Mouse Anti-NAP1L1 Recombinant Antibody (CBWJN-0307) (CBMAB-N1143-WJ)

Name: Mouse Anti-NAP1L1 Recombinant Antibody (CBWJN-0307)
SKU: CBMAB-N1143-WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBWJN-0307

Application

WB, Dot

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Concentration

0.1 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

nucleosome assembly protein 1-like 1

Introduction

This gene encodes a member of the nucleosome assembly protein (NAP) family. This protein participates in DNA replication and may play a role in modulating chromatin formation and contribute to the regulation of cell proliferation. Alternative splicing results in multiple transcript variants encoding different isoforms; however, not all have been fully described. [provided by RefSeq, Apr 2015]

Entrez Gene ID

Human	4673
Mouse	53605
Rat	89825

UniProt ID

Human	P55209
Mouse	P28656
Rat	Q9Z2G8

Alternative Names

Nucleosome Assembly Protein 1 Like 1; Nucleosome Assembly Protein 1-Like 1; NAP-1-Related Protein; NRP; HSP22-Like Protein Interacting Protein; NAP1L; NAP1; HNRP;

Function

Histone chaperone that plays a role in the nuclear import of H2A-H2B and nucleosome assembly (PubMed:20002496, PubMed:26841755).

Participates also in several important DNA repair mechanisms: greatly enhances ERCC6-mediated chromatin remodeling which is essential for transcription-coupled nucleotide excision DNA repair (PubMed:28369616).

Stimulates also homologous recombination (HR) by RAD51 and RAD54 which is essential in mitotic DNA double strand break (DSB) repair (PubMed:24798879).

Plays a key role in the regulation of embryonic neurogenesis (By similarity).

Promotes the proliferation of neural progenitors and inhibits neuronal differentiation during cortical development (By similarity).

Regulates neurogenesis via the modulation of RASSF10; regulates RASSF10 expression by promoting SETD1A-mediated H3K4 methylation at the RASSF10 promoter (By similarity).

(Microbial infection) Positively regulates Epstein-Barr virus reactivation in epithelial cells through the induction of viral BZLF1 expression.

(Microbial infection) Together with human herpesvirus 8 protein LANA1, assists the proper assembly of the nucleosome on the replicated viral DNA.

Biological Process

DNA replication Source: ProtInc
Nucleosome assembly Source: GO_Central
Positive regulation of cell population proliferation Source: ProtInc
Positive regulation of neural precursor cell proliferation Source: UniProtKB
Positive regulation of neurogenesis Source: UniProtKB

Cellular Location

Cytoplasm
Nucleus
Other locations
Melanosome
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

PTM

Monoglycylated on glutamate residues. Cannot be polyglycylated due to the absence of functional TTLL10 in human (By similarity).
Polyglutamylated by TTLL4 on glutamate residues, resulting in polyglutamate chains on the gamma-carboxyl group. Both polyglutamylation and monoglycylation modifications can coexist on the same protein on adjacent residues, and lowering polyglycylation levels increases polyglutamylation, and reciprocally.

Shen, B., Zhu, W., Liu, X., & Jiang, J. (2022). NAP1L1 Functions as a Novel Prognostic Biomarker Associated With Macrophages and Promotes Tumor Progression by Influencing the Wnt/β-Catenin Pathway in Hepatocellular Carcinoma. Frontiers in Genetics, 13, 876253.

Freitag, M., & Schwertz, H. (2022). A new role of NAP1L1 in megakaryocytes and human platelets. International Journal of Molecular Sciences, 23(23), 14694.

Zhu, X., Xie, Y., Huang, W., Chen, Z., & Guo, S. (2022). NAP1L1 promotes tumor proliferation through HDGF/C-JUN signaling in ovarian cancer. BMC cancer, 22(1), 1-10.

Liu, Y., Li, X., Zhang, Y., Tang, Y., Fang, W., Liu, X., & Liu, Z. (2021). NAP1L1 targeting suppresses the proliferation of nasopharyngeal carcinoma. Biomedicine & Pharmacotherapy, 143, 112096.

Liu, S., Zhang, Y., Cui, S., Song, D., Li, B., Chen, Q., ... & Gong, B. (2021). NAP1L1 interacts with hepatoma-derived growth factor to recruit c-Jun inducing breast cancer growth. Cancer Cell International, 21(1), 1-16.

Chen, Z., Xie, Y., Luo, H., Song, Y., Que, T., Hu, R., ... & Luo, Q. (2021). NAP1L1 promotes proliferation and chemoresistance in glioma by inducing CCND1/CDK4/CDK6 expression through its interaction with HDGF and activation of c-Jun. Aging (Albany NY), 13(24), 26180.

Queiroz, C. J., Song, F., Reed, K. R., Al-Khafaji, N., Clarke, A. R., Vimalachandran, D., ... & Jenkins, J. R. (2020). NAP1L1: a novel human colorectal cancer biomarker derived from animal models of Apc Inactivation. Frontiers in Oncology, 10, 1565.

Nagashio, R., Kuchitsu, Y., Igawa, S., Kusuhara, S., Naoki, K., Satoh, Y., ... & Sato, Y. (2020). Prognostic significance of NAP1L1 expression in patients with early lung adenocarcinoma. Biomedical Research, 41(3), 149-159.

Tanaka, T., Hozumi, Y., Martelli, A. M., Iino, M., & Goto, K. (2019). Nucleosome assembly proteins NAP1L1 and NAP1L4 modulate p53 acetylation to regulate cell fate. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1866(12), 118560.

Le, Y., Kan, A., Li, Q. J., He, M. K., Chen, H. L., & Shi, M. (2019). NAP1L1 is a prognostic biomarker and contribute to doxorubicin chemotherapy resistance in human hepatocellular carcinoma. Cancer cell international, 19(1), 1-11.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
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Isotype control

For research use only. Not intended for any clinical use.

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