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Mouse Anti-NEDD4L Antibody (2B4) (CBMAB-0711-YC)

Provided herein are mouse monoclonal antibodies against Human NEDD4L. The antibody clone 2B4 can be used for immunoassay techniques, such as IP and MA.
See all NEDD4L antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2B4
Antibody Isotype
IgG2b
Application
IP, MA

Basic Information

Immunogen
Recombinant protein
Specificity
Human
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Supernatant
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
NEDD4L
Introduction
NEDD4L (E3 ubiquitin-protein ligase NEDD4-like) is a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. NEDD4L mediates the ubiquitination of multiple target substrates and plays a critical role in epithelial sodium transport by regulating the cell surface expression of the epithelial sodium channel, ENaC. Single nucleotide polymorphisms in NEDD4L gene may be associated with essential hypertension.
Entrez Gene ID
23327
UniProt ID
Q96PU5
Alternative Names
RSP5; NEDD4-2; NEDD4.2; hNEDD4-2
Function
E3 ubiquitin-protein ligase that mediates the polyubiquitination of lysine and cysteine residues on target proteins and is thereby implicated in the regulation of various signaling pathways including autophagy, innate immunity or DNA repair (PubMed:31959741, PubMed:33608556, PubMed:20064473).

Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation (PubMed:15496141).

Downregulates autophagy and cell growth by ubiquitinating and reducing cellular ULK1 or ASCT2 levels (PubMed:28820317, PubMed:31959741).

Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, SCN2A/Nav1.2, SCN3A/Nav1.3, SCN5A/Nav1.5, SCN9A/Nav1.7, SCN10A/Nav1.8, KCNA3/Kv1.3, KCNH2, EAAT1, KCNQ2/Kv7.2, KCNQ3/Kv7.3 or CLC5 (PubMed:26363003, PubMed:27445338).

Promotes ubiquitination and degradation of SGK1 and TNK2. Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1 (PubMed:25631046).

Plays a role in dendrite formation by melanocytes (PubMed:23999003).

Involved in the regulation of TOR signaling (PubMed:27694961).

Ubiquitinates and regulates protein levels of NTRK1 once this one is activated by NGF (PubMed:27445338).

Plays a role in antiviral innate immunity by catalyzing 'Lys-29'-linked cysteine ubiquitination of TRAF3, resulting in enhanced 'Lys-48' and 'Lys-63'-linked ubiquitination of TRAF3 (PubMed:33608556).
Biological Process
Cell differentiation Source: UniProtKB-KW
Ion transmembrane transport Source: Reactome
Negative regulation of potassium ion transmembrane transport Source: BHF-UCL
Negative regulation of potassium ion transmembrane transporter activity Source: BHF-UCL
Negative regulation of protein localization to cell surface Source: BHF-UCL
Negative regulation of sodium ion transmembrane transport Source: BHF-UCL
Negative regulation of sodium ion transmembrane transporter activity Source: BHF-UCL
Positive regulation of caveolin-mediated endocytosis Source: BHF-UCL
Positive regulation of dendrite extension Source: UniProtKB
Positive regulation of protein catabolic process Source: GO_Central
Proteasome-mediated ubiquitin-dependent protein catabolic process Source: BHF-UCL
Protein K48-linked ubiquitination Source: BHF-UCL
Protein polyubiquitination Source: GO_Central
Protein ubiquitination Source: UniProtKB
Regulation of dendrite morphogenesis Source: GO_Central
Regulation of ion transmembrane transport Source: BHF-UCL
Regulation of membrane depolarization Source: BHF-UCL
Regulation of membrane potential Source: BHF-UCL
Regulation of membrane repolarization Source: BHF-UCL
Regulation of potassium ion transmembrane transporter activity Source: BHF-UCL
Regulation of protein stability Source: UniProtKB
Regulation of sodium ion transmembrane transport Source: UniProtKB
Ubiquitin-dependent protein catabolic process Source: BHF-UCL
Ventricular cardiac muscle cell action potential Source: BHF-UCL
Cellular Location
Cytoplasm
Endosome
multivesicular body
Golgi apparatus
Note: May be recruited to exosomes by NDFIP1.
Involvement in disease
Periventricular nodular heterotopia 7 (PVNH7):
A form of periventricular nodular heterotopia, a disorder resulting from a defect in the pattern of neuronal migration in which ectopic collections of neurons lie along the lateral ventricles of the brain or just beneath, contiguously or in isolated patches. PVNH7 is an autosomal dominant disease characterized by delayed psychomotor development, intellectual disability, and seizures in some patients. Additional features include cleft palate and toe syndactyly.
PTM
Phosphorylated by SGK1 or PKA; which impairs interaction with SCNN. Interaction with YWHAH inhibits dephosphorylation.
Auto-ubiquitinated (PubMed:19343052). Deubiquitinated by USP36, no effect on NEDD4L protein levels. Both proteins interact and regulate each other's ubiquitination levels (PubMed:27445338).

Alrosan, A. Z., Alrosan, K., Heilat, G. B., Alsharedeh, R., Abudalo, R., Oqal, M., ... & Elmaghrabi, Y. A. (2023). Potential roles of NEDD4 and NEDD4L and their utility as therapeutic targets in high‑incidence adult male cancers. Molecular and Clinical Oncology, 19(3), 1-9.

Huang, X., Cao, W., Yao, S., Chen, J., Liu, Y., Qu, J., ... & Cai, Z. (2022). NEDD4L binds the proteasome and promotes autophagy and bortezomib sensitivity in multiple myeloma. Cell Death & Disease, 13(3), 197.

Lin, L., Wu, X., Jiang, Y., Deng, C., Luo, X., Han, J., ... & Cao, X. (2022). Down-regulated NEDD4L facilitates tumor progression through activating Notch signaling in lung adenocarcinoma. PeerJ, 10, e13402.

Li, M., Sun, G., Wang, P., Wang, W., Cao, K., Song, C., ... & Zhang, N. (2022). Research progress of Nedd4L in cardiovascular diseases. Cell Death Discovery, 8(1), 206.

Wang, J., He, Z., Liu, X., Xu, J., Jiang, X., Quan, G., & Jiang, J. (2022). LINC00941 promotes pancreatic cancer malignancy by interacting with ANXA2 and suppressing NEDD4L-mediated degradation of ANXA2. Cell Death & Disease, 13(8), 718.

Xie, S., Xia, L., Song, Y., Liu, H., Wang, Z. W., & Zhu, X. (2021). Insights into the biological role of NEDD4L E3 ubiquitin ligase in human cancers. Frontiers in Oncology, 11, 774648.

Lee, D. E., Yoo, J. E., Kim, J., Kim, S., Kim, S., Lee, H., & Cheong, H. (2020). NEDD4L downregulates autophagy and cell growth by modulating ULK1 and a glutamine transporter. Cell death & disease, 11(1), 38.

Wang, Y., Liu, Y., Liu, J., Kang, R., & Tang, D. (2020). NEDD4L-mediated LTF protein degradation limits ferroptosis. Biochemical and Biophysical Research Communications, 531(4), 581-587.

Wang, X., Duan, J., Fu, W., Yin, Z., Sheng, J., Lei, Z., & Wang, H. (2019). Decreased expression of NEDD4L contributes to NSCLC progression and metastasis. Biochemical and biophysical research communications, 513(2), 398-404.

Jiang, X., Zhang, S., Yin, Z., Sheng, Y., Yan, Q., Sun, R., ... & Li, Y. (2019). The correlation between NEDD4L and HIF-1α levels as a gastric cancer prognostic marker. International Journal of Medical Sciences, 16(11), 1517.

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For research use only. Not intended for any clinical use.

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