Mouse Anti-MYT1L (AA 301-410) Recombinant Antibody (CBFYM-3023) (CBMAB-M3218-FY)

Name: Mouse Anti-MYT1L (AA 301-410) Recombinant Antibody (CBFYM-3023)
SKU: CBMAB-M3218-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYM-3023

Application

ELISA, WB

Immunogen

Recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.Immunogen sequence: MLGKPMNNGL MEKMVEESDE EVCLSSLECL RNQCFDLARK LSETNPQERN PQQNMNIRQH VRPEEDFPGR TPDRNYSDML NLMRLEEQLS PRSRVFASCA KEDGCHERDD

Specificity

Human

Antibody Isotype

IgG1, k

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 301-410

More Infomation

Target

Full Name

myelin transcription factor 1-like

Introduction

This gene encodes a member of the zinc finger superfamily of transcription factors whose expression, thus far, has been found only in neuronal tissues. The encoded protein belongs to a novel class of cystein-cystein-histidine-cystein zinc finger proteins that function in the developing mammalian central nervous system. Forced expression of this gene in combination with the basic helix-loop-helix transcription factor NeuroD1 and the transcription factors POU class 3 homeobox 2 and achaete-scute family basic helix-loop-helix transcription factor 1 can convert fetal and postnatal human fibroblasts into induced neuronal cells, which are able to generate action potentials. Mutations in this gene have been associated with an autosomal dominant form of cognitive disability and with autism spectrum disorder. Alternative splicing results in multiple variants.

Entrez Gene ID

23040

UniProt ID

Q9UL68

Alternative Names

Myelin Transcription Factor 1 Like; Neural Zinc Finger Transcription Factor 1; MyT1-L; Myelin Transcription Factor 1-Like Protein; KIAA1106; ZC2H2C2

Function

Transcription factor that plays a key role in neuronal differentiation by specifically repressing expression of non-neuronal genes during neuron differentiation. In contrast to other transcription repressors that inhibit specific lineages, mediates repression of multiple differentiation programs. Also represses expression of negative regulators of neurogenesis, such as members of the Notch signaling pathway, including HES1. The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro. Directly binds the 5'-AAGTT-3' core motif present on the promoter of target genes and represses transcription by recruiting a multiprotein complex containing SIN3B. The 5'-AAGTT-3' core motif is absent from the promoter of neural genes.

Biological Process

Negative regulation of transcription by RNA polymerase II Source: UniProtKB
Nervous system development Source: UniProtKB
Neuron development Source: UniProtKB
Neuron differentiation Source: UniProtKB
Neuron fate commitment Source: UniProtKB
Neuron fate specification Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: UniProtKB

Cellular Location

Nucleus
Other locations
Chromosome
Note: Preferentially binds to DNA binding sites that are in an open chromatin configuration.

Involvement in disease

Intellectual developmental disorder, autosomal dominant 39 (MRD39):
A form of mental retardation, a disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior and manifested during the developmental period. MRD39 patients show delayed psychomotor development and autistic features.

Fahim, I., Ishaque, A., Ramzan, F., Shamsuddin, S. A. B. A., Ali, A., Salim, A., & Khan, I. (2023). Overexpression of OLIG2 and MYT1L Transcription Factors Enhance the Differentiation Potential of Human Mesenchymal Stem Cells into Oligodendrocytes. Current issues in molecular biology, 45(5), 4100-4123.

Chen, J., Fuhler, N. A., Noguchi, K. K., & Dougherty, J. D. (2023). MYT1L is required for suppressing earlier neuronal development programs in the adult mouse brain. Genome research, 33(4), 541-556.

Xing, L., Chai, R., Wang, J., Lin, J., Li, H., Wang, Y., ... & Chen, G. (2022). Expression of myelin transcription factor 1 and lamin B receptor mediate neural progenitor fate transition in the zebrafish spinal cord pMN domain. Journal of Biological Chemistry, 298(10).

Kim, S., Oh, H., Choi, S. H., Yoo, Y. E., Noh, Y. W., Cho, Y., ... & Kim, E. (2022). Postnatal age-differential ASD-like transcriptomic, synaptic, and behavioral deficits in Myt1l-mutant mice. Cell Reports, 40(12).

Chen, J., Lambo, M. E., Ge, X., Dearborn, J. T., Liu, Y., McCullough, K. B., ... & Dougherty, J. D. (2021). A MYT1L syndrome mouse model recapitulates patient phenotypes and reveals altered brain development due to disrupted neuronal maturation. Neuron, 109(23), 3775-3792.

He, X., Liu, Z., Pang, Y., Xu, W., Zhao, L., & Li, H. (2020). Downregulation of transcription factor TCTP elevates microRNA-200a expression to restrain Myt1L expression, thereby improving neurobehavior and oxidative stress injury in cerebral palsy rats. Cell Cycle, 19(8), 855-869.

Wang, B., Li, D., Yao, Y., Heyns, M., Kovalchuk, A., Ilnytskyy, Y., ... & Kovalchuk, I. (2019). The crucial role of DNA-dependent protein kinase and myelin transcription factor 1-like protein in the miR-141 tumor suppressor network. Cell Cycle, 18(21), 2876-2892.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

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