Mouse Anti-MED18 Recombinant Antibody (CBFYM-2001) (CBMAB-M2179-FY)

This product is mouse antibody that recognizes MED18. The antibody CBFYM-2001 can be used for immunoassay techniques such as: WB, IP, IF, ELISA.
See all MED18 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBFYM-2001

Application

WB, IP, IF, ELISA

Basic Information

Specificity

Human

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

MEDIATOR COMPLEX SUBUNIT 18

Introduction

MED18 is a component of the Mediator complex, which is a coactivator for DNA-binding factors that activate transcription via RNA polymerase II.

Entrez Gene ID

54797

UniProt ID

Q9BUE0

Alternative Names

Mediator Complex Subunit 18; P28b; Mediator Of RNA Polymerase II Transcription, Subunit 18 Homolog (S. Cerevisiae); Mediator Of RNA Polymerase II Transcription, Subunit 18 Homolog; Mediator Of RNA Polymerase II Transcription Subunit 18; TRAP/Mediator Complex Subunit P28b; SRB5

Function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Biological Process

Positive regulation of transcription elongation from RNA polymerase II promoter Source: ComplexPortal
Positive regulation of transcription initiation from RNA polymerase II promoter Source: ComplexPortal
Regulation of transcription by RNA polymerase II Source: GO_Central
RNA polymerase II preinitiation complex assembly Source: ComplexPortal
Termination of RNA polymerase II transcription Source: GO_Central

Cellular Location

Nucleus

References

Prusty, A., Sharma, S., Malik, N., Kapoor, S., & Tyagi, A. K. (2023). Functional Versatility of Multi-Protein Mediator Complex in Plant Growth and Development. Critical Reviews in Plant Sciences, 1-39.

Morse, R. H. (2022). Function and dynamics of the Mediator complex: novel insights and new frontiers. Transcription, 13(1-3), 39-52.

Chen, J., Yang, S., Fan, B., Zhu, C., & Chen, Z. (2022). The mediator complex: a central coordinator of plant adaptive responses to environmental stresses. International Journal of Molecular Sciences, 23(11), 6170.

Sierecki, E. (2020, March). The Mediator complex and the role of protein-protein interactions in the gene regulation machinery. In Seminars in Cell & Developmental Biology (Vol. 99, pp. 20-30). Academic Press.

Crawford, T., Karamat, F., Lehotai, N., Rentoft, M., Blomberg, J., Strand, Å., & Björklund, S. (2020). Specific functions for Mediator complex subunits from different modules in the transcriptional response of Arabidopsis thaliana to abiotic stress. Scientific Reports, 10(1), 5073.

Verger, A., Monté, D., & Villeret, V. (2019). Twenty years of Mediator complex structural studies. Biochemical Society Transactions, 47(1), 399-410.

Maji, S., Dahiya, P., Waseem, M., Dwivedi, N., Bhat, D. S., Dar, T. H., & Thakur, J. K. (2019). Interaction map of Arabidopsis Mediator complex expounding its topology. Nucleic Acids Research, 47(8), 3904-3920.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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