Mouse Anti-METTL14 Recombinant Antibody (CBFYM-2122) (CBMAB-M2304-FY)

Name: Mouse Anti-METTL14 Recombinant Antibody (CBFYM-2122)
SKU: CBMAB-M2304-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYM-2122

Application

WB, IF, IHC-P

Immunogen

Recombinant protein corresponding to the following amino acid sequence:RSWNMDSRLQEIRERQKLRRQLLAQQLGAESADSIGAVLNSKDEQREIAETRETCRASYDTSAPNAKRKYLDEGETDEDKMEEYKDELEMQQDEE

Specificity

Human

Antibody Isotype

IgG2a

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Methyltransferase Like 14

Introduction

METTL14 is a Protein Coding gene. Among its related pathways are Gene Expression and mRNA Splicing - Major Pathway. Gene Ontology annotations related to this gene include RNA binding and mRNA -methyltransferase activity.

Entrez Gene ID

57721

UniProt ID

Q9HCE5

Alternative Names

Methyltransferase Like 14; Methyltransferase-Like Protein 14; HMETTL14; N6-Adenosine-Methyltransferase Non-Catalytic Subunit; N6-Adenosine-Methyltransferase Subunit METTL14; KIAA1627

Function

The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues at the N6 position of some mRNAs and regulates the circadian clock, differentiation of embryonic stem cells and cortical neurogenesis (PubMed:24316715, PubMed:24407421, PubMed:25719671, PubMed:29348140, PubMed:27373337, PubMed:27281194).

In the heterodimer formed with METTL3, METTL14 constitutes the RNA-binding scaffold that recognizes the substrate rather than the catalytic core (PubMed:27627798, PubMed:27373337, PubMed:27281194, PubMed:29348140).

N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in mRNA stability and processing (PubMed:24316715, PubMed:24407421, PubMed:25719671).

M6A acts as a key regulator of mRNA stability by promoting mRNA destabilization and degradation (By similarity).

In embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization (By similarity).

M6A regulates spermatogonial differentiation and meiosis and is essential for male fertility and spermatogenesis (By similarity).

M6A also regulates cortical neurogenesis: m6A methylation of transcripts related to transcription factors, neural stem cells, the cell cycle and neuronal differentiation during brain development promotes their destabilization and decay, promoting differentiation of radial glial cells (By similarity).

Biological Process

Forebrain radial glial cell differentiation Source: UniProtKB
Gliogenesis Source: UniProtKB
mRNA catabolic process Source: Ensembl
mRNA destabilization Source: UniProtKB
mRNA methylation Source: UniProtKB
mRNA splicing, via spliceosome Source: UniProtKB
Negative regulation of hematopoietic progenitor cell differentiation Source: ARUK-UCL
Positive regulation of translation Source: ARUK-UCL
Spermatogenesis Source: UniProtKB
Stem cell population maintenance Source: UniProtKB

Cellular Location

Nucleus

Xie, Q., Li, Z., Luo, X., Wang, D., Zhou, Y., Zhao, J., ... & Sun, T. (2022). piRNA-14633 promotes cervical cancer cell malignancy in a METTL14-dependent m6A RNA methylation manner. Journal of Translational Medicine, 20(1), 51.

Guan, Q., Lin, H., Miao, L., Guo, H., Chen, Y., Zhuo, Z., & He, J. (2022). Functions, mechanisms, and therapeutic implications of METTL14 in human cancer. Journal of hematology & oncology, 15(1), 1-15.

Liu, X., Du, Y., Huang, Z., Qin, H., Chen, J., & Zhao, Y. (2022). Insights into roles of METTL14 in tumors. Cell proliferation, 55(1), e13168.

Liu, X., Xiao, M., Zhang, L., Li, L., Zhu, G., Shen, E., ... & Sun, Z. (2021). The m6A methyltransferase METTL14 inhibits the proliferation, migration, and invasion of gastric cancer by regulating the PI3K/AKT/mTOR signaling pathway. Journal of clinical laboratory analysis, 35(3), e23655.

Zhou, H., Yin, K., Zhang, Y., Tian, J., & Wang, S. (2021). The RNA m6A writer METTL14 in cancers: Roles, structures, and applications. Biochimica et Biophysica Acta (BBA)-Reviews on Cancer, 1876(2), 188609.

Liu, P., Li, F., Lin, J., Fukumoto, T., Nacarelli, T., Hao, X., ... & Zhang, R. (2021). m6A-independent genome-wide METTL3 and METTL14 redistribution drives the senescence-associated secretory phenotype. Nature cell biology, 23(4), 355-365.

Liu, X., Qin, J., Gao, T., Li, C., Chen, X., Zeng, K., ... & Wang, S. (2020). Analysis of METTL3 and METTL14 in hepatocellular carcinoma. Aging (Albany NY), 12(21), 21638.

Yang, X., Zhang, S., He, C., Xue, P., Zhang, L., He, Z., ... & Zheng, M. (2020). METTL14 suppresses proliferation and metastasis of colorectal cancer by down-regulating oncogenic long non-coding RNA XIST. Molecular cancer, 19, 1-16.

Wang, M., Liu, J., Zhao, Y., He, R., Xu, X., Guo, X., ... & Qin, R. (2020). Upregulation of METTL14 mediates the elevation of PERP mRNA N6 adenosine methylation promoting the growth and metastasis of pancreatic cancer. Molecular cancer, 19(1), 1-15.

Huang, J., Dong, X., Gong, Z., Qin, L. Y., Yang, S., Zhu, Y. L., ... & Tang, C. (2019). Solution structure of the RNA recognition domain of METTL3-METTL14 N6-methyladenosine methyltransferase. Protein & cell, 10(4), 272-284.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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