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Mouse Anti-MARK4 (AA 390-467 ) Recombinant Antibody (CBFYM-1727) (CBMAB-M1894-FY)

This product is mouse antibody that recognizes MARK4. The antibody CBFYM-1727 can be used for immunoassay techniques such as: WB, IHC.
See all MARK4 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYM-1727
Antibody Isotype
IgG1
Application
WB, IHC

Basic Information

Immunogen
Human recombinant protein fragment corresponding to amino acids 390-467 of human MARK4 (NP_113605) produced in E.coli
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 390-467

Target

Full Name
MICROTUBULE AFFINITY REGULATING KINASE 4
Introduction
This gene encodes a member of the microtubule affinity-regulating kinase family. These protein kinases phosphorylate microtubule-associated proteins and regulate the transition between stable and dynamic microtubules. The encoded protein is associated with the centrosome throughout mitosis and may be involved in cell cycle control. Expression of this gene is a potential marker for cancer, and the encoded protein may also play a role in Alzheimer's disease. Pseudogenes of this gene are located on both the short and long arm of chromosome 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Entrez Gene ID
57787
UniProt ID
Q96L34
Alternative Names
Microtubule Affinity Regulating Kinase 4; MAP/Microtubule Affinity-Regulating Kinase Like 1; MAP/Microtubule Affinity-Regulating Kinase 4; EC 2.7.11.1; MARKL1; MAP/Microtubule Affinity-Regulating Kinase-Like 1; MARK4 Serine/Threonine Protein Kinase
Function
Serine/threonine-protein kinase (PubMed:15009667, PubMed:14594945, PubMed:23666762, PubMed:23184942).

Phosphorylates the microtubule-associated protein MAPT/TAU (PubMed:14594945, PubMed:23666762).

Also phosphorylates the microtubule-associated proteins MAP2 and MAP4 (PubMed:14594945).

Involved in regulation of the microtubule network, causing reorganization of microtubules into bundles (PubMed:14594945, PubMed:25123532).

Required for the initiation of axoneme extension during cilium assembly (PubMed:23400999).

Regulates the centrosomal location of ODF2 and phosphorylates ODF2 in vitro (PubMed:23400999).

Plays a role in cell cycle progression, specifically in the G1/S checkpoint (PubMed:25123532).

Reduces neuronal cell survival (PubMed:15009667).

Plays a role in energy homeostasis by regulating satiety and metabolic rate (By similarity).

Promotes adipogenesis by activating JNK1 and inhibiting the p38MAPK pathway, and triggers apoptosis by activating the JNK1 pathway (By similarity).

Phosphorylates mTORC1 complex member RPTOR and acts as a negative regulator of the mTORC1 complex, probably due to disruption of the interaction between phosphorylated RPTOR and the RRAGA/RRAGC heterodimer which is required for mTORC1 activation (PubMed:23184942).
Biological Process
Cell cycle Source: UniProtKB-KW
Cell division Source: UniProtKB-KW
Cilium organization Source: ARUK-UCL
Intracellular signal transduction Source: GO_Central
Microtubule bundle formation Source: UniProtKB
Microtubule cytoskeleton organization Source: UniProtKB
Nervous system development Source: UniProtKB
Positive regulation of cell cycle Source: ARUK-UCL
Positive regulation of cilium assembly Source: ARUK-UCL
Positive regulation of programmed cell death Source: UniProtKB
Positive regulation of protein localization to centrosome Source: ARUK-UCL
Protein phosphorylation Source: UniProtKB
Regulation of centrosome cycle Source: ARUK-UCL
Cellular Location
Cytoskeleton
Centrosome
Microtubule organizing center
Cilium basal body
Cilium axoneme
Cytoplasm and Cytosol
Cytoplasm
Other locations
Dendrite
Note: Localized at the tips of neurite-like processes in differentiated neuroblast cells. Detected in the cytoplasm and neuropil of the hippocampus.
PTM
Ubiquitinated with 'Lys-29'- and 'Lys-33'-linked polyubiquitins which appear to impede LKB1-mediated phosphorylation. Deubiquitinated by USP9X.
Phosphorylated at Thr-214 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39 (PubMed:14976552). Phosphorylated throughout the cell cycle (PubMed:25123532).

Anwar, S., Khan, S., Anjum, F., Shamsi, A., Khan, P., Fatima, H., ... & Hassan, M. I. (2022). Myricetin inhibits breast and lung cancer cells proliferation via inhibiting MARK4. Journal of Cellular Biochemistry, 123(2), 359-374.

Shamsi, A., DasGupta, D., Alhumaydhi, F. A., Khan, M. S., Alsagaby, S. A., Al Abdulmonem, W., ... & Yadav, D. K. (2022). Inhibition of MARK4 by serotonin as an attractive therapeutic approach to combat Alzheimer's disease and neuroinflammation. RSC Medicinal Chemistry, 13(6), 737-745.

Yu, X., Chen, X., Amrute-Nayak, M., Allgeyer, E., Zhao, A., Chenoweth, H., ... & Li, X. (2021). MARK4 controls ischaemic heart failure through microtubule detyrosination. Nature, 594(7864), 560-565.

Anwar, S., Khan, S., Shamsi, A., Anjum, F., Shafie, A., Islam, A., ... & Hassan, M. I. (2021). Structure‐based investigation of MARK4 inhibitory potential of Naringenin for therapeutic management of cancer and neurodegenerative diseases. Journal of cellular biochemistry, 122(10), 1445-1459.

Anwar, S., Shamsi, A., Shahbaaz, M., Queen, A., Khan, P., Hasan, G. M., ... & Hassan, M. I. (2020). Rosmarinic acid exhibits anticancer effects via MARK4 inhibition. Scientific Reports, 10(1), 10300.

Anwar, S., Shamsi, A., Kar, R. K., Queen, A., Islam, A., Ahmad, F., & Hassan, M. I. (2020). Structural and biochemical investigation of MARK4 inhibitory potential of cholic acid: Towards therapeutic implications in neurodegenerative diseases. International Journal of Biological Macromolecules, 161, 596-604.

Li, F., Liu, Z., Sun, H., Li, C., Wang, W., Ye, L., ... & Wang, H. (2020). PCC0208017, a novel small-molecule inhibitor of MARK3/MARK4, suppresses glioma progression in vitro and in vivo. Acta Pharmaceutica Sinica B, 10(2), 289-300.

Shamsi, A., Anwar, S., Mohammad, T., Alajmi, M. F., Hussain, A., Rehman, M. T., ... & Hassan, M. I. (2020). MARK4 inhibited by AChE inhibitors, donepezil and Rivastigmine tartrate: Insights into Alzheimer’s disease therapy. Biomolecules, 10(5), 789.

Qiao, K., Ning, S., Wan, L., Wu, H., Wang, Q., Zhang, X., ... & Pang, D. (2019). LINC00673 is activated by YY1 and promotes the proliferation of breast cancer cells via the miR-515-5p/MARK4/Hippo signaling pathway. Journal of Experimental & Clinical Cancer Research, 38, 1-15.

Clement, M., Chen, X., Chenoweth, H. L., Teng, Z., Thome, S., Newland, S. A., ... & Li, X. (2019). MARK4 (microtubule affinity-regulating kinase 4)-dependent inflammasome activation promotes atherosclerosis—brief report. Arteriosclerosis, thrombosis, and vascular biology, 39(8), 1645-1651.

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For research use only. Not intended for any clinical use.

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