Mouse Anti-MED23 Recombinant Antibody (4H6) (CBMAB-A1864-LY)

Name: Mouse Anti-MED23 Recombinant Antibody (4H6)
SKU: CBMAB-A1864-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

4H6

Application

sELISA, ELISA

Immunogen

CRSP3 (NP_004821, 531 a.a. ~ 630 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Mediator Complex Subunit 23

Introduction

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein also acts as a metastasis suppressor. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]

Entrez Gene ID

9439

UniProt ID

Q9ULK4

Alternative Names

CRSP130; CRSP133; CRSP3; DKFZp434H0117; DRIP130; SUR2

Function

Required for transcriptional activation subsequent to the assembly of the pre-initiation complex (By similarity).

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. Required for transcriptional activation by adenovirus E1A protein. Required for ELK1-dependent transcriptional activation in response to activated Ras signaling.

Biological Process

Positive regulation of gene expression Source: GO_Central
Positive regulation of transcription elongation from RNA polymerase II promoter Source: ComplexPortal
Positive regulation of transcription initiation from RNA polymerase II promoter Source: ComplexPortal
Regulation of transcription, DNA-templated Source: MGI
Regulation of transcription by RNA polymerase II Source: GO_Central
RNA polymerase II preinitiation complex assembly Source: ComplexPortal
Transcription initiation from RNA polymerase II promoter Source: ProtInc

Cellular Location

Nucleus

Involvement in disease

Intellectual developmental disorder, autosomal recessive 18, with or without epilepsy (MRT18):
A disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior and manifested during the developmental period.

Schiano, C., Luongo, L., Maione, S., & Napoli, C. (2023). Mediator complex in neurological disease. Life Sciences, 121986.

Prusty, A., Sharma, S., Malik, N., Kapoor, S., & Tyagi, A. K. (2023). Functional Versatility of Multi-Protein Mediator Complex in Plant Growth and Development. Critical Reviews in Plant Sciences, 1-39.

Chen, G. Y., Zhang, S., Li, C. H., Qi, C. C., Wang, Y. Z., Chen, J. Y., ... & Su, C. J. (2020). Mediator Med23 regulates adult hippocampal neurogenesis. Frontiers in Cell and Developmental Biology, 8, 699.

Zhang, S., O’Regan, R., & Xu, W. (2020). The emerging role of mediator complex subunit 12 in tumorigenesis and response to chemotherapeutics. Cancer, 126(5), 939-948.

Napoli, C., Schiano, C., & Soricelli, A. (2019). Increasing evidence of pathogenic role of the Mediator (MED) complex in the development of cardiovascular diseases. Biochimie, 165, 1-8.

Wang, Z., Cao, D., Li, C., Min, L., & Wang, G. (2019). Mediator MED23 regulates inflammatory responses and liver fibrosis. PLoS biology, 17(12), e3000563.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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