Mouse Anti-NMNAT3 Recombinant Antibody (B-9) (CBMAB-N2819-WJ)

This product is a Mouse antibody that recognizes NMNAT3. The antibody B-9 can be used for immunoassay techniques such as: WB, IP, IF, ELISA.
See all NMNAT3 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

mouse

Clone

B-9

Application

WB, IP, IF, ELISA

Basic Information

Specificity

mouse

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Nicotinamide Nucleotide Adenylyltransferase 3

Introduction

This gene encodes a member of the nicotinamide/nicotinic acid mononucleotide adenylyltransferase family. These enzymes use ATP to catalyze the synthesis of nicotinamide adenine dinucleotide or nicotinic acid adenine dinucleotide from nicotinamide mononucleotide or nicotinic acid mononucleotide, respectively. The encoded protein is localized to mitochondria and may also play a neuroprotective role as a molecular chaperone. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]

Entrez Gene ID

349565

UniProt ID

Q96T66

Alternative Names

Nicotinamide Nucleotide Adenylyltransferase 3; Nicotinate-Nucleotide Adenylyltransferase 3; Pyridine Nucleotide Adenylyltransferase 3; NMN/NaMN Adenylyltransferase 3; NaMN Adenylyltransferase 3; NMN Adenylyltransferase 3; PNAT-3; Nicotinamide/Nicotinic Acid Mononucleotide Adenylyltransferase 3;

Function

Catalyzes the formation of NAD+ from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency. Can use triazofurin monophosphate (TrMP) as substrate. Can also use GTP and ITP as nucleotide donors. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD+. For the pyrophosphorolytic activity, can use NAD+, NADH, NaAD, nicotinic acid adenine dinucleotide phosphate (NHD), nicotinamide guanine dinucleotide (NGD) as substrates. Fails to cleave phosphorylated dinucleotides NADP+, NADPH and NaADP+. Protects against axonal degeneration following injury.

Biological Process

NAD biosynthetic processManual Assertion Based On ExperimentIBA:GO_Central
Nucleotide biosynthetic process1 PublicationIC:UniProtKB

Cellular Location

Mitochondrion

References

Wang, T., Zhang, F., Peng, W., Wang, L., Zhang, J., Dong, W., ... & Gong, Y. (2022). Overexpression of NMNAT3 improves mitochondrial function and enhances antioxidative stress capacity of bone marrow mesenchymal stem cells via the NAD+-Sirt3 pathway. Bioscience Reports, 42(1), BSR20211005.

Parsons, R. B., Kocinaj, A., Ruiz Pulido, G., Prendergast, S. A., Parsons, A. E., Facey, P. D., & Hirth, F. (2022). Alpha-synucleinopathy reduces NMNAT3 protein levels and neurite formation that can be rescued by targeting the NAD+ pathway. Human Molecular Genetics, 31(17), 2918-2933.

Mahmood, A., Yaku, K., Hikosaka, K., Gulshan, M., Inoue, S. I., Kobayashi, F., & Nakagawa, T. (2022). Nmnat3 deficiency in hemolytic anemia exacerbates malaria infection. Biochemical and Biophysical Research Communications, 637, 58-65.

Wang, T., Peng, W., Zhang, F., Wang, L., Zhang, J., Dong, W., ... & Gong, Y. (2020). NMNAT3 improves mitochondrial function and enhances BMSCs anti-oxidative stress through the NAD+-Sirt3 pathway.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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