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Mouse Anti-MDFIC Recombinant Antibody (CBFYM-1937) (CBMAB-M2111-FY)

This product is mouse antibody that recognizes MDFIC. The antibody CBFYM-1937 can be used for immunoassay techniques such as: WB, IP, IF, ELISA.
See all MDFIC antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYM-1937
Application
WB, IP, IF, ELISA

Basic Information

Specificity
Human
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
MYOD FAMILY INHIBITOR DOMAIN CONTAINING
Introduction
This gene product is a member of a family of proteins characterized by a specific cysteine-rich C-terminal domain, which is involved in transcriptional regulation of viral genome expression. Alternative translation initiation from an upstream non-AUG, and an in-frame, downstream AUG codon, results in the production of two isoforms, p40 and p32, respectively, which have different subcellular localization; p32 is mainly found in the cytoplasm, whereas p40 is targeted to the nucleolus. Both isoforms have transcriptional regulatory activity that is attributable to the cysteine-rich C-terminal domain. Alternative splicing results in multiple transcript variants.
Entrez Gene ID
29969
UniProt ID
Q9P1T7
Alternative Names
MyoD Family Inhibitor Domain Containing; I-Mfa Domain-Containing Protein; HIC; MyoD Family Inhibitor Domain-Containing Protein; MDFIC1
Function
Acts as a transcriptional activator or repressor. Inhibits the transcriptional activation of Zic family proteins ZIC1, ZIC2 and ZIC3. Retains nuclear Zic proteins ZIC1, ZIC2 and ZIC3 in the cytoplasm. Modulates the expression from both cellular and viral promoters. Down-regulates Tat-dependent transcription of the human immunodeficiency virus type 1 (HIV-1) LTR by interacting with HIV-1 Tat and Rev and impairing their nuclear import, probably by rendering the NLS domains inaccessible to importin-beta. Also stimulates activation of human T-cell leukemia virus type I (HTLV-I) LTR. Binds to the axin complex, resulting in an increase in the level of free beta-catenin. Affects axin regulation of the WNT and JNK signaling pathways.
Biological Process
Negative regulation of protein import into nucleus Source: UniProtKB
Negative regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of viral transcription Source: UniProtKB
Regulation of JNK cascade Source: UniProtKB
Regulation of Wnt signaling pathway Source: UniProtKB
Cellular Location
Isoform 1:
Nucleus
nucleolus
Note: Also shows a granular distribution in the cytoplasm.
Isoform 2:
Cytoplasm
Note: Weak expression in the nucleus.

Montazaribarforoushi, S. (2023). Defining The Role of a Novel Gene “MyoD Family Inhibitor Domain Containing (MDFIC)” Important in Cardiovascular Development (Doctoral dissertation).

Byrne, A. B., Brouillard, P., Sutton, D. L., Kazenwadel, J., Montazaribarforoushi, S., Secker, G. A., ... & Harvey, N. L. (2022). Pathogenic variants in MDFIC cause recessive central conducting lymphatic anomaly with lymphedema. Science translational medicine, 14(634), eabm4869.

Zhang, K., Liu, Z., Wang, Z., Zhou, Z., Shao, X., Hua, X., ... & Chen, K. (2021). Long non-coding RNA MDFIC-7 promotes chordoma progression through modulating the miR-525-5p/ARF6 axis. Frontiers in Oncology, 11, 743718.

Hu, X., Xing, Y., Ren, L., Wang, Y., Li, Q., Yang, Q., ... & Zhang, L. (2020). bta-miR-23a Regulates the Myogenic Differentiation of Fetal Bovine Skeletal Muscle-Derived Progenitor Cells by Targeting MDFIC Gene. Genes, 11(10), 1232.

Sui, Y., Li, X., Oh, S., Zhang, B., Freeman, W. M., Shin, S., & Janknecht, R. (2020). Opposite roles of the JMJD1A interaction partners MDFI and MDFIC in colorectal cancer. Scientific Reports, 10(1), 8710.

Cusick, J. K., Alhomsy, Y., Wong, S., Talbott, G., Uversky, V. N., Hart, C., ... & Shi, Y. (2020). RELT stains prominently in B-cell lymphomas and binds the hematopoietic transcription factor MDFIC. Biochemistry and Biophysics Reports, 24, 100868.

Huang, W., Guo, L., Zhao, M., Zhang, D., Xu, H., & Nie, Q. (2019). The inhibition on MDFIC and PI3K/AKT pathway caused by miR-146b-3p triggers suppression of myoblast proliferation and differentiation and promotion of apoptosis. Cells, 8(7), 656.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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