Rat Anti-MEP1B (AA 23-293) Recombinant Antibody (CBFYM-2076) (CBMAB-M2257-FY)

Name: Rat Anti-MEP1B (AA 23-293) Recombinant Antibody (CBFYM-2076)
SKU: CBMAB-M2257-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

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Basic Information

Host Animal

Rat

Clone

CBFYM-2076

Application

WB, IHC

Immunogen

Human embryonic kidney cell line HEK293-derived recombinant human Meprin beta Subunit/MEP1B, Thr23-Ser293

Specificity

Human, Mouse

Antibody Isotype

IgG1

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS

Concentration

LYOPH

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 23-293

More Infomation

Target

Full Name

MEPRIN A SUBUNIT BETA

Introduction

Meprins are multidomain zinc metalloproteases that are highly expressed in mammalian kidney and intestinal brush border membranes, and in leukocytes and certain cancer cells. They are involved in the hydrolysis of a variety of peptide and protein substrates, and have been implicated in cancer and intestinal inflammation. Mature meprins are oligomers of evolutionarily related, but separately encoded alpha and/or beta subunits. Homooligomers of alpha subunit are secreted, whereas, oligomers containing the beta subunit are plasma membrane-bound. This gene encodes the beta subunit. Targeted disruption of this gene in mice affects embryonic viability, renal gene expression profiles, and distribution of the membrane-associated alpha subunit in kidney and intestine.

Entrez Gene ID

Human	4225
Mouse	17288

UniProt ID

Human	Q16820
Mouse	Q61847

Alternative Names

Meprin A Subunit Beta; N-Benzoyl-L-Tyrosyl-P-Amino-Benzoic Acid Hydrolase Subunit Beta; PABA Peptide Hydrolase; Endopeptidase-2; Meprin A, Beta; Meprin B; PPH Beta; N-Benzoyl-L-Tyrosyl-P-Amino-Benzoic Acid Hydrolase Beta Subunit; EC 3.4.24.63

Function

Membrane metallopeptidase that sheds many membrane-bound proteins. Exhibits a strong preference for acidic amino acids at the P1' position. Known substrates include: FGF19, VGFA, IL1B, IL18, procollagen I and III, E-cadherin, KLK7, gastrin, ADAM10, tenascin-C. The presence of several pro-inflammatory cytokine among substrates implicate MEP1B in inflammation. It is also involved in tissue remodeling due to its capability to degrade extracellular matrix components.

Biological Process

Inflammatory response Source: UniProtKB-KW
Toxin transport Source: Ensembl

Cellular Location

Secreted
Plasma membrane
Cell membrane
Note: Homodimers are essentially membrane bound but may also be shed from the surface by ADAM-10 and ADAM-17.

Topology

Extracellular: 23-652
Helical: 653-673
Cytoplasmic: 674-701

PTM

N-glycosylated; contains high mannose and/or complex biantennary structures.
O-glycosylation protect the C-terminal region from proteolytic cleavage and diminish secretion, this seems to be specific to human.
Proteolytically activated by trypsin in the intestinal lumen and kallikrein-related peptidases in other tissues.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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