Mouse Anti-MIA Recombinant Antibody (3A6) (CBMAB-A5476-LY)

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Summary

Host Animal

Mouse

Specificity

Human

Clone

3A6

Antibody Isotype

IgG2a, κ

Application

sELISA, ELISA

Basic Information

Immunogen

MIA (AAH05910, 1 a.a. ~ 131 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

melanoma inhibitory activity

Entrez Gene ID

8190

UniProt ID

Q16674

Alternative Names

CD-RAP

Function

Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.

Biological Process

Extracellular matrix organization Source: GO_Central

Cellular Location

Secreted

PTM

May possess two intramolecular disulfide bonds.

More Infomation

References

Mehrabipour, M., Jasemi, N. S. K., Dvorsky, R., & Ahmadian, M. R. (2023). A Systematic Compilation of Human SH3 Domains: A Versatile Superfamily in Cellular Signaling. Cells, 12(16), 2054.

Arnolds, O., & Stoll, R. (2023). Characterization of a fold in TANGO1 evolved from SH3 domains for the export of bulky cargos. Nature Communications, 14(1), 2273.

Lei, Y., Xu, J., Li, M., Meng, T., Chen, M., Yang, Y., ... & Zuo, J. (2021). MIA SH3 Domain ER Export Factor 3 Deficiency Prevents Neointimal Formation by Restoring BAT-Like PVAT and Decreasing VSMC Proliferation and Migration. Frontiers in Endocrinology, 12, 748216.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

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Mouse Anti-MIA (AA 1-131) Recombinant Antibody (CBFYM-2196)

Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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