Mouse Anti-MED8 Antibody (1B4) (CBMAB-0655-YC)

Name: Mouse Anti-MED8 Antibody (1B4)
SKU: CBMAB-0655-YC
Rating: 5 (1 reviews)

See all MED8 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1B4

Application

IP, WB, MA

Immunogen

Recombinant protein

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Supernatant

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

MED8

Introduction

MED8 (mediator of RNA polymerase II transcription subunit 8) is a protein component of the mediator complex, which aids in transcriptional activation through interaction with RNA polymerase II and gene-specific transcription factors. MED8 may also function in ubiquitin ligation and protein degradation.

Entrez Gene ID

112950

UniProt ID

Q96G25

Alternative Names

ARC32; MGC17544; MGC19641

Function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May play a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins.

Biological Process

Positive regulation of transcription elongation from RNA polymerase II promoter Source: ComplexPortal
Positive regulation of transcription initiation from RNA polymerase II promoter Source: ComplexPortal
Protein ubiquitination Source: UniProtKB-UniPathway
Regulation of transcription by RNA polymerase II Source: GO_Central
RNA polymerase II preinitiation complex assembly Source: ComplexPortal

Cellular Location

Nucleus

Wu, S., Li, Q., Cao, Y., Luo, S., Wang, Z., & Zhang, T. (2022). Mediator complex subunit 8 is a prognostic biomarker in hepatocellular carcinoma. American Journal of Translational Research, 14(3), 1765.

He, H., Denecker, J., Van Der Kelen, K., Willems, P., Pottie, R., Phua, S. Y., ... & Mhamdi, A. (2021). The Arabidopsis mediator complex subunit 8 regulates oxidative stress responses. The Plant Cell, 33(6), 2032-2057.

Wu, S., Cao, Y., Luo, S., Cao, S., Li, Q., & Zhang, T. (2021). High Expression of Mediator Complex Subunit 8 Acts as a Prognostic Biomarker in Hepatocellular Carcinoma.

Zhang, L., Song, Y., Liu, K., & Gong, F. (2021). The tomato Mediator subunit MED8 positively regulates plant response to Botrytis cinerea. Journal of Plant Physiology, 266, 153533.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-MED8 Antibody (1B4)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-MED8 Recombinant Antibody (1D3) (CAT#: CBMAB-A5380-LY)

Mouse Anti-MED8 Antibody (1A4) (CAT#: CBMAB-0654-YC)

Mouse Anti-MED8 (AA 1-59) Recombinant Antibody (CBFYM-2020) (CAT#: CBMAB-M2199-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry