Mouse Anti-MED9 (AA 1-146) Recombinant Antibody (CBFYM-2021) (CBMAB-M2200-FY)

Name: Mouse Anti-MED9 (AA 1-146) Recombinant Antibody (CBFYM-2021)
SKU: CBMAB-M2200-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

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Basic Information

Host Animal

Mouse

Clone

CBFYM-2021

Application

ELISA, IF, WB

Immunogen

Full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.Immunogen sequence: MASAGVAAGR QAEDVLPPTS DQPLPDTKPL PPPQPPPVPA PQPQQSPAPR PQSPARAREE ENYSFLPLVH NIIKCMDKDS PEVHQDLNAL KSKFQEMRKL ISTMPGIHLS PEQQQQQLQS LREQVRTKNE LLQKYKSLCM F

Specificity

Human

Antibody Isotype

IgG2b, k

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 1-146

More Infomation

Target

Full Name

MED9

Introduction

The multiprotein Mediator complex is a coactivator required for activation of RNA polymerase II transcription by DNA bound transcription factors. The protein encoded by this gene is thought to be a subunit of the Mediator complex. This gene is located within the Smith-Magenis syndrome region on chromosome 17.

Entrez Gene ID

55090

UniProt ID

Q9NWA0

Alternative Names

Mediator Complex Subunit 9; MED25; Mediator Of RNA Polymerase II Transcription, Subunit 9 Homolog (S. Cerevisiae); Mediator Of RNA Polymerase II Transcription, Subunit 9 Homolog; Mediator Of RNA Polymerase II Transcription Subunit 9; Mediator Subunit 25

Function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Biological Process

Positive regulation of transcription elongation from RNA polymerase II promoter Source: ComplexPortal
Positive regulation of transcription initiation from RNA polymerase II promoter Source: ComplexPortal
RNA polymerase II preinitiation complex assembly Source: ComplexPortal

Cellular Location

Nucleus

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Mouse Anti-MED9 Recombinant Antibody (CBFYM-0618) (CAT#: CBMAB-M0752-FY)

Mouse Anti-MED9 Recombinant Antibody (7F1) (CAT#: CBMAB-A5381-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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