Mouse Anti-NFYA Recombinant Antibody (3F9) (CBMAB-N2326-WJ)

This product is a Mouse antibody that recognizes NFYA. The antibody 3F9 can be used for immunoassay techniques such as: ELISA, WB.
See all NFYA antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Rat

Clone

3F9

Antibody Isotype

IgM, κ

Application

ELISA, WB

Basic Information

Specificity

Human, Rat

Antibody Isotype

IgM, κ

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Nuclear Transcription Factor Y Subunit Alpha

Introduction

The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. [provided by RefSeq, Jul 2008]

Entrez Gene ID

4800

UniProt ID

P23511

Alternative Names

Nuclear Transcription Factor Y Subunit Alpha; Nuclear Transcription Factor Y Subunit A; Nuclear Transcription Factor Y, Alpha; NF-YA; CCAAT-Binding Transcription Factor Subunit B; CAAT-Box DNA Binding Protein Subunit A; CAAT Box DNA-Binding Protein Subunit A;

Function

Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors. NF-YA positively regulates the transcription of the core clock component ARNTL/BMAL1.

Biological Process

Histone H3-K4 trimethylationManual Assertion Based On ExperimentIDA:ComplexPortal
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:ComplexPortal
Positive regulation of transcription, DNA-templatedISS:UniProtKB
Regulation of histone acetylationManual Assertion Based On ExperimentIDA:ComplexPortal
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:UniProtKB
Rhythmic processIEA:UniProtKB-KW
Transcription by RNA polymerase IIManual Assertion Based On ExperimentTAS:ProtInc

Cellular Location

Nucleus

References

ERGÜN, S., Ferda, A. R. I., Benli, E., Altay, D. U., Noyan, T., Erdem, H., & ARICI, Y. K. (2022). YY1 and NFYA: Potential tr-KIT Specific Transcription Factors in Prostate Cancer. Middle Black Sea Journal of Health Science, 8(2), 202-207.

Liu, R., Wu, M., Liu, H. L., Gao, Y. M., Chen, J., Yan, H. W., & Xiang, Y. (2021). Genome‐wide identification and expression analysis of the NF‐Y transcription factor family in Populus. Physiologia plantarum, 171(3), 309-327.

Li, Y., Xiao, X., Chen, H., Chen, Z., Hu, K., & Yin, D. (2020). Transcription factor NFYA promotes G1/S cell cycle transition and cell proliferation by transactivating cyclin D1 and CDK4 in clear cell renal cell carcinoma. American Journal of Cancer Research, 10(8), 2446.

Manna, D., & Singh, U. (2019). Nuclear Factor Y (NF-Y) modulates Encystation in Entamoeba via stage-specific expression of the NF-YB and NF-YC Subunits. MBio, 10(3), 10-1128.

Wang, P., Zheng, Y., Guo, Y., Chen, X., Sun, Y., Yang, J., & Ye, N. (2019). Identification, expression, and putative target gene analysis of nuclear factor-Y (NF-Y) transcription factors in tea plant (Camellia sinensis). Planta, 250, 1671-1686.

Bie, L. Y., Li, D., Mu, Y., Wang, S., Chen, B. B., Lyu, H. F., ... & Luo, S. X. (2019). Analysis of cyclin E co-expression genes reveals nuclear transcription factor Y subunit alpha is an oncogene in gastric cancer. Chronic Diseases and Translational Medicine, 5(01), 44-52.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-NFYA Recombinant Antibody (G-2)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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