Mouse Anti-MED10 Recombinant Antibody (CBWJN-1137) (CBMAB-N0956-WJ)

Name: Mouse Anti-MED10 Recombinant Antibody (CBWJN-1137)
SKU: CBMAB-N0956-WJ
Rating: 5 (1 reviews)

See all MED10 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBWJN-1137

Application

ELISA, WB

Specificity

Human

Antibody Isotype

IgG1

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.2

Concentration

1 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Mediator Complex Subunit 10

Introduction

MED10 is a component of the Mediator complex, which is a coactivator for DNA-binding factors that activate transcription via RNA polymerase II (Sato et al., 2003 [PubMed 12584197]).[supplied by OMIM, Oct 2008]

Entrez Gene ID

84246

UniProt ID

Q9BTT4

Alternative Names

Mediator Complex Subunit 10; Transformation-Related Gene 17 Protein; Transformation-Related Gene 20 Protein; TRG-17; TRG-20; Mediator Of RNA Polymerase II Transcription, Subunit 10 Homolog (NUT2, S. Cerevisiae); Mediator Of RNA Polymerase II Transcription, Subunit 10;

Function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Biological Process

Positive regulation of transcription by RNA polymerase II Source: GO_Central
Positive regulation of transcription elongation from RNA polymerase II promoter Source: ComplexPortal
Positive regulation of transcription initiation from RNA polymerase II promoter Source: ComplexPortal
RNA polymerase II preinitiation complex assembly Source: ComplexPortal

Cellular Location

Nucleus

Bhardwaj, R., Thakur, J. K., & Kumar, S. (2021). MedProDB: A database of Mediator proteins. Computational and Structural Biotechnology Journal, 19, 4165-4176.

Zhao, H., Young, N., Kalchschmidt, J., Lieberman, J., El Khattabi, L., Casellas, R., & Asturias, F. J. (2021). Structure of mammalian Mediator complex reveals Tail module architecture and interaction with a conserved core. Nature communications, 12(1), 1355.

Maji, S., Dahiya, P., Waseem, M., Dwivedi, N., Bhat, D. S., Dar, T. H., & Thakur, J. K. (2019). Interaction map of Arabidopsis Mediator complex expounding its topology. Nucleic Acids Research, 47(8), 3904-3920.

Verger, A., Monté, D., & Villeret, V. (2019). Twenty years of Mediator complex structural studies. Biochemical Society Transactions, 47(1), 399-410.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-MED10 Recombinant Antibody (CBWJN-1137)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry