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Mouse Anti-METTL16 Recombinant Antibody (CBFYM-2123) (CBMAB-M2305-FY)

This product is mouse antibody that recognizes METTL16. The antibody CBFYM-2123 can be used for immunoassay techniques such as: WB, IF, FC.
See all METTL16 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYM-2123
Antibody Isotype
IgG1
Application
WB, IF, FC

Basic Information

Immunogen
Full length human recombinant protein of human METT10D(NP_076991) produced in HEK293T cell
Specificity
Human
Antibody Isotype
IgG1
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
METHYLTRANSFERASE LIKE 16
Introduction
METTL16 is a Protein Coding gene. Gene Ontology annotations related to this gene include 23S rRNA -N)-methyltransferase activity.
Entrez Gene ID
79066
UniProt ID
Q86W50
Alternative Names
Methyltransferase Like 16; Methyltransferase 10 Domain-Containing Protein; Methyltransferase 10 Domain Containing; N6-Adenosine-Methyltransferase METTL16; Methyltransferase-Like Protein 16; U6 SnRNA Methyltransferase
Function
RNA N6-methyltransferase that methylates adenosine residues at the N6 position of a subset of RNAs and is involved in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts (PubMed:28525753, PubMed:30197299, PubMed:30197297, PubMed:33428944, PubMed:33930289).

Able to N6-methylate a subset of mRNAs and U6 small nuclear RNAs (U6 snRNAs) (PubMed:28525753).

In contrast to the METTL3-METTL14 heterodimer, only able to methylate a limited number of RNAs: requires both a 5'UACAGAGAA-3' nonamer sequence and a specific RNA structure (PubMed:28525753, PubMed:30197299, PubMed:30197297).

Plays a key role in S-adenosyl-L-methionine homeostasis by mediating N6-methylation of MAT2A mRNAs, altering splicing of MAT2A transcripts: in presence of S-adenosyl-L-methionine, binds the 3'-UTR region of MAT2A mRNA and specifically N6-methylates the first hairpin of MAT2A mRNA, preventing recognition of their 3'-splice site by U2AF1/U2AF35, thereby inhibiting splicing and protein production of S-adenosylmethionine synthase (PubMed:28525753, PubMed:33930289).

In S-adenosyl-L-methionine-limiting conditions, binds the 3'-UTR region of MAT2A mRNA but stalls due to the lack of a methyl donor, preventing N6-methylation and promoting expression of MAT2A (PubMed:28525753).

In addition to mRNAs, also able to mediate N6-methylation of U6 small nuclear RNA (U6 snRNA): specifically N6-methylates adenine in position 43 of U6 snRNAs (PubMed:28525753, PubMed:29051200, PubMed:32266935).

Also able to bind various lncRNAs, such as 7SK snRNA (7SK RNA) or 7SL RNA (PubMed:29051200).

Specifically binds the 3'-end of the MALAT1 long non-coding RNA (PubMed:27872311).
Biological Process
mRNA catabolic process Source: UniProtKB
mRNA destabilization Source: UniProtKB
mRNA methylation Source: UniProtKB
Negative regulation of 3'-UTR-mediated mRNA stabilization Source: UniProtKB
Posttranscriptional regulation of gene expression Source: UniProtKB
Regulation of mRNA splicing, via spliceosome Source: UniProtKB
rRNA base methylation Source: GO_Central
S-adenosylmethionine biosynthetic process Source: UniProtKB
snRNA (adenine-N6)-methylation Source: UniProtKB
Cellular Location
Cytoplasm
Nucleus

Mermoud, J. E. (2022). The Role of the m6A RNA Methyltransferase METTL16 in Gene Expression and SAM Homeostasis. Genes, 13(12), 2312.

Breger, K., & Brown, J. A. (2022). Elucidating the Kinetic Mechanism of Human METTL16. Biochemistry, 62(2), 494-506.

Dai, Y. Z., Liu, Y. D., Li, J., Chen, M. T., Huang, M., Wang, F., ... & Sun, S. H. (2022). METTL16 promotes hepatocellular carcinoma progression through downregulating RAB11B-AS1 in an m6A-dependent manner. Cellular & Molecular Biology Letters, 27(1), 41.

Satterwhite, E. R., & Mansfield, K. D. (2022). RNA methyltransferase METTL16: Targets and function. Wiley Interdisciplinary Reviews: RNA, 13(2), e1681.

Su, R., Dong, L., Li, Y., Gao, M., He, P. C., Liu, W., ... & Chen, J. (2022). METTL16 exerts an m6A-independent function to facilitate translation and tumorigenesis. Nature cell biology, 24(2), 205-216.

Zeng, X., Zhao, F., Cui, G., Zhang, Y., Deshpande, R. A., Chen, Y., ... & Lou, Z. (2022). METTL16 antagonizes MRE11-mediated DNA end resection and confers synthetic lethality to PARP inhibition in pancreatic ductal adenocarcinoma. Nature Cancer, 3(9), 1088-1104.

Ruszkowska, A. (2021). METTL16, methyltransferase-like protein 16: Current insights into structure and function. International Journal of Molecular Sciences, 22(4), 2176.

Wang, X. K., Zhang, Y. W., Wang, C. M., Li, B., Zhang, T. Z., Zhou, W. J., ... & He, Y. L. (2021). METTL16 promotes cell proliferation by up‐regulating cyclin D1 expression in gastric cancer. Journal of cellular and molecular medicine, 25(14), 6602-6617.

Nance, D. J., Satterwhite, E. R., Bhaskar, B., Misra, S., Carraway, K. R., & Mansfield, K. D. (2020). Characterization of METTL16 as a cytoplasmic RNA binding protein. PLoS One, 15(1), e0227647.

Aoyama, T., Yamashita, S., & Tomita, K. (2020). Mechanistic insights into m6A modification of U6 snRNA by human METTL16. Nucleic acids research, 48(9), 5157-5168.

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For research use only. Not intended for any clinical use.

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