Mouse Anti-MSH5 Recombinant Antibody (1C11) (CBMAB-A5607-LY)

Name: Mouse Anti-MSH5 Recombinant Antibody (1C11)
SKU: CBMAB-A5607-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1C11

Application

WB, ELISA

Immunogen

MSH5 (AAH02498, 736 a.a. ~ 835 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

mutS homolog 5 (E. coli)

Introduction

This gene encodes a member of the mutS family of proteins that are involved in DNA mismatch repair or meiotic recombination processes. This protein is similar to a Saccharomyces cerevisiae protein that participates in meiotic segregation fidelity and crossing-over. This protein forms heterooligomers with another member of this family, mutS homolog 4. Alternative splicing results in four transcript variants encoding three different isoforms. [provided by RefSeq]

Entrez Gene ID

4439

UniProt ID

O43196

Alternative Names

DKFZp434C1615; G7; MGC2939; MutSH5; NG23

Function

Involved in DNA mismatch repair and meiotic recombination processes. Facilitates crossovers between homologs during meiosis (By similarity).

Biological Process

Chiasma assembly Source: GO_Central
Mismatch repair Source: InterPro

Cellular Location

Nucleus

Involvement in disease

Premature ovarian failure 13 (POF13):
An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.

Wyrwoll, M. J., Van Walree, E. S., Hamer, G., Rotte, N., Motazacker, M. M., Meijers-Heijboer, H., ... & Tüttelmann, F. (2022). Bi-allelic variants in DNA mismatch repair proteins MutS Homolog MSH4 and MSH5 cause infertility in both sexes. Human Reproduction, 37(1), 178-189.

Gong, C., Abbas, T., Muhammad, Z., Zhou, J., Khan, R., Ma, H., ... & Shi, B. (2022). A homozygous loss-of-function mutation in MSH5 abolishes MutSγ axial loading and causes meiotic arrest in NOA-affected individuals. International Journal of Molecular Sciences, 23(12), 6522.

Chen, M., Yao, C., Qin, Y., Cui, X., Li, P., Ji, Z., ... & Gao, F. (2022). Mutations of MSH5 in nonobstructive azoospermia (NOA) and rescued via in vivo gene editing. Signal Transduction and Targeted Therapy, 7(1), 1.

Desjardins, S. D., Ogle, D. E., Ayoub, M. A., Heckmann, S., Henderson, I. R., Edwards, K. J., & Higgins, J. D. (2020). MutS homologue 4 and MutS homologue 5 maintain the obligate crossover in wheat despite stepwise gene loss following polyploidization. Plant Physiology, 183(4), 1545-1558.

AbdelGawwad, M. R., Marić, A., Al-Ghamdi, A. A., & Hatamleh, A. A. (2019). Interactome analysis and docking sites of MutS homologs reveal new physiological roles in Arabidopsis thaliana. Molecules, 24(13), 2493.

Milano, C. R., Holloway, J. K., Zhang, Y., Jin, B., Smith, C., Bergman, A., ... & Cohen, P. E. (2019). Mutation of the ATPase domain of MutS homolog-5 (MSH5) reveals a requirement for a functional MutSγ complex for all crossovers in mammalian meiosis. G3: Genes, Genomes, Genetics, 9(6), 1839-1850.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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