Mouse Anti-NMNAT2 Recombinant Antibody (2E4) (CBMAB-N2815-WJ)

Name: Mouse Anti-NMNAT2 Recombinant Antibody (2E4)
SKU: CBMAB-N2815-WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

2E4

Application

ELISA, WB

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.2

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

nicotinamide nucleotide adenylyltransferase 2

Introduction

This gene product belongs to the nicotinamide mononucleotide adenylyltransferase (NMNAT) enzyme family, members of which catalyze an essential step in NAD (NADP) biosynthetic pathway. Unlike the other human family member, which is localized to the nucleus, and is ubiquitously expressed; this enzyme is cytoplasmic, and is predominantly expressed in the brain. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Entrez Gene ID

23057

UniProt ID

Q9BZQ4

Alternative Names

Nicotinamide Nucleotide Adenylyltransferase 2; Nicotinamide Mononucleotide Adenylyltransferase 2; Nicotinate-Nucleotide Adenylyltransferase 2; NMN/NaMN Adenylyltransferase 2; NaMN Adenylyltransferase 2; NMN Adenylyltransferase 2; C1orf15; Nicotinamide/Nicotinic Acid Mononucleotide Adenylyltransferase 2;

Function

Nicotinamide/nicotinate-nucleotide adenylyltransferase that acts as an axon maintenance factor (By similarity).
Catalyzes the formation of NAD+ from nicotinamide mononucleotide (NMN) and ATP (PubMed:16118205, PubMed:17402747).
Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate but with a lower efficiency (PubMed:16118205, PubMed:17402747).
Cannot use triazofurin monophosphate (TrMP) as substrate (PubMed:16118205, PubMed:17402747).
Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD+ (PubMed:16118205, PubMed:17402747).
For the pyrophosphorolytic activity prefers NAD+, NADH and NaAD as substrates and degrades nicotinic acid adenine dinucleotide phosphate (NHD) less effectively (PubMed:16118205, PubMed:17402747).
Fails to cleave phosphorylated dinucleotides NADP+, NADPH and NaADP+ (PubMed:16118205, PubMed:17402747).
Axon survival factor required for the maintenance of healthy axons: acts by delaying Wallerian axon degeneration, an evolutionarily conserved process that drives the loss of damaged axons (By similarity).

Biological Process

NAD biosynthetic processManual Assertion Based On ExperimentIBA:GO_Central
Nucleotide biosynthetic process1 PublicationIC:UniProtKB

Cellular Location

Golgi apparatus membrane
Cytoplasmic vesicle membrane
Cytoplasm
Cell projection, axon
Delivered to axons with Golgi-derived cytoplasmic vesicles.

PTM

Degraded in response to injured neurite. Degradation is probably caused by ubiquitination by MYCBP2 (By similarity).
Ubiquitinated on threonine and/or serine residues by MYCBP2; consequences of threonine and/or serine ubiquitination are however unclear (PubMed:29643511).
Palmitoylated; palmitoylation is required for membrane association.

Yang, S., Niou, Z. X., Enriquez, A., LaMar, J., Huang, J. Y., Ling, K., ... & Lu, H. C. (2024). NMNAT2 supports vesicular glycolysis via NAD homeostasis to fuel fast axonal transport. Molecular Neurodegeneration, 19(1), 13.

Li, W., Gao, M., Hu, C., Chen, X., & Zhou, Y. (2023). NMNAT2: An important metabolic enzyme affecting the disease progression. Biomedicine & Pharmacotherapy, 158, 114143.

Chen, H., Zhang, X., Liao, N., Ji, Y., Mi, L., Gan, Y., ... & Wen, F. (2023). Downregulation of SIRT6 and NMNAT2 is associated with proliferative diabetic retinopathy. Molecular Vision, 29, 160.

Fang, F., Zhuang, P., Feng, X., Liu, P., Liu, D., Huang, H., ... & Hu, Y. (2022). NMNAT2 is downregulated in glaucomatous RGCs, and RGC-specific gene therapy rescues neurodegeneration and visual function. Molecular Therapy, 30(4), 1421-1431.

Cheng, X. S., Shi, F. X., Zhao, K. P., Lin, W., Li, X. Y., Zhang, J., ... & Zhou, X. W. (2021). Nmnat2 attenuates amyloidogenesis and up-regulates ADAM10 in AMPK activity-dependent manner. Aging (Albany NY), 13(20), 23620.

Loreto, A., Hill, C. S., Hewitt, V. L., Orsomando, G., Angeletti, C., Gilley, J., ... & Coleman, M. P. (2020). Mitochondrial impairment activates the Wallerian pathway through depletion of NMNAT2 leading to SARM1-dependent axon degeneration. Neurobiology of disease, 134, 104678.

Lukacs, M., Gilley, J., Zhu, Y., Orsomando, G., Angeletti, C., Liu, J., ... & Stottmann, R. W. (2019). Severe biallelic loss-of-function mutations in nicotinamide mononucleotide adenylyltransferase 2 (NMNAT2) in two fetuses with fetal akinesia deformation sequence. Experimental neurology, 320, 112961.

Huppke, P., Wegener, E., Gilley, J., Angeletti, C., Kurth, I., Drenth, J. P., ... & Coleman, M. P. (2019). Homozygous NMNAT2 mutation in sisters with polyneuropathy and erythromelalgia. Experimental Neurology, 320, 112958.

Gilley, J., Mayer, P. R., Yu, G., & Coleman, M. P. (2019). Low levels of NMNAT2 compromise axon development and survival. Human molecular genetics, 28(3), 448-458.

Wu, X., Hu, F., Zeng, J., Han, L., Qiu, D., Wang, H., ... & Wang, Q. (2019). NMNAT2‐mediated NAD+ generation is essential for quality control of aged oocytes. Aging cell, 18(3), e12955.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
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For research use only. Not intended for any clinical use.

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