Mouse Anti-NOLC1 Recombinant Antibody (KAB40) (CBMAB-N2908-WJ)

Name: Mouse Anti-NOLC1 Recombinant Antibody (KAB40)
SKU: CBMAB-N2908-WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

KAB40

Application

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

nucleolar and coiled-body phosphoprotein 1

Introduction

Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification (PubMed:10567578, PubMed:26399832). Required for neural crest specification: following monoubiquitination by the BCR(KBTBD8) complex, associates with TCOF1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification (PubMed:26399832). Involved in nucleologenesis, possibly by playing a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus (PubMed:9016786). It has intrinsic GTPase and ATPase activities (PubMed:9016786).

Entrez Gene ID

Human	9221
Mouse	70769
Rat	64896

UniProt ID

Human	Q14978
Mouse	E9Q5C9
Rat	P41777

Alternative Names

Nucleolar And Coiled-Body Phosphoprotein 1; Hepatitis C Virus NS5A-Transactivated Protein 13; HCV NS5A-Transactivated Protein 13; 140 KDa Nucleolar Phosphoprotein; Nucleolar Phosphoprotein P130; Nucleolar 130 KDa Protein; NS5ATP13; NOPP140;

Function

Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification (PubMed:10567578, PubMed:26399832).
Required for neural crest specification: following monoubiquitination by the BCR(KBTBD8) complex, associates with TCOF1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification (PubMed:26399832).
Involved in nucleologenesis, possibly by playing a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus (PubMed:9016786).
It has intrinsic GTPase and ATPase activities (PubMed:9016786).

Biological Process

Box H/ACA RNA metabolic processIEA:Ensembl
Mitotic cell cycleManual Assertion Based On ExperimentTAS:ProtInc
Neural crest cell developmentManual Assertion Based On ExperimentIMP:UniProtKB
Neural crest formationManual Assertion Based On ExperimentIMP:UniProtKB
Nucleolus organizationIEA:Ensembl
Positive regulation of cell population proliferationIEA:Ensembl
Positive regulation of transcription, DNA-templatedIEA:Ensembl
Regulation of protein import into nucleusIEA:Ensembl
Regulation of translationManual Assertion Based On ExperimentIMP:UniProtKB
Response to osmotic stressIEA:Ensembl
rRNA processingManual Assertion Based On ExperimentTAS:ProtInc

PTM

Undergoes rapid and massive phosphorylation/dephosphorylation cycles on CK2 and PKC sites. NOLC1 is one of the mostly phosphorylated proteins in the cell.
Ubiquitinated. Monoubiquitination by the BCR(KBTBD8) complex promotes the formation of a NOLC1-TCOF1 complex that acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification (PubMed:26399832).
Pyrophosphorylated by 5-diphosphoinositol pentakisphosphate (5-IP7). Serine pyrophosphorylation is achieved by Mg2+-dependent, but enzyme independent transfer of a beta-phosphate from a inositol pyrophosphate to a pre-phosphorylated serine residue.

Choi, H., Kim, H. D., Choi, Y. W., Lim, H., Kim, K. W., Kim, K. S., ... & Kim, C. H. (2023). T7 phage display reveals NOLC1 as a GM3 binding partner in human breast cancer MCF-7 cells. Archives of Biochemistry and Biophysics, 750, 109810.

Zhai, F., Wang, J., Luo, X., Ye, M., & Jin, X. (2023). Roles of NOLC1 in cancers and viral infection. Journal of Cancer Research and Clinical Oncology, 1-16.

Sun, Z., Zhang, Q., Lv, J., Sun, Y., Feng, Z., Zhang, M., ... & Ren, S. Y. (2023). High expression of NOLC1 as an independent prognostic factor for survival in patients with colorectal cancer. Journal of Cancer Research and Clinical Oncology, 149(17), 15697-15712.

Sochacka, M., Karelus, R., Opalinski, L., Krowarsch, D., Biadun, M., Otlewski, J., & Zakrzewska, M. (2022). FGF12 is a novel component of the nucleolar NOLC1/TCOF1 ribosome biogenesis complex. Cell Communication and Signaling, 20(1), 182.

Kim, W., Yeo, D. Y., Choi, S. K., Kim, H. Y., Lee, S. W., Ashim, J., ... & Park, S. (2022). NOLC1 knockdown suppresses prostate cancer progressions by reducing AKT phosphorylation and β-catenin accumulation. Biochemical and Biophysical Research Communications, 635, 99-107.

Luo, J. H., Liu, S., Tao, J., Ren, B. G., Luo, K., Chen, Z. H., ... & Yu, Y. P. (2021). Pten-NOLC1 fusion promotes cancers involving MET and EGFR signalings. Oncogene, 40(6), 1064-1076.

Chen, S., Wu, W., Li, Q. H., Xie, B. M., Shen, F., Du, Y. P., ... & Zhao, Y. (2021). Circ-NOLC1 promotes epithelial ovarian cancer tumorigenesis and progression by binding ESRP1 and modulating CDK1 and RhoA expression. Cell death discovery, 7(1), 22.

Wu, C. Y., Li, L., Chen, S. L., Yang, X., Zhang, C. Z., & Cao, Y. (2021). A Zic2/Runx2/NOLC1 signaling axis mediates tumor growth and metastasis in clear cell renal cell carcinoma. Cell Death & Disease, 12(4), 319.

Kong, F., Shang, Y., Diao, X., Huang, J., & Liu, H. (2021). Knockdown of NOLC1 inhibits PI3K-AKT pathway to improve the poor prognosis of esophageal carcinoma. Journal of oncology, 2021, 1-10.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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