Mouse Anti-MFHAS1 Recombinant Antibody (CBFYM-2153) (CBMAB-M2335-FY)

Name: Mouse Anti-MFHAS1 Recombinant Antibody (CBFYM-2153)
SKU: CBMAB-M2335-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYM-2153

Application

WB, IHC

Specificity

Rat

Antibody Isotype

IgG1

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Concentration

0.25 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

MALIGNANT FIBROUS HISTIOCYTOMA AMPLIFIED SEQUENCE 1

Introduction

Identified in a human 8p amplicon, this gene is a potential oncogene whose expression is enhanced in some malignant fibrous histiocytomas. The primary structure of its product includes an ATP/GTP-binding site, three leucine zipper domains, and a leucine-rich tandem repeat, which are structural or functional elements for interactions among proteins related to the cell cycle, and which suggest that overexpression might be oncogenic with respect to MFH.

Entrez Gene ID

306508

UniProt ID

D3ZJL1

Alternative Names

Malignant Fibrous Histiocytoma Amplified Sequence 1; Malignant Fibrous Histiocytoma-Amplified Sequences With Leucine-Rich Tandem Repeats 1; Malignant Fibrous Histiocytoma-Amplified Sequence With Leucine-Rich Tandem Repeats 1; Leucine Rich Repeat Containing 65; MASL1; MFH-Amplified Sequences With Leucine-Rich Tandem Repeats 1; Malignant Fibrous Histiocytoma-Amplified Sequence 1; LRRC65; ROCO4

Function

Probable GTP-binding protein (PubMed:24286120).

Functions in innate immunity and more specifically the inflammatory response as a regulator of the Toll-like receptor TLR2 and TLR4 signaling pathways (PubMed:26599367, PubMed:28471450, PubMed:28609714).

Negatively regulates the part of the TLR4 signaling pathway that leads to the activation of the transcription factor AP-1. By retaining the phosphatase complex PP2A into the cytoplasm, prevents the dephosphorylation of the AP-1 subunit JUN which is required for proper activation of the transcription factor (PubMed:28609714).

Both inhibits and activates the TLR2-dependent signaling pathway (PubMed:26599367).

Positively regulates the TLR2 signaling pathway to activate specifically the downstream p38 and JNK MAP kinases and promote the polarization of macrophages toward the pro-inflammatory M1 phenotype (PubMed:28471450).

It may also play a role in the regulation of inflammation induced by high glucose through the PKB/AKT signaling pathway (PubMed:29168081).

Also involved in erythrocyte differentiation through activation of the ERK1/ERK2 signaling pathway (PubMed:23327923).

Biological Process

Erythrocyte differentiation Source: UniProtKB
Inflammatory response Source: UniProtKB
Innate immune response Source: UniProtKB
Negative regulation of inflammatory response Source: UniProtKB
Negative regulation of protein dephosphorylation Source: UniProtKB
Negative regulation of protein localization to nucleus Source: UniProtKB
Negative regulation of toll-like receptor 2 signaling pathway Source: UniProtKB
Negative regulation of toll-like receptor 4 signaling pathway Source: UniProtKB
Positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
Positive regulation of JNK cascade Source: UniProtKB
Positive regulation of p38MAPK cascade Source: UniProtKB
Positive regulation of protein kinase B signaling Source: UniProtKB
Positive regulation of toll-like receptor 2 signaling pathway Source: UniProtKB
Regulation of macrophage activation Source: UniProtKB

Cellular Location

Cytoplasm

Involvement in disease

A chromosomal aberration involving MFHAS1 may be a cause of B-cell lymphoma. Translocation t(8;14)(p23.1;q21) with a cryptic exon named '14q21 element'. The resulting fusion protein named 'chimeric MASL1' is tumorigenic in nude mice.

PTM

Ubiquitinated. Ubiquitination by PJA2 does not lead MFHAS1 to proteasomal degradation but positively regulates its function in polarization of macrophages.

Jin, Y., Zhai, M., Cao, R., Yu, H., Wu, C., & Liu, Y. (2023). Silencing MFHAS1 Induces Pyroptosis via the JNK-activated NF-ΚB/Caspase1/GSDMD Signal Axis in Breast Cancer. Current Pharmaceutical Design, 29(42), 3408-3420.

Wang, H., & Hu, Y. (2020). CircRNA malignant fibrous histiocytoma amplified Sequence 1 (MFHAS1) reduced inflammatory responses in a Colitis Model via SIRT1/NF-κB. Food Science and Technology, 41, 504-513.

Kumkhaek, C., LaChance, C., Aerbajinai, W., Zhu, J., & Rodgers, G. P. (2019). Role of MFHAS1 in regulating hepcidin expression via the BMP/SMAD and MAPK/ERK1/2 signalling pathways. British journal of haematology, 186(4), e108.

Zhong, J., Guo, C., Hou, W., Shen, N., & Miao, C. (2019). Effects of MFHAS1 on cognitive impairment and dendritic pathology in the hippocampus of septic rats. Life sciences, 235, 116822.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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