Mouse Anti-NDE1 Recombinant Antibody (1F7) (CBMAB-N0205-WJ)

This product is a Mouse antibody that recognizes NDE1. The antibody 1F7 can be used for immunoassay techniques such as: WB, ELISA.
See all NDE1 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

1F7

Antibody Isotype

IgG1, κ

Application

WB, ELISA

Basic Information

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Concentration

1 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

nudE nuclear distribution gene E homolog 1 (A. nidulans)

Introduction

This gene encodes a member of the nuclear distribution E (NudE) family of proteins. The encoded protein is localized at the centrosome and interacts with other centrosome components as part of a multiprotein complex that regulates dynein function. This protein plays an essential role in microtubule organization, mitosis and neuronal migration. Mutations in this gene cause lissencephaly 4, a disorder characterized by lissencephaly, severe brain atrophy, microcephaly, and severe cognitive disability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2012]

Entrez Gene ID

54820

UniProt ID

Q9NXR1

Alternative Names

NudE Neurodevelopment Protein 1; NUDE; NudE Nuclear Distribution Gene E Homolog 1 (A. Nidulans); NudE Nuclear Distribution E Homolog 1 (A. Nidulans); LIS1-Interacting Protein NUDE1, Rat Homolog; Nuclear Distribution Protein NudE Homolog 1; NudE Nuclear Distribution Gene E Homolog 1;

Function

Required for centrosome duplication and formation and function of the mitotic spindle. Essential for the development of the cerebral cortex. May regulate the production of neurons by controlling the orientation of the mitotic spindle during division of cortical neuronal progenitors of the proliferative ventricular zone of the brain. Orientation of the division plane perpendicular to the layers of the cortex gives rise to two proliferative neuronal progenitors whereas parallel orientation of the division plane yields one proliferative neuronal progenitor and a post-mitotic neuron. A premature shift towards a neuronal fate within the progenitor population may result in an overall reduction in the final number of neurons and an increase in the number of neurons in the deeper layers of the cortex.

Biological Process

Cell division Source: UniProtKB-KW
Cell migration Source: GO_Central
Centrosome duplication Source: UniProtKB
Centrosome localization Source: GO_Central
Cerebral cortex development Source: UniProtKB
Chromosome segregation Source: GO_Central
Establishment of chromosome localization Source: UniProtKB
Establishment of mitotic spindle orientation Source: UniProtKB
Microtubule nucleation Source: GO_Central
Mitotic centrosome separation Source: GO_Central
Neuroblast proliferation Source: Ensembl
Neuron migration Source: Ensembl
Vesicle transport along microtubule Source: GO_Central

Cellular Location

Cytoskeleton
Centrosome
Spindle
Other locations
kinetochore
Cleavage furrow
Note: Localizes to the interphase and S phase centrosome. During mitosis, partially associated with the mitotic spindle. Concentrates at the plus ends of microtubules coincident with kinetochores in metaphase and anaphase in a CENPF-dependent manner. Also localizes to the cleavage furrow during cytokinesis. manner. Also localizes to the cleavage furrow during cytokinesis.

Involvement in disease

Lissencephaly 4 (LIS4):
A neurodevelopmental disorder characterized by lissencephaly, severe brain atrophy, extreme microcephaly, and profound mental retardation.
Microhydranencephaly (MHAC):
A severe neurodevelopmental disorder characterized by microcephaly, severe motor and mental retardation, spasticity, and brain malformations that include gross dilation of the ventricles with complete absence of the cerebral hemispheres or severe delay in their development.

PTM

Phosphorylated in mitosis. Phosphorylated in vitro by CDC2. Phosphorylation at Thr-246 is essential for the G2/M transition (By similarity).

References

Tsai, M. H., Ke, H. C., Lin, W. C., Nian, F. S., Huang, C. W., Cheng, H. Y., ... & Tsai, J. W. (2024). Novel lissencephaly-associated NDEL1 variant reveals distinct roles of NDE1 and NDEL1 in nucleokinesis and human cortical malformations. Acta Neuropathologica, 147(1), 13.

Zhang, Q., Li, T., Lin, J., Zhang, Y., Li, F., Chen, X., ... & Li, Q. (2022). Deficiency of nde1 in zebrafish induces brain inflammatory responses and autism-like behavior. IScience, 25(3).

Chomiak, A. A., Guo, Y., Kopsidas, C. A., McDaniel, D. P., Lowe, C. C., Pan, H., ... & Feng, Y. (2022). Nde1 is required for heterochromatin compaction and stability in neocortical neurons. Iscience, 25(6), 104354.

Bas, H., Saylisoy, S., Cilingir, O., Gokalp, E. E., Kocagil, S., Yarar, C., ... & Artan, S. (2022). NDE1‐related disorders: A recurrent NDE1 pathogenic variant causing Lissencephaly 4 can also be associated with microhydranencephaly. American Journal of Medical Genetics Part A, 188(1), 326-331.

Soto-Perez, J., Baumgartner, M., & Kanadia, R. N. (2020). Role of NDE1 in the Development and Evolution of the Gyrified Cortex. Frontiers in Neuroscience, 14, 617513.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

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Mouse Anti-NDE1 Recombinant Antibody (2G11-1C11)

Mouse Anti-NDE1 Recombinant Antibody (CBWJN-0343)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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