Mouse Anti-MBD4 Recombinant Antibody (CBFYM-1804) (CBMAB-M1972-FY)

This product is mouse antibody that recognizes MBD4. The antibody CBFYM-1804 can be used for immunoassay techniques such as: ELISA, WB.
See all MBD4 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBFYM-1804

Application

ELISA, WB

Basic Information

Specificity

Human

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

METHYL-CPG BINDING DOMAIN 4, DNA GLYCOSYLASE

Introduction

The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain. These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains an MBD domain at the N-terminus that functions both in binding to methylated DNA and in protein interactions and a C-terminal mismatch-specific glycosylase domain that is involved in DNA repair. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Entrez Gene ID

8930

UniProt ID

O95243

Alternative Names

Methyl-CpG Binding Domain 4, DNA Glycosylase; Methyl-CpG Binding Domain Protein 4; Mismatch-Specific DNA N-Glycosylase; Methyl-CpG-Binding Endonuclease 1; Methyl-CpG-Binding Protein MBD4; MED1; G/5-Fluorouracil Mismatch Glycosylase With Biphasic Kinetics

Function

Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein.

Biological Process

Depyrimidination Source: Reactome
DNA repair Source: ProtInc

Cellular Location

Nucleus

References

Han, Y., Wang, Y., Zhang, C., Li, Y., Guo, J., & Tian, C. (2023). Metastasis-associated lung adenocarcinoma transcript 1 induces methyl-CpG-binding domain protein 4 in mice with recurrent spontaneous abortion caused by anti-phospholipid antibody positivity. Placenta, 137, 38-48.

Papin, C., Ibrahim, A., Sabir, J. S., Le Gras, S., Stoll, I., Albiheyri, R. S., ... & Hamiche, A. (2023). MBD4 loss results in global reactivation of promoters and retroelements with low methylated CpG density. Journal of experimental & clinical cancer research, 42(1), 301.

Ouzon-Shubeita, H., Schmaltz, L. F., & Lee, S. (2021). Insights into the substrate discrimination mechanisms of methyl-CpG-binding domain 4. Biochemical Journal, 478(10), 1985-1997.

Song, X., Zhang, Y., Zhong, Q., Zhan, K., Bi, J., Tang, J., ... & Li, B. (2020). Identification and functional characterization of methyl-CpG binding domain protein from Tribolium castaneum. Genomics, 112(3), 2223-2232.

Ouzon-Shubeita, H., Jung, H., Lee, M. H., Koag, M. C., & Lee, S. (2020). Catalytic mechanism of the mismatch-specific DNA glycosylase methyl-CpG-binding domain 4. Biochemical Journal, 477(9), 1601-1612.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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