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Mouse Anti-MIF Recombinant Antibody (CBFYM-0226) (CBMAB-M0270-FY)

This product is mouse antibody that recognizes MIF. The antibody CBFYM-0226 can be used for immunoassay techniques such as: FC.
See all MIF antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYM-0226
Antibody Isotype
IgG1
Application
FC

Basic Information

Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Macrophage Migration Inhibitory Factor
Introduction
This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways.
Entrez Gene ID
4282
UniProt ID
P14174
Alternative Names
Macrophage Migration Inhibitory Factor; Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor); Glycosylation-Inhibiting Factor; Phenylpyruvate Tautomerase; L-Dopachrome Tautomerase; L-Dopachrome Isomerase
Function
Pro-inflammatory cytokine involved in the innate immune response to bacterial pathogens (PubMed:15908412, PubMed:17443469, PubMed:23776208).

The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense (PubMed:15908412, PubMed:17443469, PubMed:23776208).

Counteracts the anti-inflammatory activity of glucocorticoids (PubMed:15908412, PubMed:17443469, PubMed:23776208).

Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known (PubMed:11439086, PubMed:17526494).

It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity (PubMed:11439086, PubMed:17526494).
Biological Process
Carboxylic acid metabolic process Source: BHF-UCL
Cell aging Source: Ensembl
Cell surface receptor signaling pathway Source: UniProtKB
DNA damage response, signal transduction by p53 class mediator Source: Ensembl
Inflammatory response Source: UniProtKB-KW
Innate immune response Source: UniProtKB-KW
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of cell aging Source: BHF-UCL
Negative regulation of cell migration Source: ARUK-UCL
Negative regulation of cellular protein metabolic process Source: Ensembl
Negative regulation of DNA damage response, signal transduction by p53 class mediator Source: BHF-UCL
Negative regulation of gene expression Source: BHF-UCL
Negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator Source: BHF-UCL
Negative regulation of macrophage chemotaxis Source: BHF-UCL
Negative regulation of mature B cell apoptotic process Source: Ensembl
Negative regulation of myeloid cell apoptotic process Source: Ensembl
Positive regulation of arachidonic acid secretion Source: Ensembl
Positive regulation of B cell proliferation Source: BHF-UCL
Positive regulation of cell population proliferation Source: BHF-UCL
Positive regulation of chemokine (C-X-C motif) ligand 2 production Source: Ensembl
Positive regulation of cytokine production Source: BHF-UCL
Positive regulation of ERK1 and ERK2 cascade Source: BHF-UCL
Positive regulation of fibroblast proliferation Source: BHF-UCL
Positive regulation of lipopolysaccharide-mediated signaling pathway Source: Ensembl
Positive regulation of MAP kinase activity Source: Ensembl
Positive regulation of myeloid leukocyte cytokine production involved in immune response Source: Ensembl
Positive regulation of peptidyl-serine phosphorylation Source: BHF-UCL
positive regulation of peptidyl-tyrosine phosphorylation Source: BHF-UCL
Positive regulation of phosphorylation Source: BHF-UCL
Positive regulation of prostaglandin secretion involved in immune response Source: Ensembl
Positive regulation of protein kinase A signaling Source: BHF-UCL
Positive regulation of tumor necrosis factor production Source: BHF-UCL
Prostaglandin biosynthetic process Source: UniProtKB
Protein homotrimerization Source: UniProtKB
Regulation of macrophage activation Source: UniProtKB
Cellular Location
Cytoplasm
Secreted
Note: Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens.
Involvement in disease
Rheumatoid arthritis systemic juvenile (RASJ):
An inflammatory articular disorder with systemic onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.

Sumaiya, K., Langford, D., Natarajaseenivasan, K., & Shanmughapriya, S. (2022). Macrophage migration inhibitory factor (MIF): a multifaceted cytokine regulated by genetic and physiological strategies. Pharmacology & Therapeutics, 233, 108024.

Kong, Y. Z., Chen, Q., & Lan, H. Y. (2022). Macrophage migration inhibitory factor (MIF) as a stress molecule in renal inflammation. International Journal of Molecular Sciences, 23(9), 4908.

Osipyan, A., Chen, D., & Dekker, F. J. (2021). Epigenetic regulation in macrophage migration inhibitory factor (MIF)-mediated signaling in cancer and inflammation. Drug Discovery Today, 26(7), 1728-1734.

Koh, H. M., & Kim, D. C. (2020). Prognostic significance of macrophage migration inhibitory factor expression in cancer patients: A systematic review and meta-analysis. Medicine, 99(32).

Tilstam, P. V., Pantouris, G., Corman, M., Andreoli, M., Mahboubi, K., Davis, G., ... & Bucala, R. (2019). A selective small-molecule inhibitor of macrophage migration inhibitory factor-2 (MIF-2), a MIF cytokine superfamily member, inhibits MIF-2 biological activity. Journal of Biological Chemistry, 294(49), 18522-18531.

Sinitski, D., Kontos, C., Krammer, C., Asare, Y., Kapurniotu, A., & Bernhagen, J. (2019). Macrophage migration inhibitory factor (MIF)-based therapeutic concepts in atherosclerosis and inflammation. Thrombosis and Haemostasis, 119(04), 553-566.

Bilsborrow, J. B., Doherty, E., Tilstam, P. V., & Bucala, R. (2019). Macrophage migration inhibitory factor (MIF) as a therapeutic target for rheumatoid arthritis and systemic lupus erythematosus. Expert opinion on therapeutic targets, 23(9), 733-744.

Soumoy, L., Kindt, N., Ghanem, G., Saussez, S., & Journe, F. (2019). Role of macrophage migration inhibitory factor (MIF) in melanoma. Cancers, 11(4), 529.

de Souza, G. F., Muraro, S. P., Santos, L. D., Monteiro, A. P. T., da Silva, A. G., de Souza, A. P. D., ... & Porto, B. N. (2019). Macrophage migration inhibitory factor (MIF) controls cytokine release during respiratory syncytial virus infection in macrophages. Inflammation Research, 68, 481-491.

Guda, M. R., Rashid, M. A., Asuthkar, S., Jalasutram, A., Caniglia, J. L., Tsung, A. J., & Velpula, K. K. (2019). Pleiotropic role of macrophage migration inhibitory factor in cancer. American Journal of Cancer Research, 9(12), 2760.

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For research use only. Not intended for any clinical use.

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