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Mouse Anti-NME4 Recombinant Antibody (1A5) (CBMAB-N2784-WJ)

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Basic Information

Host Animal
Mouse
Clone
1A5
Application
FC, IF, IHC-P, WB
Specificity
Human
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
non-metastatic cells 4, protein expressed in
Introduction
The nucleoside diphosphate (NDP) kinases (EC 2.7.4.6) are ubiquitous enzymes that catalyze transfer of gamma-phosphates, via a phosphohistidine intermediate, between nucleoside and dioxynucleoside tri- and diphosphates. The enzymes are products of the nm23 gene family, which includes NME4 (Milon et al., 1997 [PubMed 9099850]).[supplied by OMIM, May 2008]
Entrez Gene ID
4833
UniProt ID
O00746
Alternative Names
NME/NM23 Nucleoside Diphosphate Kinase 4; Non-Metastatic Cells 4, Protein Expressed In; Nucleoside Diphosphate Kinase D; NDP Kinase, Mitochondrial; EC 2.7.4.6; Nm23-H4; NDPKD;
Function
Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Through the catalyzed exchange of gamma-phosphate between di- and triphosphonucleosides participates in regulation of intracellular nucleotide homeostasis (PubMed:10799505).
Binds to anionic phospholipids, predominantly to cardiolipin; the binding inhibits its phosphotransfer activity (PubMed:18635542, PubMed:23150663).
Acts as mitochondria-specific NDK; its association with cardiolipin-containing mitochondrial inner membrane is coupled to respiration suggesting that ADP locally regenerated in the mitochondrion innermembrane space by its activity is directly taken up via ANT ADP/ATP translocase into the matrix space to stimulate respiratory ATP regeneration (PubMed:18635542).
Proposed to increase GTP-loading on dynamin-related GTPase OPA1 in mitochondria (PubMed:24970086).
In vitro can induce liposome cross-linking suggesting that it can cross-link inner and outer membranes to form contact sites, and promotes intermembrane migration of anionic phosphoplipids. Promotes the redistribution of cardiolipin between the mitochondrial inner membrane and outer membrane which is implicated in pro-apoptotic signaling (PubMed:18635542, PubMed:17028143, PubMed:23150663).
Biological Process
CTP biosynthetic processIEA:InterPro
GTP biosynthetic processIEA:InterPro
Lipid transportManual Assertion Based On ExperimentIDA:BHF-UCL
Nucleoside metabolic processManual Assertion Based On ExperimentTAS:ProtInc
UTP biosynthetic processIEA:InterPro
Cellular Location
Mitochondrion intermembrane space
Mitochondrion matrix
Predominantly localized in the mitochondrion intermembrane space (PubMed:18635542).
Colocalizes with OPA1 in mitochondria (PubMed:24970086).

Yu, J. H., Jung, Y. J., Kim, M. S., Cho, S. R., & Kim, Y. H. (2023). Differential Expression of NME4 in Trophoblast Stem-Like Cells and Peripheral Blood Mononuclear Cells of Normal Pregnancy and Preeclampsia. Journal of Korean Medical Science, 38(16).

Zhengyu, F. A. N. G., Qiongxiang, G. E., Zhi, C. H. E. N., Haixin, Q. I., & ZHENG, B. (2023). NME4 promotes cell malignant process by targeting SMAD2 in colorectal cancer.

Adam, K., Ning, J., Reina, J., & Hunter, T. (2020). NME/NM23/NDPK and histidine phosphorylation. International journal of molecular sciences, 21(16), 5848.

Zheng, S., Liu, Q., Liu, T., Yang, L., Zhang, Q., Shen, T., ... & Lu, X. (2020). NME4 modulates PD-L1 expression via the STAT3 signaling pathway in squamous cell carcinoma. Biochemical and Biophysical Research Communications, 526(1), 29-34.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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