Mouse Anti-MRM3 Recombinant Antibody (A-5) (CBMAB-R0587-CN)

This product is a Mouse antibody that recognizes MRM3. The antibody A-5 can be used for immunoassay techniques such as: WB, IP, IF, ELISA.
See all MRM3 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Mouse, Rat, Human

Clone

A-5

Application

WB, IP, IF, ELISA

Basic Information

Specificity

Mouse, Rat, Human

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Target

Full Name

Mitochondrial RRNA Methyltransferase 3

Introduction

Efficient translation of mitochondrial-derived transcripts requires proper assembly of the large subunit of the mitochondrial ribosome. Central to the biogenesis of this large subunit is the A-loop of mitochondrial 16S rRNA, which is modified by three rRNA methyltransferases located near mtDNA nucleoids. The protein encoded by this gene methylates G(1370) of 16S rRNA, and this modification is necessary for proper ribosomal large subnit assembly. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2015]

Entrez Gene ID

Human	55178
Mouse	67390
Rat	360569

UniProt ID

Human	Q9HC36
Mouse	Q5ND52
Rat	D3ZQG0

Alternative Names

Mitochondrial RRNA Methyltransferase 3; 16S RRNA (Guanosine(1370)-2-O)-Methyltransferase; 16S RRNA [Gm1370] 2-O-Methyltransferase; RNA Methyltransferase-Like Protein 1; RNA Methyltransferase Like 1; RNMTL1;

Function

S-adenosyl-L-methionine-dependent 2'-O-ribose methyltransferase that catalyzes the formation of 2'-O-methylguanosine at position 1370 (Gm1370) in the 16S mitochondrial large subunit ribosomal RNA (mtLSU rRNA), a conserved modification in the peptidyl transferase domain of the mtLSU rRNA.

Biological Process

rRNA 2'-O-methylation Source: Reactome

Cellular Location

Mitochondrion

References

Rebelo-Guiomar, P., Pellegrino, S., Dent, K. C., Sas-Chen, A., Miller-Fleming, L., Garone, C., ... & Minczuk, M. (2022). A late-stage assembly checkpoint of the human mitochondrial ribosome large subunit. Nature communications, 13(1), 929.

Wang, P., Li, J., Wu, M., Ye, M., Huang, K., & Zhu, X. (2021). Human Mitochondrial Ribosomal RNA Modification-Based Classification Contributes to Discriminate the Prognosis and Immunotherapy Response of Glioma Patients. Frontiers in Immunology, 12, 722479.

Laptev, I., Dontsova, O., & Sergiev, P. (2020). Epitranscriptomics of mammalian mitochondrial ribosomal RNA. Cells, 9(10), 2181.

Laptev, I., Shvetsova, E., Levitskii, S., Serebryakova, M., Rubtsova, M., Bogdanov, A., ... & Dontsova, O. (2020). Mouse Trmt2B protein is a dual specific mitochondrial metyltransferase responsible for m5U formation in both tRNA and rRNA. RNA biology, 17(4), 441-450.

Haute, L. V., Hendrick, A. G., D’Souza, A. R., Powell, C. A., Rebelo-Guiomar, P., Harbour, M. E., ... & Minczuk, M. (2019). METTL15 introduces N4-methylcytidine into human mitochondrial 12S rRNA and is required for mitoribosome biogenesis. Nucleic acids research, 47(19), 10267-10281.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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