Mouse Anti-MED20 Recombinant Antibody (CBYJT-1465) (CBMAB-T0496-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to MED20 (Mediator Complex Subunit 20). The antibody can be used for immunoassay techniques, such as WB, IP, IF, IHC-P, ELISA.
See all MED20 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Rat, Mouse

Clone

CBYJT-1465

Antibody Isotype

IgG

Application

WB, IP, IF, IHC-P, ELISA

Basic Information

Specificity

Human, Rat, Mouse

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH 7.4

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Mediator Complex Subunit 20

Introduction

MED20 is a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. A mutation in MED20 has been associated with a novel infantile-onset neurodegenerative movement disorder.

Entrez Gene ID

Human	9477
Mouse	56771
Rat	316209

UniProt ID

Human	Q9H944
Mouse	Q9R0X0
Rat	Q5XIE9

Alternative Names

Mediator Complex Subunit 20; TRFP; Trf (TATA Binding Protein-Related Factor)-Proximal Homolog (Drosophila); Trf (TATA Binding Protein-Related Factor)-Proximal Homolog; Mediator Of RNA Polymerase II Transcription Subunit 20; TRF-Proximal Protein Homolog; PRO0213; HTRFP; SRB2

Function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Biological Process

Positive regulation of transcription elongation from RNA polymerase II promoter Source: ComplexPortal
Positive regulation of transcription initiation from RNA polymerase II promoter Source: ComplexPortal
Regulation of transcription by RNA polymerase II Source: GO_Central
RNA polymerase II preinitiation complex assembly Source: ComplexPortal
Transcription, DNA-templated Source: UniProtKB
Transcription by RNA polymerase II Source: ProtInc

Cellular Location

Nucleus

References

Schiano, C., Luongo, L., Maione, S., & Napoli, C. (2023). Mediator complex in neurological disease. Life Sciences, 121986.

Morse, R. H. (2022). Function and dynamics of the Mediator complex: novel insights and new frontiers. Transcription, 13(1-3), 39-52.

Cevher, M. A. (2021). Reconstitution of Pol II (G) responsive form of the human Mediator complex. Turkish Journal of Biology, 45(3), 253-261.

Tang, W. S., Weng, L., Wang, X., Liu, C. Q., Hu, G. S., Yin, S. T., ... & Zhao, T. J. (2021). The Mediator subunit MED20 organizes the early adipogenic complex to promote development of adipose tissues and diet-induced obesity. Cell Reports, 36(1).

Guo, P., Chong, L., Wu, F., Hsu, C. C., Li, C., Zhu, J. K., & Zhu, Y. (2021). Mediator tail module subunits MED16 and MED25 differentially regulate abscisic acid signaling in Arabidopsis. Journal of Integrative Plant Biology, 63(4), 802-815.

Sierecki, E. (2020, March). The Mediator complex and the role of protein-protein interactions in the gene regulation machinery. In Seminars in Cell & Developmental Biology (Vol. 99, pp. 20-30). Academic Press.

Cui, W., Marcho, C., Wang, Y., Degani, R., Golan, M., Tremblay, K. D., ... & Mager, J. (2019). MED20 is essential for early embryogenesis and regulates NANOG expression. Reproduction, 157(3), 215-222.

Verger, A., Monté, D., & Villeret, V. (2019). Twenty years of Mediator complex structural studies. Biochemical Society Transactions, 47(1), 399-410.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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