Mouse Anti-MGST1 Recombinant Antibody (CBFYM-2191) (CBMAB-M2373-FY)

Name: Mouse Anti-MGST1 Recombinant Antibody (CBFYM-2191)
SKU: CBMAB-M2373-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYM-2191

Application

Immunogen

Recombinant full length GST

Specificity

Human

Antibody Isotype

IgG2b, k

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.2

Preservative

0.09% Sodium azide

Concentration

0.5 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

MICROSOMAL GLUTATHIONE S-TRANSFERASE 1

Introduction

The MAPEG family consists of six human proteins, two of which are involved in the production of leukotrienes and prostaglandin E, important mediators of inflammation. Other family members, demonstrating glutathione S-transferase and peroxidase activities, are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. This gene encodes a protein that catalyzes the conjugation of glutathione to electrophiles and the reduction of lipid hydroperoxides. This protein is localized to the endoplasmic reticulum and outer mitochondrial membrane where it is thought to protect these membranes from oxidative stress. Several transcript variants, some non-protein coding and some protein coding, have been found for this gene.

Entrez Gene ID

4257

UniProt ID

P10620

Alternative Names

Microsomal Glutathione S-Transferase 1; Microsomal GST-1; Microsomal GST-I; EC 2.5.1.18; GST12; MGST; Glutathione S-Transferase 12; MGST-I

Function

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has a wide substrate specificity.

Biological Process

Cellular response to lipid hydroperoxide Source: UniProtKB
Glutathione transport Source: UniProtKB

Cellular Location

Endoplasmic reticulum
Microsome
Endoplasmic reticulum membrane
Mitochondrion
Mitochondrion outer membrane

Topology

Lumenal: 3-9
Helical: 10-33
Cytoplasmic: 34-62
Helical: 63-96
Lumenal: 97-99
Helical: 100-123
Cytoplasmic: 124-128
Helical: 129-148
Lumenal: 149-155

PTM

Peroxynitrite induces nitration at Tyr-93 which activates the enzyme.

Sun, Y., Xie, J., Zhu, J., & Yuan, Y. (2024). Bioinformatics and Machine Learning Methods Identified MGST1 and QPCT as Novel Biomarkers for Severe Acute Pancreatitis. Molecular Biotechnology, 1-20.

Li, Y., Xu, X., Wang, X., Zhang, C., Hu, A., & Li, Y. (2023). MGST1 Expression Is Associated with Poor Prognosis, Enhancing the Wnt/β-Catenin Pathway via Regulating AKT and Inhibiting Ferroptosis in Gastric Cancer. ACS Omega.

Cebula, M., & Morgenstern, R. (2023). Enzymology of reactive intermediate protection: kinetic analysis and temperature dependence of the mesophilic membrane protein catalyst MGST1. The FEBS Journal.

Yan, J., Ye, G., & Shao, Y. (2022). High expression of the ferroptosis‐associated MGST1 gene in relation to poor outcome and maladjusted immune cell infiltration in uterine corpus endometrial carcinoma. Journal of clinical laboratory analysis, 36(4), e24317.

Dai, H., & Lu, X. (2022). MGST1 alleviates the oxidative stress of trophoblast cells induced by hypoxia/reoxygenation and promotes cell proliferation, migration, and invasion by activating the PI3K/AKT/mTOR pathway. Open Medicine, 17(1), 2062-2071.

Kuang, F., Liu, J., Xie, Y., Tang, D., & Kang, R. (2021). MGST1 is a redox-sensitive repressor of ferroptosis in pancreatic cancer cells. Cell Chemical Biology, 28(6), 765-775.

Yang, B., Xia, S., Ye, X., Jing, W., & Wu, B. (2021). MiR-379–5p targets microsomal glutathione transferase 1 (MGST1) to regulate human glioma in cell proliferation, migration and invasion and epithelial-mesenchymal transition (EMT). Biochemical and biophysical research communications, 568, 8-14.

Dodson, M., Anandhan, A., & Zhang, D. D. (2021). MGST1, a new soldier of NRF2 in the battle against ferroptotic death. Cell Chemical Biology, 28(6), 741-742.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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