Mouse Anti-NFKBIE Recombinant Antibody (3F12) (CBMAB-N2312-WJ)

This product is a Mouse antibody that recognizes NFKBIE. The antibody 3F12 can be used for immunoassay techniques such as: ELISA, WB.
See all NFKBIE antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

3F12

Application

ELISA, WB

Basic Information

Specificity

Human

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, epsilon

Introduction

The protein encoded by this gene binds to components of NF-kappa-B, trapping the complex in the cytoplasm and preventing it from activating genes in the nucleus. Phosphorylation of the encoded protein targets it for destruction by the ubiquitin pathway, which activates NF-kappa-B by making it available to translocate to the nucleus. [provided by RefSeq, Sep 2011]

Entrez Gene ID

4794

UniProt ID

O00221

Alternative Names

NFKB Inhibitor Epsilon; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells Inhibitor, Epsilon; NF-Kappa-B Inhibitor Epsilon; I-Kappa-B-Epsilon; IkappaBepsilon; NF-Kappa-BIE; IkB-Epsilon; IkB-E; IKBE;

Function

Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.

Biological Process

Cytoplasmic sequestering of transcription factorManual Assertion Based On ExperimentTAS:ProtInc

Cellular Location

Cytoplasm

PTM

Serine phosphorylated; followed by proteasome-dependent degradation.

References

Qian, L., Li, H., Tu, J., Zhang, Y., Dai, T., Sun, S., ... & Zheng, L. (2022). NFKBIE Is a Predictive Factor of Survival and Is Correlated With Immune Infiltration, Antigen Processing, And Presentation In Hepatocellular Carcinoma.

Bonato, A., Bomben, R., Chakraborty, S., Felician, G., Martines, C., Zucchetto, A., ... & Efremov, D. G. (2021). Chronic Lymphocytic Leukemia Cells with Mutated Nfkbie Are Positively Selected By Microenvironmental Signals and Display Reduced Sensitivity to Ibrutinib Treatment. Blood, 138, 248.

Della-Valle, V., Roos-Weil, D., Scourzic, L., Mouly, E., Aid, Z., Darwiche, W., ... & Ghamlouch, H. (2020). Nfkbie-deficiency leads to increased susceptibility to develop B-cell lymphoproliferative disorders in aged mice. Blood Cancer Journal, 10(3), 38.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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