Mouse Anti-NOP2 Recombinant Antibody (E-7) (CBMAB-N2933-WJ)

Name: Mouse Anti-NOP2 Recombinant Antibody (E-7)
SKU: CBMAB-N2933-WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

E-7

Application

WB, IP, IF, ELISA

Specificity

Human

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

NOP2 Nucleolar Protein

Introduction

Involved in ribosomal large subunit assembly (PubMed:24120868). S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C(5) position of cytosine 4447 in 28S rRNA (Probable). May play a role in the regulation of the cell cycle and the increased nucleolar activity that is associated with the cell proliferation (Probable).

Entrez Gene ID

4839

UniProt ID

P46087

Alternative Names

NOP2 Nucleolar Protein; Proliferation-Associated Nucleolar Protein P120; Proliferating-Cell Nucleolar Antigen P120; Nucleolar Protein 1, 120kDa; Nucleolar Protein 2 Homolog; NSUN1; NOL1; Probable 28S RRNA (Cytosine(4447)-C(5))-Methyltransferase; Putative Ribosomal RNA Methyltransferase NOP2; NOP2 Nucleolar Protein Homolog (Yeast); NOL1/NOP2/Sun Domain Family, Member 1;

Function

Involved in ribosomal large subunit assembly (PubMed:24120868).
S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C5 position of cytosine 4447 in 28S rRNA (Probable). May play a role in the regulation of the cell cycle and the increased nucleolar activity that is associated with the cell proliferation (Probable).

Biological Process

Maturation of LSU-rRNAManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of cell population proliferationManual Assertion Based On ExperimentIDA:GO_Central
Regulation of signal transduction by p53 class mediatorManual Assertion Based On ExperimentIMP:UniProtKB
Ribosomal large subunit assemblyManual Assertion Based On ExperimentIMP:UniProtKB
RNA methylationManual Assertion Based On ExperimentIBA:GO_Central
rRNA base methylationManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Nucleus, nucleolus

PTM

Citrullinated by PADI4.

Wang, M., Cheng, R., He, H., Han, Z., Zhang, Y., & Wu, Q. (2023). N4-acetylcytidine of Nop2 mRNA is required for the transition of morula-to-blastocyst. Cellular and Molecular Life Sciences, 80(10), 307.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-NOP2 Recombinant Antibody (CBWJN-0170) (CAT#: CBMAB-N2934-WJ)

Isotype control

For research use only. Not intended for any clinical use.

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