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Mouse Anti-MKRN1 Recombinant Antibody (CBFYM-0651) (CBMAB-M0787-FY)

This product is mouse antibody that recognizes MKRN1. The antibody CBFYM-0651 can be used for immunoassay techniques such as: ELISA, WB.
See all MKRN1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYM-0651
Antibody Isotype
IgG2b, k
Application
ELISA, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG2b, k
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
makorin ring finger protein 1
Introduction
This gene encodes a protein that belongs to a novel class of zinc finger proteins. The encoded protein functions as a transcriptional co-regulator, and as an E3 ubiquitin ligase that promotes the ubiquitination and proteasomal degradation of target proteins. The protein encoded by this gene is thought to regulate RNA polymerase II-catalyzed transcription. Substrates for this protein's E3 ubiquitin ligase activity include the capsid protein of the West Nile virus and the catalytic subunit of the telomerase ribonucleoprotein. This protein controls cell cycle arrest and apoptosis by regulating p21, a cell cycle regulator, and the tumor suppressor protein p53. Pseudogenes of this gene are present on chromosomes 1, 3, 9, 12 and 20, and on the X chromosome. Alternative splicing results in multiple transcript variants encoding different isoforms.
Entrez Gene ID
23608
UniProt ID
Q9UHC7
Alternative Names
Makorin Ring Finger Protein 1; RING-Type E3 Ubiquitin Transferase Makorin-1; RING Finger Protein 61; RNF61; E3 Ubiquitin-Protein Ligase Makorin-1; EC 2.3.2.27
Function
E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include FILIP1, p53/TP53, CDKN1A and TERT. Keeps cells alive by suppressing p53/TP53 under normal conditions, but stimulates apoptosis by repressing CDKN1A under stress conditions. Acts as a negative regulator of telomerase. Has negative and positive effects on RNA polymerase II-dependent transcription.
Biological Process
Protein polyubiquitination Source: FlyBase
Protein ubiquitination Source: GO_Central
Cellular Location
Cytosol
PTM
Auto-ubiquitinated; which leads to proteasomal degradation.

Yang, Y., Luo, Y., Huang, S., Tao, Y., Li, C., & Wang, C. (2023). MKRN1/2 serve as tumor suppressors in renal clear cell carcinoma by regulating the expression of p53. Cancer Biomarkers, (Preprint), 1-12.

Zhang, Y., Li, Q. S., Liu, H. L., Tang, H. T., Yang, H. L., Wu, D. Q., ... & Li, M. X. (2023). MKRN1 promotes colorectal cancer metastasis by activating the TGF-β signalling pathway through SNIP1 protein degradation. Journal of Experimental & Clinical Cancer Research, 42(1), 219.

Kian, W., Krayim, B., Alsana, H., Giles, B., Purim, O., Alguayn, W., ... & Roisman, L. C. (2023). Overcoming CEP85L-ROS1, MKRN1-BRAF and MET amplification as rare, acquired resistance mutations to Osimertinib. Frontiers in Oncology, 13, 1124949.

Dou, Y., Xie, Y., Zhang, L., Liu, S., Xu, D., Wei, Y., ... & Zhang, X. L. (2022). Host MKRN1-mediated mycobacterial PPE protein ubiquitination suppresses innate immune response. Frontiers in Immunology, 13, 880315.

Cheng, C., Zhang, W., Zhang, C., Ji, P., Wu, X., Sha, Z., ... & Shi, L. (2021). Hyperoside ameliorates DSS-induced colitis through MKRN1-mediated regulation of PPARγ signaling and Th17/Treg balance. Journal of agricultural and food chemistry, 69(50), 15240-15251.

Bai, X., Yang, H., Pu, J., Zhao, Y., Jin, Y., & Yu, Q. (2021). MKRN1 ubiquitylates p21 to protect against intermittent hypoxia-induced myocardial apoptosis. Oxidative Medicine and Cellular Longevity, 2021.

Fang, Y. C., Fu, S. J., Hsu, P. H., Chang, P. T., Huang, J. J., Chiu, Y. C., ... & Jeng, C. J. (2021). Identification of MKRN1 as a second E3 ligase for Eag1 potassium channels reveals regulation via differential degradation. Journal of Biological Chemistry, 296.

Subrahmanian, M., Marimuthu, J., Sairam, T., & Sankaran, R. (2020). In vitro ubiquitination of Mycobacterium tuberculosis by E3 ubiquitin ligase, MKRN1. Biotechnology Letters, 42, 1527-1534.

Hildebrandt, A., Brüggemann, M., Rücklé, C., Boerner, S., Heidelberger, J. B., Busch, A., ... & Beli, P. (2019). The RNA-binding ubiquitin ligase MKRN1 functions in ribosome-associated quality control of poly (A) translation. Genome biology, 20, 1-20.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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