Mouse Anti-MED22 Antibody (2A7) (CBMAB-0647-YC)

Provided herein are mouse monoclonal antibodies against Human MED22. The antibody clone 2A7 can be used for immunoassay techniques, such as ELISA and IP.
See all MED22 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

2A7

Antibody Isotype

IgG2b

Application

ELISA, IP

Basic Information

Immunogen

Recombinant protein

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Supernatant

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

MED22

Introduction

MED22 (mediator of RNA polymerase II transcription subunit 22) is a protein component of the mediator complex. MED22 functions in the regulation of transcription by bridging interactions between gene-specific regulatory factors, RNA polymerase II, and general transcription factors.

Entrez Gene ID

6837

UniProt ID

Q15528

Alternative Names

Med24; SURF5

Function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Biological Process

Positive regulation of transcription elongation from RNA polymerase II promoter Source: ComplexPortal
Positive regulation of transcription initiation from RNA polymerase II promoter Source: ComplexPortal
RNA polymerase II preinitiation complex assembly Source: ComplexPortal

Cellular Location

Nucleus

References

Rodriguez, P. Q., Unnersjö-Jess, D., Zambrano, S. S., Guo, J., Möller-Hackbarth, K., Blom, H., ... & Patrakka, J. (2020). Inactivation of mediator complex protein 22 in podocytes results in intracellular vacuole formation, podocyte loss and premature death. Scientific Reports, 10(1), 20037.

Maji, S., Dahiya, P., Waseem, M., Dwivedi, N., Bhat, D. S., Dar, T. H., & Thakur, J. K. (2019). Interaction map of Arabidopsis Mediator complex expounding its topology. Nucleic Acids Research, 47(8), 3904-3920.

Verger, A., Monté, D., & Villeret, V. (2019). Twenty years of Mediator complex structural studies. Biochemical Society Transactions, 47(1), 399-410.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-MED22 (AA 1-100) Recombinant Antibody (CBFYM-2003)

Mouse Anti-MED22 Recombinant Antibody (3A9)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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