Mouse Anti-MED13 Recombinant Antibody (CBYJT-1462) (CBMAB-T0493-YJ)

Name: Mouse Anti-MED13 Recombinant Antibody (CBYJT-1462)
SKU: CBMAB-T0493-YJ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYJT-1462

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 476-775 mapping within an internal region of TRAP240 of human origin

Specificity

Human, Rat, Mouse

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH 7.4

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 476-775

More Infomation

Target

Full Name

Mediator Complex Subunit 13

Introduction

MED13 is a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. MED13 is proposed to form a sub-complex with MED12, cyclin C, and CDK8 that can negatively regulate transactivation by mediator.

Entrez Gene ID

Human	9969
Mouse	327987
Rat	303403

UniProt ID

Human	Q9UHV7
Mouse	Q5SWW4
Rat	F1LXU0

Alternative Names

Mediator Complex Subunit 13; Thyroid Hormone Receptor-Associated Protein Complex 240 KDa Component; Vitamin D3 Receptor-Interacting Protein Complex Component DRIP250; Activator-Recruited Cofactor 250 KDa Component; Thyroid Hormone Receptor-Associated Protein 1; DRIP250; TRAP240; THRAP1; ARC250

Function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Biological Process

Cholesterol homeostasis Source: Ensembl
Negative regulation of DNA-binding transcription factor activity Source: Ensembl
Positive regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: MGI
Positive regulation of transcription initiation from RNA polymerase II promoter Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: GO_Central
Triglyceride homeostasis Source: Ensembl

Cellular Location

Nucleus

Involvement in disease

Intellectual developmental disorder, autosomal dominant 61 (MRD61):
An autosomal dominant form of mental retardation, a disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior and manifested during the developmental period. MRD61 is characterized by global developmental delay apparent in infancy with mildly impaired intellectual development, expressive speech delay, and behavioral abnormalities, including autism spectrum disorder and attention deficit-hyperactivity disorder. Additional features are highly variable and may include non-specific dysmorphic features, obstipation, ocular anomalies, and poor overall growth.

Zhou, W., Cai, H., Li, J., Xu, H., Wang, X., Men, H., ... & Cai, L. (2021). Potential roles of mediator complex subunit 13 in cardiac diseases. International Journal of Biological Sciences, 17(1), 328.

Hamada, N., Iwamoto, I., Nishikawa, M., & Nagata, K. I. (2021). Expression analyses of mediator complex subunit 13-like: a responsible gene for neurodevelopmental disorders during mouse brain development. Developmental Neuroscience, 43(1), 43-52.

Roliński, M., Montaldo, N. P., Aksu, M. E., Fordyce Martin, S. L., Brambilla, A., Kunath, N., ... & van Loon, B. (2021). Loss of Mediator complex subunit 13 (MED13) promotes resistance to alkylation through cyclin D1 upregulation. Nucleic Acids Research, 49(3), 1470-1484.

Poot, M. (2019). Mutations in mediator complex genes CDK8, MED12, MED13, and MEDL13 mediate overlapping developmental syndromes. Molecular Syndromology, 10(5), 239-242.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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