Mouse Anti-MYOG Recombinant Antibody (CBFYM-0772) (CBMAB-M0915-FY)

Name: Mouse Anti-MYOG Recombinant Antibody (CBFYM-0772)
SKU: CBMAB-M0915-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYM-0772

Application

IF, IHC, IP, WB

Specificity

Rat

Antibody Isotype

IgG1, k

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.2

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Myogenin

Introduction

Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture. It is a member of a large family of proteins related by sequence homology, the helix-loop-helix proteins. It is essential for the development of functional skeletal muscle.

Entrez Gene ID

29148

UniProt ID

P20428

Alternative Names

Myogenin; Myogenic Factor 4; Class C Basic Helix-Loop-Helix Protein 3; BHLHc3; Myf-4; MYF4; Myogenin (Myogenic Factor 4)

Function

Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation, cell cycle exit and muscle atrophy. Essential for the development of functional embryonic skeletal fiber muscle differentiation. However is dispensable for postnatal skeletal muscle growth; phosphorylation by CAMK2G inhibits its transcriptional activity in respons to muscle activity. Required for the recruitment of the FACT complex to muscle-specific promoter regions, thus promoting gene expression initiation. During terminal myoblast differentiation, plays a role as a strong activator of transcription at loci with an open chromatin structure previously initiated by MYOD1. Together with MYF5 and MYOD1, co-occupies muscle-specific gene promoter core regions during myogenesis. Cooperates also with myocyte-specific enhancer factor MEF2D and BRG1-dependent recruitment of SWI/SNF chromatin-remodeling enzymes to alter chromatin structure at myogenic late gene promoters. Facilitates cell cycle exit during terminal muscle differentiation through the up-regulation of miR-20a expression, which in turn represses genes involved in cell cycle progression. Binds to the E-box containing (E1) promoter region of the miR-20a gene. Plays also a role in preventing reversal of muscle cell differentiation. Contributes to the atrophy-related gene expression in adult denervated muscles. Induces fibroblasts to differentiate into myoblasts (By similarity).

Biological Process

Cell cycle Source: UniProtKB-KW
Cellular response to estradiol stimulus Source: UniProtKB
Cellular response to growth factor stimulus Source: Ensembl
Cellular response to lithium ion Source: Ensembl
Cellular response to tumor necrosis factor Source: Ensembl
Muscle cell fate commitment Source: BHF-UCL
Negative regulation of cell population proliferation Source: UniProtKB
Ossification Source: Ensembl
Positive regulation of muscle atrophy Source: UniProtKB
Positive regulation of myoblast differentiation Source: UniProtKB
Positive regulation of myotube differentiation Source: UniProtKB
Positive regulation of skeletal muscle fiber development Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: NTNU_SB
Regulation of cell cycle Source: UniProtKB
Regulation of myoblast fusion Source: UniProtKB
Regulation of skeletal muscle satellite cell proliferation Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: GO_Central
Response to denervation involved in regulation of muscle adaptation Source: UniProtKB
Response to electrical stimulus involved in regulation of muscle adaptation Source: UniProtKB
Response to muscle activity involved in regulation of muscle adaptation Source: UniProtKB
Skeletal muscle cell differentiation Source: GO_Central
Skeletal muscle fiber development Source: Ensembl
Skeletal muscle tissue development Source: ProtInc
Striated muscle atrophy Source: UniProtKB

Cellular Location

Nucleus
Note: Recruited to late myogenic gene promoter regulatory sequences with SMARCA4/BRG1/BAF190A and SWI/SNF chromatin-remodeling enzymes to promote chromatin-remodeling and transcription initiation in developing embryos.

PTM

Phosphorylated by CAMK2G on threonine and serine amino acids in a muscle activity-dependent manner. Phosphorylation of Thr-87 impairs both DNA-binding and trans-activation functions in contracting muscles (By similarity).

Yin, Y., Chen, G., Lin, Z., Zhang, D., Lin, W., & Luo, W. (2023). Natural antisense transcript of MYOG regulates development and regeneration in skeletal muscle by shielding the binding sites of MicroRNAs of MYOG mRNA 3′ UTR. Biochemical and Biophysical Research Communications, 662, 93-103.

Gellhaus, B., Böker, K. O., Gsaenger, M., Rodenwaldt, E., Hüser, M. A., Schilling, A. F., & Saul, D. (2023). Foxo3 knockdown mediates decline of Myod1 and Myog reducing myoblast conversion to myotubes. Cells, 12(17), 2167.

Vicente-Garcia, C., Hernández-Camacho, J. D., & Carvajal, J. J. (2022). Regulation of myogenic gene expression. Experimental Cell Research, 113299.

Wei, D., Zhang, J., Raza, S. H. A., Song, Y., Jiang, C., Song, X., ... & Quan, G. (2022). Interaction of MyoD and MyoG with Myoz2 gene in bovine myoblast differentiation. Research in Veterinary Science, 152, 569-578.

Li, P., Zhang, X., Tian, L., Zhao, Y., Yan, Y., Li, S., ... & Tong, H. (2022). Vitamin C Promotes Muscle Development Mediated by the Interaction of CSRP3 with MyoD and MyoG. Journal of Agricultural and Food Chemistry, 70(23), 7158-7169.

Shen, X., Cui, C., Tang, S., Han, S., Zhang, Y., Xia, L., ... & Yin, H. (2022). MyoG-enhanced circGPD2 regulates chicken skeletal muscle development by targeting miR-203a. International Journal of Biological Macromolecules, 222, 2212-2224.

Adhikari, A., Kim, W., & Davie, J. (2021). Myogenin is required for assembly of the transcription machinery on muscle genes during skeletal muscle differentiation. PLoS One, 16(1), e0245618.

Benavente-Diaz, M., Comai, G., Di Girolamo, D., Langa, F., & Tajbakhsh, S. (2021). Dynamics of myogenic differentiation using a novel Myogenin knock-in reporter mouse. Skeletal Muscle, 11, 1-13.

Yagi, M., Ji, F., Charlton, J., Cristea, S., Messemer, K., Horwitz, N., ... & Hochedlinger, K. (2021). Dissecting dual roles of MyoD during lineage conversion to mature myocytes and myogenic stem cells. Genes & Development, 35(17-18), 1209-1228.

Ganassi, M., Badodi, S., Wanders, K., Zammit, P. S., & Hughes, S. M. (2020). Myogenin is an essential regulator of adult myofibre growth and muscle stem cell homeostasis. Elife, 9, e60445.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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