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Mouse Anti-NDFIP2 Recombinant Antibody (2C7) (CBMAB-N1550-WJ)

This product is a Mouse antibody that recognizes NDFIP2. The antibody 2C7 can be used for immunoassay techniques such as: ELISA, WB.
See all NDFIP2 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2C7
Antibody Isotype
IgG2a, κ
Application
ELISA, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG2a, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Nedd4 family interacting protein 2
Introduction
Activates HECT domain-containing E3 ubiquitin-protein ligases, including ITCH, NEDD4, NEDD4L, SMURF2, WWP1 and WWP2, and consequently modulates the stability of their targets. As a result, may control many cellular processes. Recruits ITCH, NEDD4 and SMURF2 to endosomal membranes. Negatively regulates KCNH2 potassium channel activity by decreasing its cell-surface expression and interfering with channel maturation through recruitment of NEDD4L to the Golgi apparatus and multivesicular body where it mediates KCNH2 degradation (PubMed:26363003). May modulate EGFR signaling. Together with NDFIP1, limits the cytokine signaling and expansion of effector Th2 T-cells by promoting degradation of JAK1, probably by ITCH- and NEDD4L-mediated ubiquitination (By similarity).
Entrez Gene ID
54602
UniProt ID
Q9NV92
Alternative Names
Nedd4 Family Interacting Protein 2; Putative MAPK-Activating Protein PM04/PM05/PM06/PM07; Putative NF-Kappa-B-Activating Protein 413; NEDD4 WW Domain-Binding Protein 5A; N4WBP5A; MAPK-Activating Protein PM04 PM05 PM06 PM07; NEDD4 Family-Interacting Protein 2; NF-Kappa-B-Activating Protein 413; KIAA1165;
Function
Activates HECT domain-containing E3 ubiquitin-protein ligases, including ITCH, NEDD4, NEDD4L, SMURF2, WWP1 and WWP2, and consequently modulates the stability of their targets. As a result, may control many cellular processes. Recruits ITCH, NEDD4 and SMURF2 to endosomal membranes. Negatively regulates KCNH2 potassium channel activity by decreasing its cell-surface expression and interfering with channel maturation through recruitment of NEDD4L to the Golgi apparatus and multivesicular body where it mediates KCNH2 degradation (PubMed:26363003).

May modulate EGFR signaling. Together with NDFIP1, limits the cytokine signaling and expansion of effector Th2 T-cells by promoting degradation of JAK1, probably by ITCH- and NEDD4L-mediated ubiquitination (By similarity).
Biological Process
Metal ion transport Source: GO_Central
Negative regulation of gene expression Source: UniProtKB
Negative regulation of protein transport Source: UniProtKB
Negative regulation of transporter activity Source: UniProtKB
Positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
Positive regulation of protein ubiquitination Source: UniProtKB
Ubiquitin-dependent protein catabolic process Source: GO_Central
Vacuolar transport Source: InterPro
Cellular Location
Endosome
Endosome membrane
multivesicular body membrane
Golgi apparatus
Golgi apparatus membrane
Topology
Cytoplasmic: 1-231
Helical: 232-252
Extracellular: 253-257
Helical: 258-278
Cytoplasmic: 279-287
Helical: 288-308
Extracellular: 309-336
PTM
Ubiquitinated by NEDD4 and ITCH. Also ubiquitinated by NEDD4L. Ubiquitination by NEDD4 or NEDD4L does not affect turnover (By similarity).
Undergoes transient tyrosine-phosphorylation following EGF stimulation, most probably catalyzed by SRC. Phosphorylation on Tyr-151, Tyr-171 and Tyr-177 are dependent on the phosphorylation on Tyr-167. Also phosphorylated by LYN and FYN.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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