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Mouse Anti-MED25 (AA 1-146) Recombinant Antibody (CBFYM-2005) (CBMAB-M2183-FY)

This product is mouse antibody that recognizes MED25. The antibody CBFYM-2005 can be used for immunoassay techniques such as: ELISA, ICC, IF.
See all MED25 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYM-2005
Antibody Isotype
IgG2b, k
Application
ELISA, ICC, IF

Basic Information

Immunogen
Full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2b, k
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 1-146

Target

Full Name
MEDIATOR COMPLEX SUBUNIT 25
Introduction
This gene encodes a component of the transcriptional coactivator complex termed the Mediator complex. This complex is required for transcription of most RNA polymerase II-dependent genes. The encoded protein plays a role in chromatin modification and in preinitiation complex assembly. Mutations in this gene are associated with Charcot-Marie-Tooth disease type 2B2.
Entrez Gene ID
81857
UniProt ID
Q71SY5
Alternative Names
Mediator Complex Subunit 25; Activator Interaction Domain-Containing Protein 1; Activator-Recruited Cofactor 92 KDa Component; ACID1; ARC92; PTOV2; P78; Mediator Of RNA Polymerase II Transcription, Subunit 25 Homolog (S. Cerevisiae)
Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for RARA/RXRA-mediated transcription.
Biological Process
Negative regulation of fibroblast proliferation Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: UniProtKB
Positive regulation of chromatin binding Source: UniProtKB
Positive regulation of mediator complex assembly Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: UniProtKB
Positive regulation of transcription elongation from RNA polymerase II promoter Source: ComplexPortal
Positive regulation of transcription initiation from RNA polymerase II promoter Source: ComplexPortal
RNA polymerase II preinitiation complex assembly Source: ComplexPortal
Cellular Location
Nucleus
Involvement in disease
Charcot-Marie-Tooth disease 2B2 (CMT2B2):
A recessive axonal form of Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathies (designated CMT1 when they are dominantly inherited) and primary peripheral axonal neuropathies (CMT2). Neuropathies of the CMT2 group are characterized by signs of axonal degeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy.
Basel-Vanagaite-Smirin-Yosef syndrome (BVSYS):
An autosomal recessive syndrome characterized by eye, brain, cardiac and palatal abnormalities as well as growth retardation, microcephaly and severe intellectual disability.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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