Mouse Anti-NAA10 Recombinant Antibody (CBT2863) (V2LY-0625-LY628)

Name: Mouse Anti-NAA10 Recombinant Antibody (CBT2863)
SKU: V2LY-0625-LY628
Rating: 5 (1 reviews)

See all NAA10 antibodies

Compare Online Inquiry

Tested Data

Mouse Anti-NAA10 Recombinant Antibody (CBT2863) (V2LY-0625-LY628) in ELISA

Mouse Anti-NAA10 Recombinant Antibody (CBT2863) (V2LY-0625-LY628) in WB

Mouse Anti-NAA10 Recombinant Antibody (CBT2863) (V2LY-0625-LY628) in IF

Mouse Anti-NAA10 Recombinant Antibody (CBT2863) (V2LY-0625-LY628) in FC

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT2863

Application

WB, ICC, FC

Immunogen

Purified recombinant fragment of human NAA10 (AA: 111-235) expressed in E. Coli.

Host Species

Mouse

Specificity

Human, Mouse, Monkey

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
ICC	1:200-1:1,000
FC	1:200-1:400
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

N(Alpha)-Acetyltransferase 10, NatA Catalytic Subunit

Entrez Gene ID

8260

UniProt ID

P41227

Function

Catalytic subunit of N-terminal acetyltransferase complexes which display alpha (N-terminal) acetyltransferase activity (PubMed:15496142, PubMed:19826488, PubMed:19420222, PubMed:20145209, PubMed:27708256, PubMed:25489052, PubMed:29754825, PubMed:20154145, PubMed:32042062).

Acetylates amino termini that are devoid of initiator methionine (PubMed:19420222).

The alpha (N-terminal) acetyltransferase activity may be important for vascular, hematopoietic and neuronal growth and development. Without NAA15, displays epsilon (internal) acetyltransferase activity towards HIF1A, thereby promoting its degradation (PubMed:12464182).

Represses MYLK kinase activity by acetylation, and thus represses tumor cell migration (PubMed:19826488).

Acetylates, and stabilizes TSC2, thereby repressing mTOR activity and suppressing cancer development (PubMed:20145209).

Acetylates HSPA1A and HSPA1B at 'Lys-77' which enhances its chaperone activity and leads to preferential binding to co-chaperone HOPX (PubMed:27708256).

Acetylates HIST1H4A (PubMed:29754825).

Acts as a negative regulator of sister chromatid cohesion during mitosis (PubMed:27422821).

Biological Process

DNA packaging Source: ProtInc
Internal protein amino acid acetylation Source: ProtInc
Negative regulation of maintenance of mitotic sister chromatid cohesion, centromeric Source: UniProtKB
N-terminal peptidyl-glutamic acid acetylation Source: GO_Central
N-terminal peptidyl-serine acetylation Source: GO_Central
N-terminal protein amino acid acetylation Source: UniProtKB
Protein acetylation Source: UniProtKB

Cellular Location

Cytoplasm
Nucleus
Note: Also present in the free cytosolic and cytoskeleton-bound polysomes.

Involvement in disease

N-terminal acetyltransferase deficiency (NATD):
An enzymatic deficiency resulting in postnatal growth failure with severe delays and dysmorphic features. It is clinically characterized by wrinkled forehead, prominent eyes, widely opened anterior and posterior fontanels, downsloping palpebral fissures, thickened lids, large ears, flared nares, hypoplastic alae, short columella, protruding upper lip, and microretrognathia. There are also delayed closing of fontanels and broad great toes. Skin is characterized by redundancy or laxity with minimal subcutaneous fat, cutaneous capillary malformations, and very fine hair and eyebrows. Death results from cardiogenic shock following arrhythmia.
Microphthalmia, syndromic, 1 (MCOPS1):
A rare syndrome defined by the canonical features of unilateral or bilateral microphthalmia or anophthalmia and defects in the skeletal and genitourinary systems. Microphthalmia is a disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. Anomalies of the digits, teeth, and ears are hallmarks of MCOPS1. Intellectual disability ranges from mild to severe, with self-mutilating behaviors and seizures in severely affected MCOPS1 individuals.

PTM

Cleaved by caspases during apoptosis.
Phosphorylation by IKBKB/IKKB at Ser-209 promotes its proteasome-mediated degradation.
Autoacetylated at Lys-136 which stimulates its catalytic activity.

McTiernan, N., Tranebjærg, L., Bjørheim, A. S., Hogue, J. S., Wilson, W. G., Schmidt, B., ... & Arnesen, T. (2022). Biochemical analysis of novel NAA10 variants suggests distinct pathogenic mechanisms involving impaired protein N-terminal acetylation. Human Genetics, 141(8), 1355-1369.

McTiernan, N., Gill, H., Prada, C. E., Pachajoa, H., Lores, J., CAUSES study, & Arnesen, T. (2021). NAA10 p.(N101K) disrupts N-terminal acetyltransferase complex NatA and is associated with developmental delay and hemihypertrophy. European Journal of Human Genetics, 29(2), 280-288.

Vo, T. T. L., Park, J. H., Lee, E. J., Nguyen, Y. T. K., Han, B. W., Nguyen, H. T. T., ... & Seo, J. H. (2020). Characterization of lysine acetyltransferase activity of recombinant human ARD1/NAA10. Molecules, 25(3), 588.

Ree, R., Geithus, A. S., Tørring, P. M., Sørensen, K. P., Damkjær, M., DDD study, ... & Arnesen, T. (2019). A novel NAA10 p.(R83H) variant with impaired acetyltransferase activity identified in two boys with ID and microcephaly. BMC Medical Genetics, 20, 1-8.

Darbakk, C. (2019). Functional characterization of N-terminal acetyltransferase 10 (NAA10) variants potentially causing disease (Master's thesis, The University of Bergen).

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-NAA10 Recombinant Antibody (CBT2863)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Related Products

Mouse Anti-NAA10 Recombinant Antibody (CBT3227) (CAT#: V2LY-0625-LY629)

Mouse Anti-NAA10 Recombinant Antibody (CBWJN-1145) (CAT#: CBMAB-N1007-WJ)

Mouse Anti-NAA10 Recombinant Antibody (CBYC-A1003) (CAT#: CBMAB-A4591-YC)

Rabbit Anti-NAA10 Recombinant Antibody (E1J2B) (CAT#: CBMAB-A4590-YC)

Mouse Anti-NAA10 Recombinant Antibody (CBWJN-0409) (CAT#: CBMAB-N1008-WJ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry