ERK4/MAPK4). Mediates phosphorylation of HSP27/HSPB1 in response to PKA/PRKACA stimulation, inducing F-actin rearrangement.
Biological Process
Cellular senescenceTAS:Reactome
Intracellular signal transductionManual Assertion Based On ExperimentIBA:GO_Central
Negative regulation of TOR signalingISS:UniProtKB
Peptidyl-serine phosphorylationManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of telomerase activityManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of telomere cappingManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of telomere maintenance via telomeraseManual Assertion Based On ExperimentIMP:BHF-UCL
Protein autophosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Ras protein signal transductionManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of signal transduction by p53 class mediatorTAS:Reactome
Regulation of translationManual Assertion Based On ExperimentIDA:UniProtKB
Signal transductionManual Assertion Based On ExperimentTAS:ProtInc
Stress-induced premature senescenceManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Cytoplasm
Nucleus
Translocates to the cytoplasm following phosphorylation and activation. Interaction with ERK3/MAPK6 or ERK4/MAPK4 and phosphorylation at Thr-182, activates the protein kinase activity, followed by translocation to the cytoplasm. Phosphorylation by PKA/PRKACA at Ser-115 also induces nuclear export.
PTM
Phosphorylated on Thr-182 ERK3/MAPK6 or ERK4/MAPK4; which is the regulatory phosphorylation site and is located on the T-loop/loop 12, leading to activation. Phosphorylation at Thr-182 by p38-alpha/MAPK14, p38-beta/MAPK11 is subject to debate. Phosphorylated at Ser-115 by PKA/PRKACA, leading to localization to the cytoplasm. Autophosphorylated (By similarity).

Miao, J., Gao, Y., Guan, W., Yu, X., Wang, Y., Jiang, P., ... & You, W. (2023). High level of LncRNA MAPKAPK5-AS1 predicts poor prognosis and contributes to the malignant proliferation and EMT of non-small cell lung cancer via sponging miR-490-3p from HMGB2. Genes & Genomics, 45(5), 611-625.

Zhao, Y., Zhou, D., Yuan, Y., Chen, Y., Zhang, K., Tan, Y., & Fang, S. (2023). MAPKAPK5-AS1/miR-515-5p/CAB39 Axis contributes to non-small cell lung cancer cell proliferation and migration. Molecular Biotechnology, 1-11.

Maroofian, R., Efthymiou, S., Suri, M., Rahman, F., Zaki, M. S., Maqbool, S., ... & Houlden, H. (2023). Consolidating the association of biallelic MAPKAPK5 pathogenic variants with a distinct syndromic neurodevelopmental disorder. Journal of Medical Genetics, 60(8), 791-796.

Vecchio, D., Cocciadiferro, D., Macchiaiolo, M., Gonfiantini, M. V., Agolini, E., Matraxia, M., ... & Bartuli, A. (2022). Expanding the novel MAPKAPK5–related developmental disorder's genotype–phenotype correlation: Patient report and 19 months of follow‐up. Clinical Genetics, 102(2), 142-148.

Peng, Z., Ouyang, X., Wang, Y., & Fan, Q. (2022). MAPKAPK5-AS1 drives the progression of hepatocellular carcinoma via regulating miR-429/ZEB1 axis. BMC Molecular and Cell Biology, 23(1), 1-11.

Wang, L., Sun, L., Liu, R., Mo, H., Niu, Y., Chen, T., ... & Liu, Q. (2021). Long non-coding RNA MAPKAPK5-AS1/PLAGL2/HIF-1α signaling loop promotes hepatocellular carcinoma progression. Journal of Experimental & Clinical Cancer Research, 40(1), 1-18.

Horn, D., Fernández-Núñez, E., Gomez-Carmona, R., Rivera-Barahona, A., Nevado, J., Schwartzmann, S., ... & Ruiz-Perez, V. L. (2021). Biallelic truncating variants in MAPKAPK5 cause a new developmental disorder involving neurological, cardiac, and facial anomalies combined with synpolydactyly. Genetics in Medicine, 23(4), 679-688.

Chen, W., Gao, G., Yan, M., Yu, M., Shi, K., & Yang, P. (2021). Long noncoding RNA MAPKAPK5-AS1 promoted lipopolysaccharide-induced inflammatory damage in the myocardium by sponging microRNA-124-3p/E2F3. Molecular Medicine, 27(1), 1-14.

Zhou, Y., Liu, S., Luo, Y., Zhang, M., Jiang, X., & Xiong, Y. (2020). IncRNA MAPKAPK5-AS1 promotes proliferation and migration of thyroid cancer cell lines by targeting miR-519e-5p/YWHAH. European journal of histochemistry: EJH, 64(4).

Ji, H., Hui, B., Wang, J., Zhu, Y., Tang, L., Peng, P., ... & Wang, K. (2019). Long noncoding RNA MAPKAPK5‐AS1 promotes colorectal cancer proliferation by partly silencing p21 expression. Cancer Science, 110(1), 72-85.

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For research use only. Not intended for any clinical use.

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