Mouse Anti-NDUFS2 Recombinant Antibody (7A12BE5AD5) (CBMAB-N1678-WJ)

Name: Mouse Anti-NDUFS2 Recombinant Antibody (7A12BE5AD5)
SKU: CBMAB-N1678-WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

7A12BE5AD5

Application

WB, FC, ICC

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Concentration

1 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

NADH:Ubiquinone Oxidoreductase Core Subunit S2

Introduction

The protein encoded by this gene is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (complex I). Mammalian mitochondrial complex I is composed of at least 43 different subunits, 7 of which are encoded by the mitochondrial genome, and the rest are the products of nuclear genes. The iron-sulfur protein fraction of complex I is made up of 7 subunits, including this gene product. Complex I catalyzes the NADH oxidation with concomitant ubiquinone reduction and proton ejection out of the mitochondria. Mutations in this gene are associated with mitochondrial complex I deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]

Entrez Gene ID

4720

UniProt ID

O75306

Alternative Names

NADH:Ubiquinone Oxidoreductase Core Subunit S2; NADH Dehydrogenase (Ubiquinone) Fe-S Protein 2, 49kDa (NADH-Coenzyme Q Reductase); NADH Dehydrogenase [Ubiquinone] Iron-Sulfur Protein 2, Mitochondrial; NADH-Ubiquinone Oxidoreductase 49 KDa Subunit; Complex I 49kDa Subunit; Complex I-49kD; CI-49kD; NADH Dehydrogenase (Ubiquinone) Fe-S Protein 2 (49kD) (NADH-Coenzyme Q Reductase);

Function

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (PubMed:30922174, PubMed:22036843).

Essential for the catalytic activity of complex I (PubMed:30922174, PubMed:22036843).

Essential for the assembly of complex I (By similarity).

Redox-sensitive, critical component of the oxygen-sensing pathway in the pulmonary vasculature which plays a key role in acute pulmonary oxygen-sensing and hypoxic pulmonary vasoconstriction (PubMed:30922174).

Plays an important role in carotid body sensing of hypoxia (By similarity).

Essential for glia-like neural stem and progenitor cell proliferation, differentiation and subsequent oligodendrocyte or neuronal maturation (By similarity).

Biological Process

Aerobic respiration Source: ComplexPortal
Cellular response to oxygen levels Source: UniProtKB
Gliogenesis Source: UniProtKB
Mitochondrial ATP synthesis coupled electron transport Source: CAFA
Mitochondrial ATP synthesis coupled proton transport Source: ComplexPortal
Mitochondrial electron transport, NADH to ubiquinone Source: UniProtKB
Mitochondrial respiratory chain complex I assembly Source: UniProtKB
Neural precursor cell proliferation Source: UniProtKB
Neurogenesis Source: UniProtKB
Response to oxidative stress Source: UniProtKB

Cellular Location

Mitochondrion inner membrane

Involvement in disease

Mitochondrial complex I deficiency, nuclear type 6 (MC1DN6):
A form of mitochondrial complex I deficiency, the most common biochemical signature of mitochondrial disorders, a group of highly heterogeneous conditions characterized by defective oxidative phosphorylation, which collectively affects 1 in 5-10000 live births. Clinical disorders have variable severity, ranging from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. MC1DN6 transmission pattern is consistent with autosomal recessive inheritance.

PTM

Dimethylation at Arg-118 by NDUFAF7 takes place after NDUFS2 assembles into the complex I, leading to stabilize the early intermediate complex (PubMed:24089531, PubMed:24838397).

McElroy, G. S., Chakrabarty, R. P., D’Alessandro, K. B., Hu, Y. S., Vasan, K., Tan, J., ... & Chandel, N. S. (2022). Reduced expression of mitochondrial complex I subunit Ndufs2 does not impact healthspan in mice. Scientific reports, 12(1), 5196.

Bandara, A. B., Drake, J. C., James, C. C., Smyth, J. W., & Brown, D. A. (2021). Complex I protein NDUFS2 is vital for growth, ROS generation, membrane integrity, apoptosis, and mitochondrial energetics. Mitochondrion, 58, 160-168.

Yang, Y., Yang, X., Lin, Y., Yang, G., & Li, L. (2020). LASS2 regulates hepatocyte steatosis by interacting with NDUFS2/OXPHOS related proteins. Biochemical and Biophysical Research Communications, 526(4), 871-879.

Liu, L., Qi, L., Knifley, T., Piecoro, D. W., Rychahou, P., Liu, J., ... & Chen, M. (2019). S100A4 alters mitochondrial metabolism to promote invasion and metastasis of non-small cell lung cancer cells through upregulation of NDUFS2. Cancer Research, 79(13_Supplement), 1119-1119.

Wolin, M. S., Alruwaili, N., & Kandhi, S. (2019). Studies on hypoxic pulmonary vasoconstriction detect a novel role for the mitochondrial complex I subunit Ndufs2 in controlling peroxide generation for oxygen-sensing. Circulation research, 124(12), 1683-1685.

Liu, L., Qi, L., Knifley, T., Piecoro, D. W., Rychahou, P., Liu, J., ... & Chen, M. (2019). S100A4 alters metabolism and promotes invasion of lung cancer cells by up-regulating mitochondrial complex I protein NDUFS2. Journal of Biological Chemistry, 294(18), 7516-7527.

Dunham-Snary, K. J., Wu, D., Potus, F., Sykes, E. A., Mewburn, J. D., Charles, R. L., ... & Archer, S. L. (2019). Ndufs2, a core subunit of mitochondrial complex I, is essential for acute oxygen-sensing and hypoxic pulmonary vasoconstriction. Circulation research, 124(12), 1727-1746.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
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For research use only. Not intended for any clinical use.

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