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Mouse Anti-NFKBIA Recombinant Antibody (6A920) (CBMAB-I1543-YY)

This product is Mouse antibody that recognizes NFKBIA. The antibody 6A920 can be used for immunoassay techniques such as: ELISA, FC, IHC, IF, IP, IHC-P, CyTOF, WB
See all NFKBIA antibodies
Published Data
Fig.1 Total protein from control or LPS-treated RAW264.7 cells separated on phospho-purification column was immunoprecipitated with Ikba antibody and immunoblotted with UBC9 antibody.
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Summary

Host Animal
Mouse
Specificity
Mouse, Rat, Hamster, Human, Monkey
Clone
6A920
Antibody Isotype
IgG1, κ
Application
ELISA, FC, IHC, IF, IP, IHC-P, CyTOF, WB

Basic Information

Specificity
Mouse, Rat, Hamster, Human, Monkey
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
NFKB Inhibitor Alpha
Introduction
This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease.
Entrez Gene ID
Human4792
Mouse18035
Rat25493
Monkey695335
Hamster100754153
UniProt ID
HumanP25963
MouseQ9Z1E3
RatQ63746
MonkeyH9FUW3
HamsterA0A1U7QWG7
Alternative Names
NFKB Inhibitor Alpha; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells Inhibitor, Alpha; Major Histocompatibility Complex Enhancer-Binding Protein MAD3; NF-Kappa-B Inhibitor Alpha; I-Kappa-B-Alpha; IkappaBalpha; IkB-Alpha;
Function
Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and pro-inflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
Biological Process
Apoptotic processManual Assertion Based On ExperimentTAS:ProtInc
Cellular response to cold1 PublicationNAS:BHF-UCL
Cytoplasmic sequestering of NF-kappaBManual Assertion Based On ExperimentIMP:BHF-UCL
Cytoplasmic sequestering of transcription factorManual Assertion Based On ExperimentIDA:UniProtKB
I-kappaB kinase/NF-kappaB signalingManual Assertion Based On ExperimentIDA:CAFA
Lipopolysaccharide-mediated signaling pathwayIEA:Ensembl
Negative regulation of DNA binding1 PublicationNAS:UniProtKB
Negative regulation of lipid storageManual Assertion Based On ExperimentIMP:BHF-UCL
Negative regulation of macrophage derived foam cell differentiationManual Assertion Based On ExperimentIMP:BHF-UCL
Negative regulation of myeloid cell differentiationIEA:Ensembl
Negative regulation of NF-kappaB transcription factor activityManual Assertion Based On ExperimentIDA:MGI
Negative regulation of Notch signaling pathwayIEA:Ensembl
Nucleotide-binding oligomerization domain containing 1 signaling pathwayIEA:Ensembl
Nucleotide-binding oligomerization domain containing 2 signaling pathwayIEA:Ensembl
Positive regulation of cellular protein metabolic processManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of cholesterol effluxManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of inflammatory responseManual Assertion Based On ExperimentIDA:CAFA
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:UniProtKB
Protein import into nucleusIEA:Ensembl
Regulation of cell population proliferationIEA:Ensembl
Regulation of NIK/NF-kappaB signaling1 PublicationNAS:UniProtKB
Response to exogenous dsRNAIEA:Ensembl
Response to muramyl dipeptideIEA:Ensembl
Response to muscle stretchIEA:Ensembl
Toll-like receptor 4 signaling pathwayIEA:Ensembl
Tumor necrosis factor-mediated signaling pathwayManual Assertion Based On ExperimentIDA:CAFA
Cellular Location
Cytoplasm
Nucleus
Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.
Involvement in disease
Ectodermal dysplasia and immunodeficiency 2 (EDAID2):
A form of ectoderma dysplasia, a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. This form of ectodermal dysplasia is associated with decreased production of pro-inflammatory cytokines and certain interferons, rendering patients susceptible to infection. EDAID2 inheritance is autosomal dominant.
PTM
Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation.
Sumoylated; sumoylation requires the presence of the nuclear import signal. Sumoylation blocks ubiquitination and proteasome-mediated degradation of the protein thereby increasing the protein stability.
Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitination leads to protein degradation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36.
Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interferes with NFKBIA degradation and impairs subsequent NF-kappa-B activation.

Zhu, W., Yu, Y., Ye, Y., Tu, X., Zhang, Y., Wu, T., ... & Cui, R. (2023). MiR-196b-5p activates NF-κB signaling in non-small cell lung cancer by directly targeting NFKBIA. Translational Oncology, 37, 101755.

Bredel, M., Espinosa, L., Kim, H., Scholtens, D. M., McElroy, J. P., Rajbhandari, R., ... & Chakravarti, A. (2023). Haploinsufficiency of NFKBIA reshapes the epigenome antipodal to the IDH mutation and imparts disease fate in diffuse gliomas. Cell Reports Medicine, 4(6).

Yang, W., Li, J., Zhang, M., Yu, H., Zhuang, Y., Zhao, L., ... & Li, X. (2022). Elevated expression of the rhythm gene NFIL3 promotes the progression of TNBC by activating NF-κB signaling through suppression of NFKBIA transcription. Journal of Experimental & Clinical Cancer Research, 41(1), 67.

Chen, M., Liang, X., Liang, Z., & Zhao, L. (2021). Study on the effect and mechanism of NFKBIA on cervical cancer progress in vitro and in vivo. Journal of Obstetrics and Gynaecology Research, 47(11), 3931-3942.

Kolesnichenko, M., Mikuda, N., Höpken, U. E., Kärgel, E., Uyar, B., Tufan, A. B., ... & Scheidereit, C. (2021). Transcriptional repression of NFKBIA triggers constitutive IKK‐and proteasome‐independent p65/RelA activation in senescence. The EMBO journal, 40(6), e104296.

Lu, J., Wang, Y. H., Huang, X. Y., Xie, J. W., Wang, J. B., Lin, J. X., ... & Li, P. (2020). circ‐CEP85L suppresses the proliferation and invasion of gastric cancer by regulating NFKBIA expression via miR‐942‐5p. Journal of Cellular Physiology, 235(9), 6287-6299.

Sogkas, G., Adriawan, I. R., Ringshausen, F. C., Baumann, U., Schroeder, C., Klemann, C., ... & Atschekzei, F. (2020). A novel NFKBIA variant substituting serine 36 of IκBα causes immunodeficiency with warts, bronchiectasis and juvenile rheumatoid arthritis in the absence of ectodermal dysplasia. Clinical Immunology, 210, 108269.

Coto, E., Reguero, J. R., Avanzas, P., Pascual, I., Martín, M., Hevia, S., ... & Gómez, J. (2019). Gene variants in the NF-KB pathway (NFKB1, NFKBIA, NFKBIZ) and risk for early-onset coronary artery disease. Immunology letters, 208, 39-43.

Li, L., & Zhang, Z. T. (2019). Genetic association between NFKBIA and NFKB1 gene polymorphisms and the susceptibility to head and neck cancer: a meta-analysis. Disease markers, 2019.

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For research use only. Not intended for any clinical use.

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