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Mouse Anti-MARCKSL1 Recombinant Antibody (2H5) (CBMAB-A5304-LY)

The product is antibody recognizes MARCKSL1. The antibody 2H5 immunoassay techniques such as: WB, ELISA.
See all MARCKSL1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2H5
Antibody Isotype
IgG2b, κ
Application
WB, ELISA

Basic Information

Immunogen
MARCKSL1 (NP_075385, 107 a.a. ~ 195 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
MARCKS Like 1
Entrez Gene ID
65108
UniProt ID
P49006
Alternative Names
F52; MACMARCKS; MLP; MLP1; MRP
Function
Controls cell movement by regulating actin cytoskeleton homeostasis and filopodium and lamellipodium formation (PubMed:22751924).

When unphosphorylated, induces cell migration (By similarity).

When phosphorylated by MAPK8, induces actin bundles formation and stabilization, thereby reducing actin plasticity, hence restricting cell movement, including neuronal migration (By similarity).

May be involved in coupling the protein kinase C and calmodulin signal transduction systems (By similarity).
Biological Process
Actin filament organization Source: GO_Central
Central nervous system development Source: GO_Central
Positive regulation of cell population proliferation Source: Ensembl
Cellular Location
Plasma membrane
Cell membrane
Cytoskeleton
Note: Associates with the membrane via the insertion of the N-terminal N-myristoyl chain and the partial insertion of the effector domain. Association of the effector domain with membranes may be regulated by Ca2+/calmodulin. Colocalizes with F-actin at the leading edge of migrating cells (By similarity). In prostate cancers, shows strong expression at apical and/or basal regions of the cell and also has weak cytoplasmic expression (PubMed:22751924).
PTM
Phosphorylated. Phosphorylation at Ser-120 and Thr-178 is non-redundantly catalyzed by MAPK8 in vivo. Phosphorylation at Thr-148 is preferentially catalyzed by MAPK8 in vivo, but this modification can also be catalyzed by other kinases in the absence of MAPK8. May be phosphorylated by protein kinase C, which disrupts the interaction with calmodulin.

Zhao, Y., Xie, X., Tian, L., Liu, F., Sun, Y., Lu, H., ... & Mao, Y. (2023). MARCKSL1 interacted with F‐actin to promote esophageal squamous cell carcinoma mobility by modulating the formation of invadopodia. Cancer Medicine, 12(3), 3299-3312.

Yadav, V., Jena, M. K., Parashar, G., Parashar, N. C., Joshi, H., Ramniwas, S., & Tuli, H. S. (2023). Emerging role of microRNAs as regulators of protein kinase C substrate MARCKS and MARCKSL1 in cancer. Experimental Cell Research, 113891.

Jiang, M., Qi, F., Zhang, K., Zhang, X., Ma, J., Xia, S., ... & Chen, D. (2022). MARCKSL1–2 reverses docetaxel-resistance of lung adenocarcinoma cells by recruiting SUZ12 to suppress HDAC1 and elevate miR-200b. Molecular Cancer, 21(1), 1-16.

Li, F., Zhao, J., Wang, L., Chi, Y., Huang, X., & Liu, W. (2022). METTL14-mediated miR-30c-1-3p maturation represses the progression of lung cancer via regulation of MARCKSL1 expression. Molecular Biotechnology, 1-14.

Liang, W., Gao, R., Yang, M., Wang, X., Cheng, K., Shi, X., ... & Yu, X. (2020). MARCKSL1 promotes the proliferation, migration and invasion of lung adenocarcinoma cells. Oncology Letters, 19(3), 2272-2280.

Kondrychyn, I., Kelly, D. J., Carretero, N. T., Nomori, A., Kato, K., Chong, J., ... & Phng, L. K. (2020). Marcksl1 modulates endothelial cell mechanoresponse to haemodynamic forces to control blood vessel shape and size. Nature Communications, 11(1), 5476.

Chanda, S., Ang, C. E., Lee, Q. Y., Ghebrial, M., Haag, D., Shibuya, Y., ... & Südhof, T. C. (2019). Direct reprogramming of human neurons identifies MARCKSL1 as a pathogenic mediator of valproic acid-induced teratogenicity. Cell stem cell, 25(1), 103-119.

Egeland, N. G., Austdal, M., van Diermen-Hidle, B., Rewcastle, E., Gudlaugsson, E. G., Baak, J. P., ... & Jonsdottir, K. (2019). Validation study of MARCKSL1 as a prognostic factor in lymph node-negative breast cancer patients. Plos one, 14(3), e0212527.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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