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Mouse Anti-MLKL (Phospho-Ser358) Recombinant Antibody (EC210) (V2LY-0624-LY210)

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Datasheet
SDS
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Basic Information

Host Animal
Mouse
Clone
EC210
Application
IHC-P
Immunogen
Synthetic Peptide of Phospho-MLKL (Ser358).
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
IHC-P1:100-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4, 0.5% protective protein, 50% glycerol,
Preservative
0.02% sodium azide
Concentration
Batch dependent
Purity
>95% by SDS Page
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
Mixed Lineage Kinase Domain Like Pseudokinase
Introduction
This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene.
Entrez Gene ID
197259
UniProt ID
Q8NB16
Alternative Names
Mixed Lineage Kinase Domain Like Pseudokinase; HMLKL; Mixed Lineage Kinase Domain-Like Protein; Mixed Lineage Kinase Domain-Like;
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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