Mouse Anti-MRPS34 Recombinant Antibody (CBFYM-2584) (CBMAB-M2772-FY)

This product is mouse antibody that recognizes MRPS34. The antibody CBFYM-2584 can be used for immunoassay techniques such as: WB, IHC, IF.
See all MRPS34 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Dog

Clone

CBFYM-2584

Antibody Isotype

IgG1

Application

WB, IHC, IF

Basic Information

Immunogen

Full length human recombinant protein of human MRPS34(NP_076425) produced in E.coli

Specificity

Human, Dog

Antibody Isotype

IgG1

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.3, 1% BSA

Preservative

0.02% Sodium azide

Concentration

1 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

MITOCHONDRIAL RIBOSOMAL PROTEIN S34

Introduction

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. Alternative splicing results in multiple transcript variants.

Entrez Gene ID

Human	65993
Dog	100856578

UniProt ID

Human	P82930
Dog	E2R798

Alternative Names

Mitochondrial Ribosomal Protein S34; Mitochondrial Small Ribosomal Subunit Protein MS34; MRP-S34; S34mt; 28S Ribosomal Protein S34, Mitochondrial; Mitochondrial 28S Ribosomal Protein S34; MRP-S12; COXPD32; MRPS12

Function

Required for mitochondrial translation, plays a role in maintaining the stability of the small ribosomal subunit and the 12S rRNA that are required for mitoribosome formation.

Biological Process

Mitochondrial translation Source: ComplexPortal

Cellular Location

Mitochondrion

Involvement in disease

Combined oxidative phosphorylation deficiency 32 (COXPD32):
An autosomal recessive disorder due to deficiency of mitochondrial respiratory chain complexes, I, III and IV, and characterized by delayed psychomotor development and neurodevelopmental regression. Additional variable symptoms include poor or absent speech, inability to walk, and abnormal movements.

References

Soares, M. P., Travessa, A. M., Custódio, S., Pereira, C., Pinto, P., & Sousa, A. B. (2024). Uniparental Disomy as a Mechanism for Combined Oxidative Phosphorylation Deficiency Associated with MRPS34 Gene. Endocrine, Metabolic & Immune Disorders Drug Targets.

Shen, M. X., Ji, X. N., Wu, F., Gao, Y. Y., Feng, S., Xie, L. N., ... & Chen, Q. (2023). A case of combined oxidative phosphorylation deficiency 32 caused by MRPS34 gene variation and literature review. Zhonghua er ke za zhi= Chinese Journal of Pediatrics, 61(7), 642-647.

Lenzini, L., Carecchio, M., Iori, E., Legati, A., Lamantea, E., Avogaro, A., & Vitturi, N. (2022). A novel MRPS34 gene mutation with combined OXPHOS deficiency in an adult patient with Leigh syndrome. Molecular Genetics and Metabolism Reports, 30, 100830.

Lake, N. J., Webb, B. D., Stroud, D. A., Richman, T. R., Ruzzenente, B., Compton, A. G., ... & Thorburn, D. R. (2019). Biallelic mutations in MRPS34 lead to instability of the small mitoribosomal subunit and Leigh syndrome. European Journal of Human Genetics, 27, 860-861.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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