• Home
  • NOTUM
  • Mouse Anti-NOTUM Recombinant Antibody (CBWJN-1484)

Mouse Anti-NOTUM Recombinant Antibody (CBWJN-1484) (CBMAB-N3061-WJ)

See all NOTUM antibodies
Go to compare Compare Online Inquiry
Datasheet
SDS
Request for COA
Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal
Mouse
Clone
CBWJN-1484
Application
WB
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
Notum, Palmitoleoyl-Protein Carboxylesterase
Introduction
Carboxylesterase that acts as a key negative regulator of the Wnt signaling pathway by specifically mediating depalmitoleoylation of WNT proteins. Serine palmitoleoylation of WNT proteins is required for efficient binding to frizzled receptors (PubMed:25731175).
Entrez Gene ID
147111
UniProt ID
Q6P988
Alternative Names
Notum, Palmitoleoyl-Protein Carboxylesterase; [Wnt Protein] O-Palmitoleoyl-L-Serine Hydrolase; HNOTUM; Notum Pectinacetylesterase Homolog (Drosophila); Palmitoleoyl-Protein Carboxylesterase NOTUM; Notum Pectinacetylesterase Homolog; Protein Notum Homolog; EC 3.1.1.98;
Function
Carboxylesterase that acts as a key negative regulator of the Wnt signaling pathway by specifically mediating depalmitoleoylation of WNT proteins. Serine palmitoleoylation of WNT proteins is required for efficient binding to frizzled receptors (PubMed:25731175).
Biological Process
Bone developmentManual Assertion Based On ExperimentIMP:MGI
Negative regulation of canonical Wnt signaling pathwayManual Assertion Based On ExperimentIBA:GO_Central
Negative regulation of Wnt signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Protein depalmitoleylationManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of bone mineralizationManual Assertion Based On ExperimentIDA:MGI
Wnt signaling pathwayIEA:UniProtKB-KW
Cellular Location
Secreted

Song, L., Sun, M., Shi, J., Tian, Z., Song, Y., Liu, H., ... & Ge, G. (2023). Rational Construction of a Novel Bioluminescent Substrate for Sensing the Tumor-Associated Hydrolase Notum. Analytical Chemistry, 95(13), 5489-5493.

Yuan, Y. F., Wang, S., Zhou, H., Tang, B. B., Liu, Y., Huang, H., ... & Shi, X. L. (2022). Exploratory study of sea buckthorn enhancing QiangGuYin efficacy by inhibiting CKIP-1 and Notum activating the Wnt/β-catenin signaling pathway and analysis of active ingredients by molecular docking. Frontiers in Pharmacology, 13, 994995.

Bayle, E. D., Svensson, F., Atkinson, B. N., Steadman, D., Willis, N. J., Woodward, H. L., ... & Fish, P. V. (2021). Carboxylesterase notum is a druggable target to modulate Wnt signaling. Journal of medicinal chemistry, 64(8), 4289-4311.

Zhao, Y., Schuhmacher, L. N., Roberts, M., Kakugawa, S., Bineva-Todd, G., Howell, S., ... & Jones, E. Y. (2021). Notum deacylates octanoylated ghrelin. Molecular Metabolism, 49, 101201.

Flanagan, D. J., Pentinmikko, N., Luopajärvi, K., Willis, N. J., Gilroy, K., Raven, A. P., ... & Sansom, O. J. (2021). NOTUM from Apc-mutant cells biases clonal competition to initiate cancer. Nature, 594(7863), 430-435.

Zhao, Y., Jolly, S., Benvegnu, S., Jones, E. Y., & Fish, P. V. (2021). Small-molecule inhibitors of carboxylesterase Notum. Future Medicinal Chemistry, 13(11), 1001-1015.

Mahy, W., Patel, M., Steadman, D., Woodward, H. L., Atkinson, B. N., Svensson, F., ... & Fish, P. V. (2020). Screening of a custom-designed acid fragment library identifies 1-phenylpyrroles and 1-phenylpyrrolidines as inhibitors of Notum carboxylesterase activity. Journal of medicinal chemistry, 63(17), 9464-9483.

Mizrak, D., Bayin, N. S., Yuan, J., Liu, Z., Suciu, R. M., Niphakis, M. J., ... & Sims, P. A. (2020). Single-cell profiling and SCOPE-Seq reveal lineage dynamics of adult ventricular-subventricular zone neurogenesis and NOTUM as a key regulator. Cell reports, 31(12).

Pentinmikko, N., Iqbal, S., Mana, M., Andersson, S., Cognetta III, A. B., Suciu, R. M., ... & Katajisto, P. (2019). Notum produced by Paneth cells attenuates regeneration of aged intestinal epithelium. Nature, 571(7765), 398-402.

Brommage, R., Liu, J., Vogel, P., Mseeh, F., Thompson, A. Y., Potter, D. G., ... & Liu, Q. (2019). NOTUM inhibition increases endocortical bone formation and bone strength. Bone Research, 7(1), 2.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-NOTUM Recombinant Antibody (CBWJN-1484)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry
Request bulk or custom quote Second antibody and isotype control

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

online inquiry
Online Inquiry