Rat Anti-MDC1 (AA 1-335) Recombinant Antibody (CBFYM-1934) (CBMAB-M2108-FY)

Name: Rat Anti-MDC1 (AA 1-335) Recombinant Antibody (CBFYM-1934)
SKU: CBMAB-M2108-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rat

Clone

CBFYM-1934

Application

Immunogen

E.coli-derived recombinant human MDC1, Met1-Glu335

Specificity

Human

Antibody Isotype

IgG2a

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS

Concentration

LYOPH

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 1-335

More Infomation

Target

Full Name

Mediator Of DNA Damage Checkpoint 1

Introduction

The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci.

Entrez Gene ID

9656

UniProt ID

Q14676

Alternative Names

Mediator Of DNA Damage Checkpoint 1; Nuclear Factor With BRCT Domains 1; NFBD1; Homologue To Drosophila Photoreceptor Protein Calphotin; Mediator Of DNA Damage Checkpoint Protein 1; Mediator Of DNA-Damage Checkpoint 1; KIAA0170

Function

Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1 and CHEK2 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1.

Biological Process

DNA repair Source: UniProtKB-KW
Mitotic intra-S DNA damage checkpoint signaling Source: UniProtKB

Cellular Location

Nucleus
Other locations
Chromosome
Note: Associated with chromatin. Relocalizes to discrete nuclear foci following DNA damage, this requires 'Ser-139' phosphorylation of H2AX. Colocalizes with APTX at sites of DNA double-strand breaks.

PTM

Phosphorylated upon exposure to ionizing radiation (IR), ultraviolet radiation (UV), and hydroxyurea (HU). Phosphorylation in response to IR requires ATM, NBN, and possibly CHEK2. Also phosphorylated during the G2/M phase of the cell cycle and during activation of the mitotic spindle checkpoint. Phosphorylation at Thr-4 by ATM stabilizes and enhances homodimerization via the FHA domain.
Sumoylation at Lys-1840 by PIAS4 following DNA damage promotes ubiquitin-mediated degradation.
Ubiquitinated by RNF4, leading to proteasomal degradation; undergoes 'Lys-48'-linked polyubiquitination.

Sottnik, J. L., Shackleford, M. T., Richer, A. L., Fu, R., Hesselberth, J. R., & Sikora, M. J. (2023). Co-regulator activity of Mediator of DNA Damage Checkpoint 1 (MDC1) is associated with DNA repair dysfunction and PARP inhibitor sensitivity in lobular carcinoma of the breast. bioRxiv, 2023-10.

Choi, S. H., Cho, K., Kim, E. S., & Yoo, H. Y. (2022). Proline-serine-threonine-repeat region of MDC1 mediates Chk1 phosphorylation and the DNA double-strand break repair. The International Journal of Biochemistry & Cell Biology, 143, 106152.

Chou, J., Kaller, M., Jaeckel, S., Rokavec, M., & Hermeking, H. (2022). AP4 suppresses DNA damage, chromosomal instability and senescence via inducing MDC1/Mediator of DNA damage Checkpoint 1 and repressing MIR22HG/miR-22-3p. Molecular Cancer, 21(1), 1-24.

Xie, R., Yan, Z., Jing, J., Wang, Y., Zhang, J., Li, Y., ... & Wu, C. (2022). Functional defects of cancer-associated MDC1 mutations in DNA damage repair. DNA repair, 114, 103330.

Sottnik, J. L., Bordeaux, E. K., Mehrotra, S., Ferrara, S. E., Goodspeed, A. E., Costello, J. C., & Sikora, M. J. (2021). Mediator of DNA damage checkpoint 1 (MDC1) is a novel estrogen receptor coregulator in invasive lobular carcinoma of the breast. Molecular Cancer Research, 19(8), 1270-1282.

Ruff, S. E., Logan, S. K., Garabedian, M. J., & Huang, T. T. (2020). Roles for MDC1 in cancer development and treatment. DNA repair, 95, 102948.

Zhang, X., Hu, F., Liu, L., & Xu, B. (2019). Effect of silencing of mediator of DNA damage checkpoint protein 1 on the growth of oral squamous cell carcinoma in vitro and in vivo. European Journal of Oral Sciences, 127(6), 494-499.

Beck, C., Castañeda-Zegarra, S., Huse, C., Xing, M., & Oksenych, V. (2019). Mediator of DNA damage checkpoint protein 1 facilitates V (D) J recombination in cells lacking DNA repair factor XLF. Biomolecules, 10(1), 60.

Salguero, I., Belotserkovskaya, R., Coates, J., Sczaniecka-Clift, M., Demir, M., Jhujh, S., ... & Jackson, S. P. (2019). MDC1 PST-repeat region promotes histone H2AX-independent chromatin association and DNA damage tolerance. Nature Communications, 10(1), 5191.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
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For research use only. Not intended for any clinical use.

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