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Mouse Anti-MLF2 Recombinant Antibody (2F6-1E3) (CBMAB-A5509-LY)

The product is antibody recognizes MLF2. The antibody 2F6-1E3 immunoassay techniques such as: WB, ELISA.
See all MLF2 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

2F6-1E3

Antibody Isotype

IgG1, κ

Application

WB, ELISA

Basic Information

Immunogen

MLF2 (AAH00898, 1 a.a. ~ 248 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Myeloid Leukemia Factor 2

Entrez Gene ID

UniProt ID

Alternative Names

NTN4

Biological Process

Regulation of transcription, DNA-templated Source: GO_Central

Cellular Location

Nucleus
Cytoplasm

References

Fang, D., Hu, H., Zhao, K., Xu, A., Yu, C., Zhu, Y., ... & Mei, Y. (2023). MLF2 Negatively Regulates P53 and Promotes Colorectal Carcinogenesis. Advanced Science, 10(26), 2303336.

Ogunleye, A. J., Romanova, E., & Medvedeva, Y. A. (2021). Genome-wide regulation of CpG methylation by ecCEBPα in acute myeloid leukemia. F1000Research, 10.

Yang, J., Cao, D., Zhang, Y., Ou, R., Yin, Z., Liu, Y., ... & Fei, J. (2020). The role of phosphorylation of MLF2 at serine 24 in BCR-ABL leukemogenesis. Cancer Gene Therapy, 27(1-2), 98-107.

Huang, D., Liu, C., Sun, X., Sun, X., Qu, Y., Tang, Y., ... & Tong, T. (2020). CRL4DCAF8 and USP11 oppositely regulate the stability of myeloid leukemia factors (MLFs). Biochemical and Biophysical Research Communications, 529(2), 127-132.

Rampello, A. J., Laudermilch, E., Vishnoi, N., Prophet, S. M., Shao, L., Zhao, C., ... & Schlieker, C. (2020). Torsin ATPase deficiency leads to defects in nuclear pore biogenesis and sequestration of MLF2. Journal of Cell Biology, 219(6).

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Antibody Pairs

MLF2 Matched Antibody Pair (738)

MLF2 Matched Antibody Pair (739)

Related Products

MLF2 Matched Antibody Pair (738)

MLF2 Matched Antibody Pair (739)

Mouse Anti-MLF2 Recombinant Antibody (CBFYM-2284)

Mouse Anti-MLF2 (AA 1-248) Recombinant Antibody (CBFYM-2285)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Documents

Datasheet
SDS
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Second antibody and isotype control

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