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Rabbit Anti-NPRL2 Recombinant Antibody (D8K3X) (CBMAB-CP2903-LY)

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Datasheet
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Basic Information

Host Animal
Rabbit
Clone
D8K3X
Application
WB, IP
Immunogen
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala211 of human NPRL2 protein.
Specificity
Human, Mouse, Rat, Monkey
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
NPR2 Like, GATOR1 Complex Subunit
Introduction
As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. The GATOR1 complex is negatively regulated by GATOR2 the other GATOR subcomplex in this amino acid-sensing branch of the TORC1 pathway.
Entrez Gene ID
Human10641
Mouse56032
Rat363138
Monkey701924
UniProt ID
HumanQ8WTW4
MouseQ9WUE4
RatD3ZPN1
MonkeyA0A5F7ZGF3
Alternative Names
NPR2 Like; GATOR1 Complex Subunit; Tumor Suppressor Candidate 4; Nitrogen Permease Regulator 2-Like Protein; NPR2-Like Protein; Gene 21 Protein; G21 Protein; TUSC4; Homologous To Yeast Nitrogen Permease (Candidate Tumor Suppressor);
Function
As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. The GATOR1 complex is negatively regulated by GATOR2 the other GATOR subcomplex in this amino acid-sensing branch of the TORC1 pathway.
Suppresses Src-dependent tyrosine phosphorylation and activation of PDPK1 and its downstream signaling. Down-regulates PDPK1 kinase activity by interfering with tyrosine phosphorylation at 'Tyr-9', 'Tyr-373' and 'Tyr-376' residues. May act as a tumor suppressor. Suppresses cell growth and enhances sensitivity to various anticancer drugs.
Biological Process
Cellular response to amino acid starvationManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of kinase activityManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of TOR signalingManual Assertion Based On ExperimentIMP:SGD
Negative regulation of TORC1 signalingManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of autophagyManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Lysosome membrane
Localization to lysosomes is amino acid-independent.
Involvement in disease
Epilepsy, familial focal, with variable foci 2 (FFEVF2):
An autosomal dominant form of epilepsy characterized by focal seizures arising from different cortical regions, including the temporal, frontal, parietal, and occipital lobes. Seizure types commonly include temporal lobe epilepsy, frontal lobe epilepsy, and nocturnal frontal lobe epilepsy. Some patients may have intellectual disability or autism spectrum disorders. Seizure onset usually occurs in the first or second decades, although later onset has been reported, and there is phenotypic variability within families. A subset of patients have structural brain abnormalities. Penetrance of the disorder is incomplete.

Zhang, H., Deng, J., Gao, Z., Wang, Y., Zhao, F., Zhao, H., & Fang, F. (2023). Clinical phenotype and genotype of NPRL2-related epilepsy: Four cases reports and literature review. Seizure: European Journal of Epilepsy.

Jiang, C., Liu, J., He, S., Xu, W., Huang, R., Pan, W., ... & Wei, W. (2023). PRMT1 orchestrates with SAMTOR to govern mTORC1 methionine sensing via Arg-methylation of NPRL2. Cell Metabolism, 35(12), 2183-2199.

Ishida, S., Zhao, D., Sawada, Y., Hiraoka, Y., Mashimo, T., & Tanaka, K. (2022). Dorsal telencephalon-specific Nprl2-and Nprl3-knockout mice: novel mouse models for GATORopathy. Human Molecular Genetics, 31(9), 1519-1530.

Zhang, J., Shen, Y., Yang, Z., Yang, F., Li, Y., Yu, B., ... & Gan, J. (2022). A splicing variation in NPRL2 causing familial focal epilepsy with variable foci: additional cases and literature review. Journal of Human Genetics, 67(2), 79-85.

Wang, Y. C., Tsai, M. C., Chen, Y. S., Hsieh, P. M., Hung, C. M., Lin, H. Y., ... & Lin, C. W. (2022). NPRL2 down‐regulation facilitates the growth of hepatocellular carcinoma via the mTOR pathway and autophagy suppression. Hepatology Communications, 6(12), 3563-3577.

Sun, Y., Wan, L., Yan, H., Li, Z., & Yang, G. (2021). Phenotypic and genotypic characterization of NPRL2-related epilepsy: two case reports and literature review. Frontiers in Neurology, 12, 780799.

Tang, Y., Jiang, L., Zhao, X., Hu, D., Zhao, G., Luo, S., ... & Tang, W. (2021). FOXO1 inhibits prostate cancer cell proliferation via suppressing E2F1 activated NPRL2 expression. Cell Biology International, 45(12), 2510-2520.

Luo, S., Shao, L., Chen, Z., Hu, D., Jiang, L., & Tang, W. (2020). NPRL2 promotes docetaxel chemoresistance in castration resistant prostate cancer cells by regulating autophagy through the mTOR pathway. Experimental Cell Research, 390(2), 111981.

Xi, J., Cai, J., Cheng, Y., Fu, Y., Wei, W., Zhang, Z., ... & Wei, Y. (2019). The TORC1 inhibitor Nprl2 protects age-related digestive function in Drosophila. Aging (Albany NY), 11(21), 9811.

Chen, Z., Jiang, Q., Zhu, P., Chen, Y., Xie, X., Du, Z., ... & Tang, W. (2019). NPRL2 enhances autophagy and the resistance to Everolimus in castration‐resistant prostate cancer. The Prostate, 79(1), 44-53.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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