Mouse Anti-MCFD2 Recombinant Antibody (X1) (CBMAB-A5342-LY)

Name: Mouse Anti-MCFD2 Recombinant Antibody (X1)
SKU: CBMAB-A5342-LY
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

Antibody Isotype

IgG1, κ

Application

WB, ELISA

Basic Information

Immunogen

MCFD2 (AAH40357, 1 a.a. ~ 146 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

multiple coagulation factor deficiency 2

Entrez Gene ID

90411

UniProt ID

Q8NI22

Alternative Names

DKFZp686G21263; F5F8D; LMAN1IP; SDNSF

Function

The MCFD2-LMAN1 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins. Plays a role in the secretion of coagulation factors.

Biological Process

Protein transport Source: UniProtKB-KW
Vesicle-mediated transport Source: UniProtKB-KW

Cellular Location

Endoplasmic reticulum
Golgi apparatus
Other locations
Endoplasmic reticulum-Golgi intermediate compartment

Involvement in disease

Factor V and factor VIII combined deficiency 2 (F5F8D2):
A blood coagulation disorder characterized by bleeding symptoms similar to those in hemophilia or parahemophilia, that are caused by single deficiency of FV or FVIII, respectively. The most common symptoms are epistaxis, menorrhagia, and excessive bleeding during or after trauma. Plasma levels of coagulation factors V and VIII are in the range of 5 to 30% of normal.

More Infomation

References

Watanabe, S., Kise, Y., Yonezawa, K., Inoue, M., Shimizu, N., Nureki, O., & Inaba, K. (2023). Structure of full-length ERGIC-53 in complex with MCFD2 for cargo transport. bioRxiv, 2023-08.

Zhang, Y., Liu, Z., & Zhang, B. (2023). Separate roles of LMAN1 and MCFD2 in ER-to-Golgi trafficking of FV and FVIII. Blood Advances, 7(7), 1286-1296.

Kirimoto, Y., Yamano-Adachi, N., Koga, Y., & Omasa, T. (2023). Effect of co-overexpression of the cargo receptor ERGIC-53/MCFD2 on antibody production and intracellular IgG secretion in recombinant Chinese hamster ovary cells. Journal of bioscience and bioengineering, 136(5), 400-406.

Zhang, Y., Zhu, M., Zheng, C., Wei, W., Emmer, B. T., & Zhang, B. (2022). LMAN1–MCFD2 complex is a cargo receptor for the ER-Golgi transport of α1-antitrypsin. Biochemical Journal, 479(7), 839-855.

Zhang, Y., Liu, Z., & Zhang, B. (2022). Separate roles of LMAN1 and MCFD2 in ER-to-Golgi trafficking of factor V and factor VIII. bioRxiv, 2022-09.

Satoh, T., Nishio, M., Suzuki, K., Yagi-Utsumi, M., Kamiya, Y., Mizushima, T., & Kato, K. (2020). Crystallographic snapshots of the EF-hand protein MCFD2 complexed with the intracellular lectin ERGIC-53 involved in glycoprotein transport. Acta Crystallographica Section F: Structural Biology Communications, 76(5), 216-221.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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