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Mouse Anti-NCAPH Recombinant Antibody (1C9) (CBMAB-N1373-WJ)

This product is a Mouse antibody that recognizes NCAPH. The antibody 1C9 can be used for immunoassay techniques such as: ELISA, WB.
See all NCAPH antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1C9
Antibody Isotype
IgG1, κ
Application
ELISA, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Non-SMC Condensin I Complex Subunit H
Introduction
This gene encodes a member of the barr gene family and a regulatory subunit of the condensin complex. This complex is required for the conversion of interphase chromatin into condensed chromosomes. The protein encoded by this gene is associated with mitotic chromosomes, except during the early phase of chromosome condensation. During interphase, the protein has a distinct punctate nucleolar localization. Alternatively spliced transcript variants encoding different proteins have been described. [provided by RefSeq, Jul 2013]
Entrez Gene ID
23397
UniProt ID
Q15003
Alternative Names
Non-SMC Condensin I Complex Subunit H; Chromosome-Associated Protein H; BRRN1; Non-SMC Condensin I Complex, Subunit H; Barren Homolog 1 (Drosophila); Barren (Drosophila) Homolog; Barren Homolog (Drosophila); Condensin Complex Subunit 2; Barren Homolog Protein 1;
Function
Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases (PubMed:11136719).

Early in neurogenesis, may play an essential role to ensure accurate mitotic chromosome condensation in neuron stem cells, ultimately affecting neuron pool and cortex size (PubMed:27737959).
Biological Process
Cell division Source: UniProtKB-KW
Female meiosis chromosome separation Source: Ensembl
Meiotic chromosome condensation Source: Ensembl
Mitotic chromosome condensation Source: UniProtKB
Positive regulation of chromosome condensation Source: ComplexPortal
Positive regulation of chromosome segregation Source: Ensembl
Positive regulation of chromosome separation Source: Ensembl
Cellular Location
Cytoplasm
Nucleus
Other locations
Chromosome
Note: In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDK1, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase.
Involvement in disease
Microcephaly 23, primary, autosomal recessive (MCPH23):
A form of microcephaly, a disease defined as a head circumference more than 3 standard deviations below the age, sex and ethnically matched mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small.
PTM
Phosphorylated by CDK1. Its phosphorylation, as well as that of NCAPD2 and NCAPG subunits, activates the condensin complex and is required for chromosome condensation (By similarity).

Li, B., Xiao, Q., Shan, L., & Song, Y. (2022). NCAPH promotes cell proliferation and inhibits cell apoptosis of bladder cancer cells through MEK/ERK signaling pathway. Cell Cycle, 21(4), 427-438.

Ogura, T., Azuma, K., Sato, J., Kinowaki, K., Takayama, K. I., Takeiwa, T., ... & Inoue, S. (2021). OCT1 is a poor prognostic factor for breast cancer patients and promotes cell proliferation via inducing NCAPH. International Journal of Molecular Sciences, 22(21), 11505.

Xiong, Q., Jiang, L., Liu, K., Jiang, X., Liu, B., Shi, Y., ... & Chen, Y. (2021). miR-133b targets NCAPH to promote β-catenin degradation and reduce cancer stem cell maintenance in non-small cell lung cancer. Signal Transduction and Targeted Therapy, 6(1), 252.

Xiong, Y. C., Wang, J., Cheng, Y., Zhang, X. Y., & Ye, X. Q. (2020). Overexpression of MYBL2 promotes proliferation and migration of non-small-cell lung cancer via upregulating NCAPH. Molecular and Cellular Biochemistry, 468, 185-193.

Wang, M., Qiao, X., Cooper, T., Pan, W., Liu, L., Hayball, J., ... & Wang, X. (2020). HPV E7-mediated NCAPH ectopic expression regulates the carcinogenesis of cervical carcinoma via PI3K/AKT/SGK pathway. Cell Death & Disease, 11(12), 1049.

Lu, H., Shi, C., Wang, S., Yang, C., Wan, X., Luo, Y., ... & Li, L. (2020). Identification of NCAPH as a biomarker for prognosis of breast cancer. Molecular biology reports, 47, 7831-7842.

Kim, B., Kim, S. W., Lim, J. Y., & Park, S. J. (2020). NCAPH is required for proliferation, migration and invasion of non-small-cell lung cancer cells. Anticancer Research, 40(6), 3239-3246.

Qiu, X., Gao, Z., Shao, J., & Li, H. (2020). NCAPH is upregulated in endometrial cancer and associated with poor clinicopathologic characteristics. Annals of human genetics, 84(6), 437-446.

Cui, F., Hu, J., Xu, Z., Tan, J., & Tang, H. (2019). Overexpression of NCAPH is upregulated and predicts a poor prognosis in prostate cancer. Oncology letters, 17(6), 5768-5776.

Shimomura, H., Sasahira, T., Nakashima, C., Kurihara-Shimomura, M., & Kirita, T. (2019). Non-SMC condensin I complex subunit H (NCAPH) is associated with lymphangiogenesis and drug resistance in oral squamous cell carcinoma. Journal of Clinical Medicine, 9(1), 72.

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For research use only. Not intended for any clinical use.

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