Mouse Anti-NDFIP2 Recombinant Antibody (2C7) (CBMAB-N1550-WJ)

Name: Mouse Anti-NDFIP2 Recombinant Antibody (2C7)
SKU: CBMAB-N1550-WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

2C7

Application

ELISA, WB

Specificity

Human

Antibody Isotype

IgG2a, κ

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.2

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Nedd4 family interacting protein 2

Introduction

Activates HECT domain-containing E3 ubiquitin-protein ligases, including ITCH, NEDD4, NEDD4L, SMURF2, WWP1 and WWP2, and consequently modulates the stability of their targets. As a result, may control many cellular processes. Recruits ITCH, NEDD4 and SMURF2 to endosomal membranes. Negatively regulates KCNH2 potassium channel activity by decreasing its cell-surface expression and interfering with channel maturation through recruitment of NEDD4L to the Golgi apparatus and multivesicular body where it mediates KCNH2 degradation (PubMed:26363003). May modulate EGFR signaling. Together with NDFIP1, limits the cytokine signaling and expansion of effector Th2 T-cells by promoting degradation of JAK1, probably by ITCH- and NEDD4L-mediated ubiquitination (By similarity).

Entrez Gene ID

54602

UniProt ID

Q9NV92

Alternative Names

Nedd4 Family Interacting Protein 2; Putative MAPK-Activating Protein PM04/PM05/PM06/PM07; Putative NF-Kappa-B-Activating Protein 413; NEDD4 WW Domain-Binding Protein 5A; N4WBP5A; MAPK-Activating Protein PM04 PM05 PM06 PM07; NEDD4 Family-Interacting Protein 2; NF-Kappa-B-Activating Protein 413; KIAA1165;

Function

Activates HECT domain-containing E3 ubiquitin-protein ligases, including ITCH, NEDD4, NEDD4L, SMURF2, WWP1 and WWP2, and consequently modulates the stability of their targets. As a result, may control many cellular processes. Recruits ITCH, NEDD4 and SMURF2 to endosomal membranes. Negatively regulates KCNH2 potassium channel activity by decreasing its cell-surface expression and interfering with channel maturation through recruitment of NEDD4L to the Golgi apparatus and multivesicular body where it mediates KCNH2 degradation (PubMed:26363003).

May modulate EGFR signaling. Together with NDFIP1, limits the cytokine signaling and expansion of effector Th2 T-cells by promoting degradation of JAK1, probably by ITCH- and NEDD4L-mediated ubiquitination (By similarity).

Biological Process

Metal ion transport Source: GO_Central
Negative regulation of gene expression Source: UniProtKB
Negative regulation of protein transport Source: UniProtKB
Negative regulation of transporter activity Source: UniProtKB
Positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
Positive regulation of protein ubiquitination Source: UniProtKB
Ubiquitin-dependent protein catabolic process Source: GO_Central
Vacuolar transport Source: InterPro

Cellular Location

Endosome
Endosome membrane
multivesicular body membrane
Golgi apparatus
Golgi apparatus membrane

Topology

Cytoplasmic: 1-231
Helical: 232-252
Extracellular: 253-257
Helical: 258-278
Cytoplasmic: 279-287
Helical: 288-308
Extracellular: 309-336

PTM

Ubiquitinated by NEDD4 and ITCH. Also ubiquitinated by NEDD4L. Ubiquitination by NEDD4 or NEDD4L does not affect turnover (By similarity).
Undergoes transient tyrosine-phosphorylation following EGF stimulation, most probably catalyzed by SRC. Phosphorylation on Tyr-151, Tyr-171 and Tyr-177 are dependent on the phosphorylation on Tyr-167. Also phosphorylated by LYN and FYN.

Marziali, F., Song, Y., Nguyen, X. N., Belmudes, L., Burlaud-Gaillard, J., Roingeard, P., ... & Cimarelli, A. (2023). A Proteomics-Based Approach Identifies the NEDD4 Adaptor NDFIP2 as an Important Regulator of Ifitm3 Levels. Viruses, 15(10), 1993.

Zhou, Y., Hu, J., Liu, L., Yan, M., Zhang, Q., Song, X., ... & Li, X. (2021). Gly-tRF enhances LCSC-like properties and promotes HCC cells migration by targeting NDFIP2. Cancer Cell International, 21, 1-16.

Choi, E. H., & Hwang, D. (2021). Association Study of NDFIP2 Genetic Polymorphism with Asthma in the Korean Population. Korean Journal of Clinical Laboratory Science, 53(3), 249-256.

Zhou, Y., Hu, J., Liu, L., Yan, M., Zhang, Q., Song, X., ... & Li, X. (2021). Gly-tRF Enhances LCSCs-Like Cells Stemness and Promotes EMT of HCC Cells via Targeting NDFIP2 and Activating AKT Signaling Pathway.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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